The roles and function of cellulose-binding domains

Most cellulolytic enzymes consist of distinct catalytic and cellulose-binding domains (CBDs). Similar domain structures are also found in enzymes degrading other insoluble carbohydrates such as raw starch and chitin. Such binding domains improve the binding and facilitate the activity of the catalytic domain on the insoluble but not on soluble substrates. Based on their amino acid sequence similarities, the CBDs have been divided into several different families. Structure determination and subsequent mutagenesis studies have revealed that CBDs rely on several aromatic amino acids for binding to the cellulose surfaces. The CBDs binding to crystalline cellulose have different topologies but share similar rigid backbone structures for correct positioning of the side chains required for the substrate recognition and binding. CBDs represent ideal affinity tags for specific immobilisation of various other proteins to cellulose. Furthermore, improved understanding and control of their action will be important for the improvement of the biotechnological value of cellulolytic enzymes.

[1]  D. Kilburn,et al.  The nature of the cellulose‐binding domain effects the activities of a bacterial endoglucanase on different forms of cellulose , 1993 .

[2]  D. Kilburn,et al.  Enhancement of the Endo-β-1,4-glucanase Activity of an Exocellobiohydrolase by Deletion of a Surface Loop (*) , 1995, The Journal of Biological Chemistry.

[3]  H. Gilbert,et al.  A modular xylanase containing a novel non-catalytic xylan-specific binding domain. , 1995, The Biochemical journal.

[4]  N. Vyas.  Atomic features of protein-carbohydrate interactions , 1991 .

[5]  M. Wilchek,et al.  Cellulosome domains for novel biotechnological application , 1995 .

[6]  E Setter,et al.  Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum , 1983, Journal of bacteriology.

[7]  T. Reinikainen,et al.  Comparison of the adsorption properties of a single-chain antibody fragment fused to a fungal or bacterial cellulose-binding domain , 1997 .

[8]  M. Penttilä,et al.  Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain , 1995, Applied and environmental microbiology.

[9]  M. Fletcher.  Bacterial adhesion : molecular and ecological diversity , 1996 .

[10]  I. H. Segel,et al.  Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A , 1993, Journal of bacteriology.

[11]  P. Gans,et al.  Overproduction, purification and characterization of the cellulose-binding domain of the Erwinia chrysanthemi secreted endoglucanase EGZ. , 1995, European journal of biochemistry.

[12]  J. Greenwood,et al.  The cellulose-binding domains of cellulases: tools for biotechnology , 1989 .

[13]  Robin F. B. Turner,et al.  Technology for Regenerable Biosensor Probes Based on Enzyme‐Cellulose Binding Domain Conjugates , 1994 .

[14]  J. Ståhlberg,et al.  Effect of potential binding site overlap to binding of cellulose to cellulose: a two‐dimensional simulation , 1996, FEBS letters.

[15]  D. Wilson,et al.  Disulfide arrangement and functional domains of beta-1,4-endoglucanse E5 from Thermomonospora fusca. , 1993, Biochemistry.

[16]  J. Buchert,et al.  Modification of hardwood dissolving pulp with purifiedTrichoderma reesei cellulases , 1996 .

[17]  T. Reinikainen,et al.  Effects of pH and high ionic strength on the adsorption and activity of native and mutated cellobiohydrolase I from Trichoderma reesei , 1995, Proteins.

[18]  J. Hall,et al.  Spatial separation of protein domains is not necessary for catalytic activity or substrate binding in a xylanase. , 1990, The Biochemical journal.

[19]  D. Kilburn,et al.  A Bifunctional Affinity Linker to Couple Antibodies to Cellulose , 1993, Bio/Technology.

[20]  H. Gilbert,et al.  The Conserved Noncatalytic 40-Residue Sequence in Cellulases and Hemicellulases from Anaerobic Fungi Functions as a Protein Docking Domain (*) , 1995, The Journal of Biological Chemistry.

[21]  J. Ståhlberg,et al.  A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I , 1991, Bio/Technology.

[22]  B. Henrissat,et al.  Visualization of the adsorption of a bacterial endo-beta-1,4-glucanase and its isolated cellulose-binding domain to crystalline cellulose. , 1993, International journal of biological macromolecules.

[23]  D. Kilburn,et al.  Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis. , 1988, The Journal of biological chemistry.

