Double-Fluorescent-Labeled Single-Chain Antibodies Showing Antigen-Dependent Fluorescence Ratio Change

Fluorescence ratio probes are useful tools for quantitative detection of target molecules even if the concentration of probe molecules is unknown. However, a general and widely applicable method fo...

[1]  H. Ueda,et al.  Open flower fluoroimmunoassay: a general method to make fluorescent protein-based immunosensor probes. , 2015, Analytical chemistry.

[2]  H. Ueda,et al.  From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins. , 2014, Biochimica et biophysica acta.

[3]  Laurens Lindenburg,et al.  Engineering Genetically Encoded FRET Sensors , 2014, Sensors.

[4]  Hiroshi Ueda,et al.  Strategy for Making a Superior Quenchbody to Proteins: Effect of the Fluorophore Position , 2014, Sensors.

[5]  Ryoji Abe,et al.  Ultra Q-bodies: quench-based antibody probes that utilize dye-dye interactions with enhanced antigen-dependent fluorescence , 2014, Scientific Reports.

[6]  Masaki Inagaki,et al.  Detection of vimentin serine phosphorylation by multicolor Quenchbodies. , 2013, Biosensors & bioelectronics.

[7]  K. Johnsson,et al.  Switchable fluorophores for protein labeling in living cells. , 2011, Current opinion in chemical biology.

[8]  Takahiro Hohsaka,et al.  "Quenchbodies": quench-based antibody probes that show antigen-dependent fluorescence. , 2011, Journal of the American Chemical Society.

[9]  Steven M. Lewis,et al.  A biosensor generated via high throughput screening quantifies cell edge Src dynamics , 2011, Nature chemical biology.

[10]  S. Ebisu,et al.  Incorporation of fluorescent non-natural amino acids into N-terminal tag of proteins in cell-free translation and its dependence on position and neighboring codons. , 2010, Journal of bioscience and bioengineering.

[11]  Shohei Koide,et al.  Rational conversion of affinity reagents into label-free sensors for Peptide motifs by designed allostery. , 2010, ACS chemical biology.

[12]  J. Hall,et al.  Applications of single-chain variable fragment antibodies in therapeutics and diagnostics. , 2009, Biotechnology advances.

[13]  T. Hohsaka,et al.  Position‐Specific Incorporation of Fluorescent Non‐natural Amino Acids into Maltose‐Binding Protein for Detection of Ligand Binding by FRET and Fluorescence Quenching , 2009, Chembiochem : a European journal of chemical biology.

[14]  K. Tan,et al.  Semisynthetic fluorescent sensor proteins based on self-labeling protein tags. , 2009, Journal of the American Chemical Society.

[15]  P. Scheerer,et al.  The structure of the anti‐c‐myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops , 2008, Proteins.

[16]  K. Kojima,et al.  Comprehensive screening of amber suppressor tRNAs suitable for incorporation of non-natural amino acids in a cell-free translation system. , 2008, Biochemical and biophysical research communications.

[17]  Sandra Lynch,et al.  An scFv intrabody against the nonamyloid component of alpha-synuclein reduces intracellular aggregation and toxicity. , 2008, Journal of molecular biology.

[18]  M. Sisido,et al.  FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids , 2006, Nature Methods.

[19]  Kevin Truong,et al.  Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics , 2006, Biotechnology Letters.

[20]  Igor L. Medintz,et al.  Maltose-binding protein: a versatile platform for prototyping biosensing. , 2006, Current opinion in biotechnology.

[21]  H. Hellinga,et al.  Periplasmic binding proteins: a versatile superfamily for protein engineering. , 2004, Current opinion in structural biology.

[22]  A. Miyawaki.  Visualization of the spatial and temporal dynamics of intracellular signaling. , 2003, Developmental cell.

[23]  M. Sisido,et al.  Efficient Incorporation of Nonnatural Amino Acids with Large Aromatic Groups into Streptavidin in In Vitro Protein Synthesizing Systems , 1999 .