Blood contamination and the measurement of salivary progesterone and estradiol

The impact of blood leakage due to microinjury to the oral cavity on the measurement of salivary reproductive hormones was examined. Saliva samples were collected before, immediately after, and then every 15 min for 1 h following vigorous tooth brushing. Blood in saliva was quantified by visual inspection of discoloration and an immunoassay for transferrin. Levels of progesterone increased, and levels of estradiol decreased, in saliva after microinjury. These changes were present only immediately after microinjury. The findings have implications for the use of salivary assays in biobehavioral research, short-term dynamic investigations, pharmacokinetic analyses, and studies of chronobiological changes in progesterone and estradiol levels.

[1]  W. O'brien,et al.  A meta-analysis of the effect of hormone replacement therapy upon depressed mood , 1997, Psychoneuroendocrinology.

[2]  G. Bentley,et al.  Salivary progesterone levels and rate of ovulation are significantly lower in poorer than in better-off urban-dwelling Bolivian women. , 2002, Human reproduction.

[3]  P. Ellison,et al.  Comparison of salivary steroid profiles in naturally occurring conception and non-conception cycles. , 1996, Human reproduction.

[4]  M. Seeman.  Psychopathology in women and men: focus on female hormones. , 1997, The American journal of psychiatry.

[5]  G. Gilbert,et al.  Racial and socioeconomic disparities in oral disadvantage, a measure of oral health-related quality of life: 24-month incidence. , 2002, Journal of public health dentistry.

[6]  M. Gunnar,et al.  Assessing salivary cortisol in studies of child development. , 1998, Child development.

[7]  T. Arendorf,et al.  Oral manifestations of HIV infection in 600 South African patients. , 2007, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[8]  E. Shirtcliff,et al.  Use of salivary biomarkers in biobehavioral research: cotton-based sample collection methods can interfere with salivary immunoassay results , 2001, Psychoneuroendocrinology.

[9]  P. Ellison.  Measurements of Salivary Progesterone a , 1993, Annals of the New York Academy of Sciences.

[10]  Vincent Nelson,et al.  Quantifying blood leakage into the oral mucosa and its effects on the measurement of cortisol, dehydroepiandrosterone, and testosterone in saliva , 2004, Hormones and Behavior.

[11]  Alan Booth,et al.  Salivary Testosterone Determination in Studies of Child Health and Development , 1999, Hormones and Behavior.

[12]  E. Shirtcliff,et al.  Assessing Estradiol in Biobehavioral Studies Using Saliva and Blood Spots: Simple Radioimmunoassay Protocols, Reliability, and Comparative Validity , 2000, Hormones and Behavior.

[13]  K. Sengoku,et al.  The Clinical Usefulness of Salivary Progesterone Measurement for the Evaluation of the Corpus luteum Function , 2002, Gynecologic and Obstetric Investigation.

[14]  C. Worthman,et al.  Sensitive salivary estradiol assay for monitoring ovarian function. , 1990, Clinical chemistry.

[15]  B. McEwen.  The Molecular and Neuroanatomical Basis for Estrogen Effects in the Central Nervous System , 1999 .

[16]  P. Gann,et al.  Saliva as a medium for investigating intra- and interindividual differences in sex hormone levels in premenopausal women. , 2001, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[17]  L. Cédard,et al.  Salivary oestradiol in spontaneous and stimulated menstrual cycles. , 1989, Human reproduction.

[18]  G R Bentley,et al.  Salivary estradiol and progesterone levels in conception and nonconception cycles in women: evaluation of a new assay for salivary estradiol. , 1999, Fertility and sterility.