[24]  Christos Stathopoulos,et al.  Specific Adhesion and Hydrolysis of Cellulose by Intact Escherichia coli Expressing Surface Anchored Cellulase or Cellulose Binding Domains , 1993, Bio/Technology.

[25]  D. Kilburn,et al.  Enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein. , 1991, Enzyme and microbial technology.

[26]  P. Birch,et al.  Substrate-dependent differential splicing of introns in the regions encoding the cellulose binding domains of two exocellobiohydrolase I-like genes in Phanerochaete chrysosporium , 1995, Applied and environmental microbiology.

[27]  C. Haynes,et al.  Interaction of polysaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 1. Binding specificity and calorimetric analysis. , 1996, Biochemistry.

[28]  J. Greenwood,et al.  Purification of human interleukin-2 using the cellulose-binding domain of a prokaryotic cellulase. , 1995, Bioseparation (Dordrecht).

[29]  A. Klyosov,et al.  Trends in biochemistry and enzymology of cellulose degradation. , 1990, Biochemistry.

[30]  P. Kraulis,et al.  Investigation of the function of mutated cellulose‐binding domains of Trichoderma reesei cellobiohydrolase I , 1992, Proteins.

[31]  W. Steiner,et al.  Cellulose hydrolysis by the cellulases from Trichoderma reesei: a new model for synergistic interaction. , 1994, The Biochemical journal.

[32]  M. Wilchek,et al.  Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum , 1995, Applied and environmental microbiology.

[33]  H. van Tilbeurgh,et al.  Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei , 1986 .

[34]  C. Haynes,et al.  Binding of the cellulose-binding domain of exoglucanase Cex from Cellulomonas fimi to insoluble microcrystalline cellulose is entropically driven. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[35]  M. Penttilä,et al.  Acetyl xylan esterase from Trichoderma reesei contains an active-site serine residue and a cellulose-binding domain. , 1996, European journal of biochemistry.

[36]  T. Boller,et al.  The N-Terminal Cysteine-Rich Domain of Tobacco Class I Chitinase Is Essential for Chitin Binding but Not for Catalytic or Antifungal Activity , 1993, Plant physiology.

[37]  T. Teeri,et al.  The cellulose-binding domain of the major cellobiohydrolase of Trichoderma reesei exhibits true reversibility and a high exchange rate on crystalline cellulose. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[38]  L. Ruohonen,et al.  Cello-oligosaccharide hydrolysis by cellobiohydrolase II from Trichoderma reesei. Association and rate constants derived from an analysis of progress curves. , 1996, European journal of biochemistry.

[39]  Binding reversibility and surface exchange of Thermomonospora fusca E3 and E5 and Trichoderma reesei CBHI , 1997 .

[40]  G. Lindeberg,et al.  The difference in affinity between two fungal cellulose‐binding domains is dominated by a single amino acid substitution , 1995, FEBS letters.

[41]  T. Reinikainen,et al.  Domain function in Trichoderma reesei cellobiohydrolases , 1992 .

[42]  L. Ruohonen,et al.  Characterization of a Double Cellulose-binding Domain , 1996, The Journal of Biological Chemistry.

[43]  H. Jespersen,et al.  Comparison of the domain-level organization of starch hydrolases and related enzymes. , 1991, The Biochemical journal.

[44]  J. Ståhlberg,et al.  A binding-site-deficient, catalytically active, core protein of endoglucanase III from the culture filtrate of Trichoderma reesei. , 1988, European journal of biochemistry.

[45]  B. Henrissat,et al.  Colloidal gold labelling of l,4‐β‐D‐glucan cellobiohydrolase adsorbed on cellulose substrates , 1984 .

[46]  P. Coutinho,et al.  Structure-function relationships in the catalytic and starch binding domains of glucoamylase. , 1994, Protein engineering.

[47]  D. Kilburn,et al.  C1-Cx revisited: intramolecular synergism in a cellulase. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[48]  T. Teeri,et al.  The Cellulases Endoglucanase I and Cellobiohydrolase II of Trichoderma reesei Act Synergistically To Solubilize Native Cotton Cellulose but Not To Decrease Its Molecular Size , 1996, Applied and environmental microbiology.

[49]  J. Greenwood,et al.  Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose , 1989, FEBS letters.

[50]  L. McIntosh,et al.  Probing the role of tryptophan residues in a cellulose‐binding domain by chemical modification , 1996, Protein science : a publication of the Protein Society.

