Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensors.

One goal of recent research on plasmonic nanoparticle-based sensors is maximizing nanoparticle sensitivity or shift of resonance peak wavelength per refractive index change. Equally important is a measurement system's peak location uncertainty or shift resolution. We provide systematic analyses and discuss optimization of factors that determine peak location uncertainty, reporting values as low as 0.3 nm for the presented scheme. This type of analysis is important, in part, because it provides a means of evaluating detection thresholds for biosensor applications such as analyte binding. We estimate thresholds of 310 streptavidin molecules for the presented scheme and 20 molecules with system improvements.

[1]  Adam Wax,et al.  Epi-illumination through the microscope objective applied to darkfield imaging and microspectroscopy of nanoparticle interaction with cells in culture. , 2006, Optics express.

[2]  Molly M. Miller,et al.  Sensitivity of metal nanoparticle surface plasmon resonance to the dielectric environment. , 2005, The journal of physical chemistry. B.

[3]  Carsten Sönnichsen,et al.  A molecular ruler based on plasmon coupling of single gold and silver nanoparticles , 2005, Nature Biotechnology.

[4]  Adam Wax,et al.  Substrate effect on refractive index dependence of plasmon resonance for individual silver nanoparticles observed using darkfield microspectroscopy. , 2005, Optics express.

[5]  Janos Vörös,et al.  The density and refractive index of adsorbing protein layers. , 2004, Biophysical journal.

[6]  Shuming Nie,et al.  Using Solution-Phase Nanoparticles, Surface-Confined Nanoparticle Arrays and Single Nanoparticles as Biological Sensing Platforms , 2004, Journal of Fluorescence.

[7]  W. P. Hall,et al.  A Localized Surface Plasmon Resonance Biosensor: First Steps toward an Assay for Alzheimer's Disease , 2004 .

[8]  George Chumanov,et al.  Measuring the Distance Dependence of the Local Electromagnetic Field from Silver Nanoparticles , 2004 .

[9]  Molly M. Miller,et al.  Controlling the Sensing Volume of Metal Nanosphere Molecular Sensors , 2004 .

[10]  Adam D. McFarland,et al.  Single Silver Nanoparticles as Real-Time Optical Sensors with Zeptomole Sensitivity , 2003 .

[11]  T. Klar,et al.  Biomolecular Recognition Based on Single Gold Nanoparticle Light Scattering , 2003 .

[12]  David R. Smith,et al.  Local Refractive Index Dependence of Plasmon Resonance Spectra from Individual Nanoparticles , 2003 .

[13]  Gregory V. Hartland,et al.  Heat Dissipation for Au Particles in Aqueous Solution: Relaxation Time versus Size , 2002 .

[14]  David R. Smith,et al.  Shape effects in plasmon resonance of individual colloidal silver nanoparticles , 2002 .

[15]  Hideki T. Miyazaki,et al.  Resonant light scattering from individual Ag nanoparticles and particle pairs , 2002 .

[16]  Ashutosh Chilkoti,et al.  A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. , 2002, Analytical chemistry.

[17]  T. Itoh,et al.  Direct Demonstration of Environment-Sensitive Surface Plasmon Resonance Band in Single Gold Nanoparticles , 2002 .

[18]  M. Levenson,et al.  Estimating the root mean square of a wave front and its uncertainty. , 2001, Applied optics.

[19]  N. Nath,et al.  Interfacial phase transition of an environmentally responsive elastin biopolymer adsorbed on functionalized gold nanoparticles studied by colloidal surface plasmon resonance. , 2001, Journal of the American Chemical Society.

[20]  Takayuki Okamoto,et al.  Near-field spectral analysis of metallic beads , 2001 .

[21]  Bernhard Lamprecht,et al.  Spectroscopy of single metallic nanoparticles using total internal reflection microscopy , 2000 .