Deletion Map of the Escherichia coli K-12 Sex Factor F: the Order of Eleven Transfer Cistrons

A series of Hfr deletion mutants was isolated. These mutants contain deletions which extend from a λ prophage into an Flac which is integrated into the gal operon. Transfer-deficient deletion mutants were found to fall into four different phenotypic groups when tested for male- and female-specific phage resistance. Conjugational and transductional complementation tests with Flac point mutants deficient in transfer (tra−) were performed, and the order of 11 tra cistrons was determined. The tra genes are all located between an F gene for the inhibition of female-specific phages and the transposed lac operon originally carried by the Flac. The order of genes in the Hfr studied was established to be: proC... φIIR... traJ traA traE traK traB traC traF traH traG traD traI...lac...attλ...bio.

[1]  M. Achtman,et al.  Conjugational Complementation Analysis of Transfer-Deficient Mutants of Flac in Escherichia coli , 1972, Journal of bacteriology.

[2]  M. Achtman,et al.  Genetic Analysis of Transfer by the Escherichia coli Sex Factor F, Using P1 Transductional Complementation , 1972, Journal of bacteriology.

[3]  J. Shapiro,et al.  Transposition of the lac Region to the gal Region of the Escherichia coli Chromosome: Isolation of λlac Transducing Bacteriophages , 1971, Journal of bacteriology.

[4]  M Achtman,et al.  A semi-automatic technique for conducting many bacterial matings concurrently. , 1971, Genetical research.

[5]  M. Achtman,et al.  Beginning a Genetic Analysis of Conjugational Transfer Determined by the F Factor in Escherichia coli by Isolation and Characterization of Transfer-Deficient Mutants , 1971, Journal of bacteriology.

[6]  N. Willetts.  Plasmid specificity of two proteins required for conjugation in E. coli K12. , 1971, Nature: New biology.

[7]  D. Finnegan,et al.  Characteristics of E. coli K12 strains carrying both an F prime and an R factor. , 1970, Genetical research.

[8]  Y. Hirota,et al.  Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis. , 1970, Genetics.

[9]  E. Otsubo.  Transfer-defective mutants of sex factors in Escherichia coli. II. Deletion mutants of an F-prime and deletion mapping of cistrons involved in genetic transfer. , 1970, Genetics.

[10]  J. Shapiro,et al.  The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity. , 1969, Genetics.

[11]  J. Beckwith,et al.  Allelic amber and ochre suppressors. , 1968, Journal of molecular biology.

[12]  R. Valentine,et al.  Sex hair (F-pili) mutants of E. coli. , 1967, Biochemical and biophysical research communications.

[13]  T. Watanabe,et al.  NEW TYPE OF SEX FACTOR-SPECIFIC BACTERIOPHAGE OF ESCHERICHIA COLI , 1964, Journal of bacteriology.

[14]  J. Schell,et al.  The restriction of phage T3 by certain strains of Escherichia coli , 1963 .

[15]  G. Maccacaro,et al.  Sex-Specific Bacteriophages of Escherichia coli K12. , 1961 .