[51]  R. Warren.  Microbial hydrolysis of polysaccharides. , 1996, Annual review of microbiology.

[52]  Bernard Henrissat,et al.  Cellulases and their interaction with cellulose , 1994 .

[53]  M. Claeyssens,et al.  Domain structure of cellobiohydrolase II as studied by small angle X-ray scattering: close resemblance to cellobiohydrolase I. , 1988, Biochemical and biophysical research communications.

[54]  D. Kilburn,et al.  Bacterial cellulose-binding domain-like sequences in eucaryotic polypeptides. , 1991, Protein sequences & data analysis.

[55]  M. Penttilä,et al.  EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme. , 1988, Gene.

[56]  D. Wilson,et al.  Characterization and sequence of a Thermomonospora fusca xylanase , 1994, Applied and environmental microbiology.

[57]  T. Wood,et al.  METHODS FOR MEASURING CELLULASE ACTIVITIES , 1988 .

[58]  W. Liebl,et al.  Identification of a novel cellulose‐binding domain the multidomain 120 kDa xylanase XynA of the hyperthermophilic bacterium Thermotoga maritima , 1995 .

[59]  R. Turner,et al.  Production and properties of a bifunctional fusion protein that mediates attachment of vero cells to cellulosic matrices , 1995, Biotechnology and bioengineering.

[60]  D. Kilburn,et al.  Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains , 1987, FEBS letters.

[61]  B. Henrissat,et al.  The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose. , 1992, The Journal of biological chemistry.

[62]  T. Joyce,et al.  Enzyme activity recovery from secondary fiber treated with cellulase and xylanase , 1996 .

[63]  H. O. D. op den Camp,et al.  Adsorption characteristics of cellulolytic enzymes from the anaerobic fungus Piromyces sp. strain E2 on microcrystalline cellulose , 1996, Applied and environmental microbiology.

[64]  P. Kraulis.  A program to produce both detailed and schematic plots of protein structures , 1991 .

[65]  J. Ståhlberg,et al.  Trichoderma reesei has no true exo-cellulase: all intact and truncated cellulases produce new reducing end groups on cellulose. , 1993, Biochimica et biophysica acta.

[66]  L. McIntosh,et al.  Cellulose‐binding Domains , 1996 .

[67]  M. Penttilä,et al.  Role of the interdomain linker peptide of Trichoderma reesei cellobiohydrolase I in its interaction with crystalline cellulose. , 1993, The Journal of biological chemistry.

[68]  J. Vandekerckhove,et al.  Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis. , 1988, European journal of biochemistry.

[69]  Tuula T. Teeri,et al.  Cellulase families and their genes , 1987 .

[70]  D. Hon.  Cellulose: a random walk along its historical path , 1994 .

[71]  T. Reinikainen,et al.  The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei. , 1994, Science.

[72]  H. Chanzy.  Aspects of cellulose structure , 1990 .

[73]  A. Annila,et al.  Identification of functionally important amino acids in the cellulose‐binding domain of Trichoderma reesei cellobiohydrolase I , 1995, Protein science : a publication of the Protein Society.

[74]  J. Beintema.  Structural features of plant chitinases and chitin‐binding proteins , 1994, FEBS letters.

[75]  P. Karplus,et al.  Crystal structure of the catalytic domain of a thermophilic endocellulase. , 1993, Biochemistry.

[76]  J. Saddler,et al.  Enzymatic Separation of High–Quality Uninked Pulp Fibers from Recycled Newspaper , 1994, Bio/Technology.

[77]  D. Kilburn,et al.  Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain , 1995, Journal of bacteriology.

[78]  Tuula T. Teeri,et al.  Crystalline cellulose degradation : new insight into the function of cellobiohydrolases , 1997 .

[79]  W. M. Hosny,et al.  Metal chelates with some cellulose derivatives; part IV. Structural chemistry of HEC complexes , 1996 .

[80]  F. Quiocho.  Probing the atomic interactions between proteins and carbohydrates. , 1993, Biochemical Society transactions.

[81]  P. Kraulis,et al.  Determination of the three-dimensional solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. A study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing. , 1989, Biochemistry.

[82]  R. Doi,et al.  The Clostridium cellulovorans cellulosome. , 1994, Critical reviews in microbiology.

[83]  J. Hall,et al.  The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel. , 1995, The Biochemical journal.

