Production technologies for monoclonal antibodies and their fragments.

In recent years, monoclonal antibodies have emerged as an increasingly important class of human therapeutics. A variety of forms of antibodies, including fragments such as Fabs, Fab'2s and single-chain Fvs, are also being evaluated for a range of different purposes. A variety of expression systems and improvements within these systems have been developed to address these growing and diverse needs.

[1]  M Angela Taipa,et al.  Antibodies and Genetically Engineered Related Molecules: Production and Purification , 2004, Biotechnology progress.

[2]  W. Miller,et al.  Selected amino acids protect hybridoma and CHO cells from elevated carbon dioxide and osmolality. , 2002, Biotechnology and bioengineering.

[3]  Roy Jefferis,et al.  Glycosylation of human IgG antibodies: Relevance to therapeutic applications , 2002 .

[4]  Mohamed Al-Rubeai,et al.  Uncoupling of cell growth and proliferation results in enhancement of productivity in p21CIP1‐arrested CHO cells , 2004, Biotechnology and bioengineering.

[5]  L. Simmons,et al.  Expression of full-length immunoglobulins in Escherichia coli: rapid and efficient production of aglycosylated antibodies. , 2002, Journal of immunological methods.

[6]  Christian M. Metallo,et al.  Serum-free suspension cultivation of PER.C6(R) cells and recombinant adenovirus production under different pH conditions. , 2002, Biotechnology and bioengineering.

[7]  E. Keshavarz‐Moore,et al.  Factors Affecting the Production of a Single‐Chain Antibody Fragment by Aspergillus awamoriin a Stirred Tank Reactor , 2001 .

[8]  S. Côté,et al.  Inducible expression of Bcl‐XL restricts apoptosis resistance to the antibody secretion phase in hybridoma cultures , 2002, Biotechnology and bioengineering.

[9]  M. Betenbaugh,et al.  Part II. Overexpression of bcl-2 family members enhances survival of mammalian cells in response to various culture insults. , 2000, Biotechnology and bioengineering.

[10]  Sónia Sá Santos,et al.  Cell Growth Arrest by Nucleotides, Nucleosides and Bases as a Tool for Improved Production of Recombinant Proteins , 2003, Biotechnology progress.

[11]  M. Fussenegger,et al.  p27Kip1-mediated controlled proliferation technology increases constitutive sICAM production in CHO-DUKX adapted for growth in suspension and serum-free media. , 2002, Biotechnology and bioengineering.

[12]  S. Sharfstein,et al.  Hyperosmotic Stress in Murine Hybridoma Cells: Effects on Antibody Transcription, Translation, Posttranslational Processing, and the Cell Cycle , 2008, Biotechnology progress.

[13]  T. Logtenberg,et al.  High‐Level Expression of Recombinant IgG in the Human Cell Line PER.C6 , 2003, Biotechnology progress.

[14]  J. Tso,et al.  Engineered Human IgG Antibodies with Longer Serum Half-lives in Primates* , 2004, Journal of Biological Chemistry.

[15]  L. Presta,et al.  Engineering therapeutic antibodies for improved function. , 2002, Biochemical Society transactions.

[16]  Humphreys Dp,et al.  Therapeutic antibody production technologies: molecules, applications, expression and purification. , 2001 .

[17]  Young Hwan Kim,et al.  Proteome Analysis of Antibody‐Expressing CHO Cells in Response to Hyperosmotic Pressure , 2003, Biotechnology progress.

[18]  Meng H. Heng,et al.  Characterization of Humanized Antibodies Secreted by Aspergillus niger , 2004, Applied and Environmental Microbiology.

[19]  A E Cunha,et al.  Methanol induction optimization for scFv antibody fragment production in Pichia pastoris , 2004, Biotechnology and bioengineering.

[20]  M. Betenbaugh,et al.  Study of caspase inhibitors for limiting death in mammalian cell culture. , 2003, Biotechnology and bioengineering.

[21]  O. Merten,et al.  Effect of serum-free and serum-containing medium on cellular levels of ER-based proteins in various mouse hybridoma cell lines. , 1997, Biotechnology and Bioengineering.

