Acetaldehyde binds to liver cell membranes without affecting membrane function.

Acetaldehyde is a major metabolic product of ethanol and is found in high concentrations in the serum during alcohol abuse. The effects of acetaldehyde on isolated rat liver cells and on purified hepatocyte plasma membrane vesicles have been studied. In concentrations of 0-10 millimolar acetaldehyde has been shown to have no detectable effect on either hepatocyte metabolism or gross membrane function and is therefore unlikely to act as a direct metabolic poison. Acetaldehyde, however, is shown to bind to hepatocyte membranes via intermediary Schiff's base formation. The adduction of acetaldehyde to liver cell plasma membranes may have an effect on membrane structure. These findings are consistent with the hypothesis that any injurious effect of acetaldehyde on the liver may be mediated via the immune system rather than being a direct effect on cell metabolism.

[1]  K. Jungermann,et al.  Regulation of Hepatic Metabolism , 1986, Springer US.

[2]  H. J. Sips,et al.  Orientation of rat-liver plasma membrane vesicles. A biochemical and ultrastructural study. , 1982, Biochimica et biophysica acta.

[3]  M. Samson,et al.  Plasma membrane vesicles from isolated hepatocytes retain the increase of amino acid transport induced by dibutyryl cyclic AMP in intact cells. , 1982, Biochimica et biophysica acta.

[4]  C. M. Leevy,et al.  Ethanol, immune reactions and the digestive system. , 1981, Clinics in gastroenterology.

[5]  W. Fantl,et al.  Acetaldehyde adducts with hemoglobin. , 1981, The Journal of clinical investigation.

[6]  D. Dearborn,et al.  Protein labeling by reductive methylation with sodium cyanoborohydride: effect of cyanide and metal ions on the reaction. , 1980, Analytical biochemistry.

[7]  C. Eriksson.  Elevated blood acetaldehyde levels in alcoholics and their relatives: a reevaluation , 1980, Science.

[8]  J. Sear,et al.  Cytotoxicity of i.v. anaesthetic agents on the isolated rat hepatocyte. , 1979, British journal of anaesthesia.

[9]  D. Dearborn,et al.  Labeling of proteins by reductive methylation using sodium cyanoborohydride. , 1979, The Journal of biological chemistry.

[10]  S. Joseph,et al.  Characteristics of the transport of alanine, serine and glutamine across the plasma membrane of isolated rat liver cells. , 1978, The Biochemical journal.

[11]  H. J. Sips,et al.  Sodium-dependent alanine transport in plasma-membrane vesicles from rat liver. , 1978, The Biochemical journal.

[12]  B. Portmann,et al.  Lymphocyte cytotoxicity for isolated hepatocytes in alcoholic liver disease. , 1977, Gastroenterology.

[13]  D. Tuma,et al.  Reaction of acetaldehyde with human erythrocyte membrane proteins , 1977, FEBS letters.

[14]  M. Korsten,et al.  High blood acetaldehyde levels after ethanol administration. Difference between alcoholic and nonalcoholic subjects. , 1975, The New England journal of medicine.

[15]  R. Borch,et al.  Cyanohydridoborate anion as a selective reducing agent , 1971 .

[16]  D. Friend,et al.  HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS , 1969, The Journal of cell biology.

[17]  D. A. Bennett,et al.  Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices. , 1963, The Biochemical journal.

[18]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.