[84]  D. Kilburn,et al.  The cellulose‐binding domain (CBDCex) of an exoglucanase from Cellulomonas fimi: Production in Escherichia coli and characterization of the polypeptide , 1993, Biotechnology and bioengineering.

[85]  G. Xue,et al.  Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Trichoderma reesei cellobiohydrolase II , 1996, Applied and environmental microbiology.

[86]  L. Kay,et al.  Solution structure of a cellulose-binding domain from Cellulomonas fimi by nuclear magnetic resonance spectroscopy , 1995 .

[87]  F. Rombouts,et al.  Adsorption and kinetic behavior of purified endoglucanases and exoglucanases from Trichoderma viride , 1987, Biotechnology and bioengineering.

[88]  Liisa Viikari,et al.  Effects of purified Trichoderma reesei cellulases on the fiber properties of kraft pulp , 1995 .

[89]  B. Henrissat,et al.  Evidence for a general role for high‐affinity non‐catalytic cellulose binding domains in microbial plant ceil wall hydroiases , 1994, Molecular microbiology.

[90]  M. D. Joshi,et al.  Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by nuclear magnetic resonance spectroscopy. , 1996, Biochemistry.

[91]  B. Henrissat,et al.  Structures and mechanisms of glycosyl hydrolases. , 1995, Structure.

[92]  K. Sakka,et al.  Nucleotide sequence of the Clostridium stercorarium xynA gene encoding xylanase A: identification of catalytic and cellulose binding domains. , 1993, Bioscience, biotechnology, and biochemistry.

[93]  T. Steitz,et al.  Crystal structure of a bacterial family‐III cellulose‐binding domain: a general mechanism for attachment to cellulose. , 1996, The EMBO journal.

[94]  B. Svensson,et al.  Site-directed mutagenesis of histidine 93, aspartic acid 180, glutamic acid 205, histidine 290, and aspartic acid 291 at the active site and tryptophan 279 at the raw starch binding site in barley alpha-amylase 1. , 1993, The Journal of biological chemistry.

[95]  J. Knowles,et al.  Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. , 1990, Science.

[96]  D. Kilburn,et al.  The cellulose‐binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding , 1994, Molecular microbiology.

[97]  T. Teeri,et al.  Molecular dynamics simulation of fungal cellulose-binding domains: differences in molecular rigidity but a preserved cellulose binding surface. , 1995, Protein engineering.

[98]  H. Schrempf,et al.  Binding and substrate specificities of a Streptomyces olivaceoviridis chitinase in comparison with its proteolytically processed form. , 1995, European journal of biochemistry.

[99]  E. Bayer,et al.  The cellulosome--a treasure-trove for biotechnology. , 1994, Trends in biotechnology.

[100]  H. Gilbert,et al.  The role of conserved tryptophan residues in the interaction of a bacterial cellulose binding domain with its ligand. , 1993, FEMS microbiology letters.

[101]  T. Watanabe,et al.  Structure of the gene encoding chitinase D of Bacillus circulans WL-12 and possible homology of the enzyme to other prokaryotic chitinases and class III plant chitinases , 1992, Journal of bacteriology.

[102]  J. Aubert,et al.  The biological degradation of cellulose. , 1994, FEMS microbiology reviews.

[103]  D. Kilburn,et al.  Cellulose-binding domains : classification and properties , 1995 .

[104]  D. Kilburn,et al.  Deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase A (CenA) of Cellulomonas fimi alters its conformation and catalytic activity. , 1991, The Journal of biological chemistry.

[105]  B. Henrissat,et al.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. , 1991, Microbiological reviews.

[106]  D. Kilburn,et al.  The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N‐terminal repeats , 1992, Molecular microbiology.

[107]  Thomas W. Jeffries,et al.  Roles for microbial enzymes in pulp and paper processing , 1996 .

[108]  S. Kauppinen,et al.  Homologous domains in Trichoderma reesei cellulolytic enzymes: gene sequence and expression of cellobiohydrolase II. , 1987, Gene.

[109]  N. Gilkes,et al.  Changes in the molecular-size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases. , 1994, The Biochemical journal.

[110]  N. Sharon,et al.  Lectins--proteins with a sweet tooth: functions in cell recognition. , 1995, Essays in biochemistry.

[111]  A. Demain,et al.  The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome. , 1995, Proceedings of the National Academy of Sciences of the United States of America.