[22]  A. Lawson,et al.  A plasmid system for optimization of Fab' production in Escherichia coli: importance of balance of heavy chain and light chain synthesis. , 2002, Protein expression and purification.

[23]  K. Shitara,et al.  The Absence of Fucose but Not the Presence of Galactose or Bisecting N-Acetylglucosamine of Human IgG1 Complex-type Oligosaccharides Shows the Critical Role of Enhancing Antibody-dependent Cellular Cytotoxicity* , 2003, The Journal of Biological Chemistry.

[24]  L. Presta,et al.  Adapting pharmacokinetic properties of a humanized anti-interleukin-8 antibody for therapeutic applications using site-specific pegylation. , 2001, Cytokine.

[25]  Alan J Dickson,et al.  Stability of protein production from recombinant mammalian cells , 2003, Biotechnology and bioengineering.

[26]  G. Lee,et al.  Effect of doxycycline‐regulated calnexin and calreticulin expression on specific thrombopoietin productivity of recombinant chinese hamster ovary cells , 2004, Biotechnology and bioengineering.

[27]  A. P. Chapman,et al.  PEGylated antibodies and antibody fragments for improved therapy: a review. , 2002, Advanced drug delivery reviews.

[28]  Alastair D G Lawson,et al.  Co‐expression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab′ fragment expressed in Escherichia coli , 1996, FEBS letters.

[29]  W. Miller,et al.  Hyperosmotic Stress and Elevated pCO2 Alter Monoclonal Antibody Charge Distribution and Monosaccharide Content , 2002, Biotechnology progress.

[30]  L. Presta,et al.  Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human FcγRIII and Antibody-dependent Cellular Toxicity* , 2002, The Journal of Biological Chemistry.

[31]  Daniel I. C. Wang,et al.  A GFP-based screen for growth-arrested, recombinant protein-producing cells. , 2002, Biotechnology and bioengineering.

[32]  M. Fussenegger,et al.  Genetic optimization of recombinant glycoprotein production by mammalian cells. , 1999, Trends in biotechnology.

[33]  G. Lee,et al.  Development of apoptosis-resistant dihydrofolate reductase-deficient Chinese hamster ovary cell line. , 2003, Biotechnology and Bioengineering.

[34]  W. Miller,et al.  Characterization of hybridoma cell responses to elevated pCO(2) and osmolality: intracellular pH, cell size, apoptosis, and metabolism. , 2002, Biotechnology and bioengineering.

[35]  Sung Hyun Kim,et al.  Effect of Low Culture Temperature on Specific Productivity and Transcription Level of Anti‐4–1BB Antibody in Recombinant Chinese Hamster Ovary Cells , 2008, Biotechnology progress.

[36]  Mohamed Al-Rubeai,et al.  Modulation of Cell Cycle for Enhancement of Antibody Productivity in Perfusion Culture of NS0 Cells , 2003, Biotechnology progress.

[37]  M. Carrondo,et al.  Metabolic changes during cell growth inhibition by p27 overexpression , 2003, Applied Microbiology and Biotechnology.

[38]  G. Chiang,et al.  A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity. , 2003, Journal of immunological methods.

[39]  P. Lerouge,et al.  Galactose-extended glycans of antibodies produced by transgenic plants , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[40]  F. Gòdia,et al.  Decoupling cell growth and product formation in Chinese hamster ovary cells through metabolic control. , 2001, Biotechnology and bioengineering.

[41]  Julie Varley,et al.  The response of GS-NS0 myeloma cells to single and multiple pH perturbations. , 2002, Biotechnology and bioengineering.

[42]  F. Dorner,et al.  Higher expression of fab antibody fragments in a CHO cell line at reduced temperature , 2003, Biotechnology and bioengineering.

[43]  Mariangela Spitali,et al.  Therapeutic antibody fragments with prolonged in vivo half-lives , 1999, Nature Biotechnology.

[44]  Min Zhang,et al.  Albumin Binding as a General Strategy for Improving the Pharmacokinetics of Proteins* , 2002, The Journal of Biological Chemistry.

[45]  M. Betenbaugh,et al.  Aven and Bcl‐xL enhance protection against apoptosis for mammalian cells exposed to various culture conditions , 2004, Biotechnology and bioengineering.

[46]  D. Andersen,et al.  Recombinant protein expression for therapeutic applications. , 2002, Current opinion in biotechnology.

[47]  A. Morris,et al.  EASE Vectors for Rapid Stable Expression of Recombinant Antibodies , 2003, Biotechnology progress.

[48]  Michael J Gramer,et al.  Antibody production by a hybridoma cell line at high cell density is limited by two independent mechanisms. , 2002, Biotechnology and bioengineering.

[49]  John C Joly,et al.  High‐level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple‐mutant (degP prc spr) host strain , 2004, Biotechnology and bioengineering.

[50]  J. McGrew,et al.  High‐level expression of full‐length antibodies using trans‐complementing expression vectors , 2003, Biotechnology and bioengineering.

[51]  G. Lee,et al.  Inhibition of sodium butyrate-induced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of caspase-3. , 2002, Biotechnology and bioengineering.

[52]  Martin Fussenegger,et al.  Impact of coexpression and coamplification of sICAM and antiapoptosis determinants bcl-2/bcl-x(L) on productivity, cell survival, and mitochondria number in CHO-DG44 grown in suspension and serum-free media. , 2002, Biotechnology and bioengineering.

[53]  H. Katinger,et al.  Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting. , 2000, Biotechnology and bioengineering.

[54]  G. Lee,et al.  Effects of Cloned Gene Dosage on the Response of Recombinant CHO Cells to Hyperosmotic Pressure in Regard to Cell Growth and Antibody Production , 2001, Biotechnology progress.

[55]  M. Ayala,et al.  Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris. , 2000, Journal of biotechnology.

[56]  A. Plückthun,et al.  The Periplasmic Escherichia coli Peptidylprolyl cis,trans-Isomerase FkpA , 2000, The Journal of Biological Chemistry.

[57]  S. R. Fox,et al.  Maximizing interferon‐γ production by chinese hamster ovary cells through temperature shift optimization: Experimental and modeling , 2004, Biotechnology and bioengineering.

[58]  E. Hood,et al.  Monoclonal antibody manufacturing in transgenic plants--myths and realities. , 2002, Current opinion in biotechnology.

[59]  J. Field,et al.  Riboflavin- and cobalamin-mediated biodegradation of chloroform in a methanogenic consortium. , 2005, Biotechnology and bioengineering.

[60]  G. Lee,et al.  Key Determinants in the Occurrence of Clonal Variation in Humanized Antibody Expression of CHO Cells during Dihydrofolate Reductase Mediated Gene Amplification , 2001, Biotechnology progress.

[61]  H. Katinger,et al.  Enhancement of Monoclonal Antibody Production by Lysine‐Containing Peptides , 2003, Biotechnology progress.

[62]  David P Humphreys.  Production of antibodies and antibody fragments in Escherichia coli and a comparison of their functions, uses and modification. , 2003, Current opinion in drug discovery & development.

[63]  S L Morrison,et al.  Effect of glycosylation on antibody function: implications for genetic engineering. , 1997, Trends in biotechnology.

[64]  Teresa Mitchell,et al.  Production of Complex Human Glycoproteins in Yeast , 2003, Science.

[65]  Noelle-Ann Sunstrom,et al.  High-throughput clonal selection of recombinant CHO cells using a dominant selectable and amplifiable metallothionein-GFP fusion protein. , 2002, Biotechnology and bioengineering.

[66]  Uwe Kärst,et al.  Effects of buffering conditions and culture pH on production rates and glycosylation of clinical phase I anti-melanoma mouse IgG3 monoclonal antibody R24. , 2003, Biotechnology and bioengineering.

[67]  Z. Darieva,et al.  Transfection of NS0 myeloma fusion partner cells with HSP70 gene results in higher hybridoma yield by improving cellular resistance to apoptosis. , 2003, Biotechnology and bioengineering.

[68]  Gyun Min Lee,et al.  Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. , 2003, Biotechnology and bioengineering.