Oxidative stability in grinds from omega-3 enhanced pork

Juárez, M., Dugan, M. E. R., Larsen, I. L., Thacker, R., Rolland, D. C. and Aalhus, J. L. 2011. Oxidative stability in grinds from omega-3 enhanced pork. Can. J. Anim. Sci. 91: 623-634. In order to investigate the effect of enhancing n-3 fatty acid levels in carcasses on the retail and sensory characteristics of pork grinds, as well as the ability of increasing dietary levels of vitamin E to stabilize the increase in PUFA levels from flaxseed supplementation, 81 pigs were used in a 3×3 factorial experiment. Dietary treatments included three lengths of time (0, 3 and 6 wk) of flaxseed supplementation and three levels of vitamin E (40, 200 and 400 mg kg feed-1). α-Tocopherol tissue levels increased with dietary vitamin E supplementation (P<0.001), and fat softness (P=0.018) and total PUFA and n-3 fatty acids (P<0.001) increased with flaxseed supplementation. Most other animal performance, carcass and lean pork quality traits were not affected (P>0.05) by dietary treatments. In ground pork, the increase in TBARS after 6 d in retail was lower (P<0.001) with increasing dietary vitamin E levels. However, vitamin E had no effect (P>0.05) on the higher (P<0.001) TBARS levels observed in grinds from flaxseed fed pigs. The dietary addition of flaxseed negatively modified (P<0.05) all the sensory attributes of both cooked and reheated products. Increasing the fat content of the grinds up to 30% seemed to counteract (P>0.05) the negative effects of feeding flaxseed for 3 wk. Feeding vitamin E was not able to prevent negative effects (P<0.05) observed during retail display in grinds with either increasing fat content or higher n-3 fatty acids from dietary flaxseed. However, increasing dietary vitamin E only showed a protective effect (P<0.05) on retail appearance after long periods of ageing.

[1]  R. Zijlstra,et al.  Increasing omega-3 levels through dietary co-extruded flaxseed supplementation negatively affects pork palatability. , 2011, Food chemistry.

[2]  L. Bünger,et al.  Meta-analysis of the effects of dietary vitamin E supplementation on α-tocopherol concentration and lipid oxidation in pork. , 2011, Meat science.

[3]  Qun Sun,et al.  Myoglobin and lipid oxidation interactions: mechanistic bases and control. , 2010, Meat science.

[4]  S. Lonergan,et al.  Biochemistry of postmortem muscle - lessons on mechanisms of meat tenderization. , 2010, Meat science.

[5]  R. Zijlstra,et al.  Feeding co-extruded flaxseed to pigs: effects of duration and feeding level on growth performance and backfat fatty acid composition of grower-finisher pigs. , 2010, Meat science.

[6]  M. S. Brewer,et al.  Effect of different dietary levels of natural-source vitamin E in grow-finish pigs on pork quality and shelf life. , 2009, Meat science.

[7]  Jian Peng,et al.  Duration of dietary linseed feeding affects the intramuscular fat, muscle mass and fatty acid composition in pig muscle , 2008 .

[8]  E. Webb,et al.  The animal fat paradox and meat quality. , 2008, Meat science.

[9]  J. Mourot,et al.  Influence of extruded linseed on growth, carcass composition, and meat quality of slaughtered pigs at one hundred ten and one hundred sixty kilograms of liveweight. , 2008, Journal of animal science.

[10]  D. Fremaut,et al.  Fatty acid profile and oxidative stability of pork as influenced by duration and time of dietary linseed or fish oil supplementation. , 2008, Journal of animal science.

[11]  W. M. Robertson,et al.  Comparing Subcutaneous Adipose Tissue in Beef and Muskox with Emphasis on trans 18:1 and Conjugated Linoleic Acids , 2007, Lipids.

[12]  A. Hugo,et al.  Significance of Porcine Fat Quality in Meat Technology: A Review , 2007 .

[13]  M. Popa,et al.  Effect of vitamin E in swine diet on animal growth performance and meat quality parameters. , 2007 .

[14]  D. Gerrard,et al.  Effect of dietary vitamin E supplementation and feeding period on pork quality. , 2006, Journal of animal science.

[15]  D. Gerrard,et al.  Effects of dietary vitamin E and fat supplementation on pork quality. , 2006, Journal of animal science.

[16]  P. Thacker,et al.  Performance and carcass characteristics of growing-finishing pigs fed barley-based diets supplemented with Linpro (extruded whole flaxseed and peas) or soybean meal , 2004 .

[17]  I. Cambero,et al.  Physicochemical characteristics of an α-linolenic acid and α-tocopherol-enriched cooked ham , 2004 .

[18]  C. Ruxton,et al.  The health benefits of omega-3 polyunsaturated fatty acids: a review of the evidence. , 2004, Journal of human nutrition and dietetics : the official journal of the British Dietetic Association.

[19]  C. Wenk,et al.  Influence of dietary vitamin E and C supplementation on vitamin E and C content and thiobarbituric acid reactive substances (TBARS) in different tissues of growing pigs , 2004, Archives of animal nutrition.

[20]  S. Smet,et al.  Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review , 2004 .

[21]  M. C. Lanari,et al.  Simultaneous determination of vitamin E homologs in chicken meat by liquid chromatography with fluorescence detection. , 2004, Journal of chromatography. A.

[22]  A. Fisher,et al.  Effects of fatty acids on meat quality: a review. , 2004, Meat science.

[23]  M. Cambero,et al.  Effect of dietary linseed oil and α-tocopherol on pork tenderloin (Psoas major) muscle. , 2003, Meat science.

[24]  J. Wood,et al.  Effect of a high-linolenic acid diet on lipogenic enzyme activities, fatty acid composition, and meat quality in the growing pig. , 2003, Journal of animal science.

[25]  J. Ruiz,et al.  EFFECT OF VITAMIN E SUPPLEMENTATION AND PARTIAL SUBSTITUTION OF POLY- WITH MONO-UNSATURATED FATTY ACIDS IN PIG DIETS ON MUSCLE, AND MICROSOME EXTRACT a-TOCOPHEROL CONCENTRATION AND LIPID OXIDATION , 2003, Archiv fur Tierernahrung.

[26]  H. J. Andersen,et al.  Manipulation of critical quality indicators and attributes in pork through vitamin E supplementation, muscle glycogen reducing finishing feeding and pre-slaughter stress. , 2002, Meat science.

[27]  I. Cambero,et al.  Effect of dietary linseed oil and α-tocopherol on selected properties of pig fat , 2002 .

[28]  R. Ofstad,et al.  Polyunsaturated fat and fish oil in diets for growing-finishing pigs: effects on fatty acid composition and meat, fat, and sausage quality. , 2002, Meat science.

[29]  U. Cogan,et al.  Influence of dietary vitamin E and selenium on muscle fatty acid composition in pigs , 2002 .

[30]  S. E. Harris,et al.  Antioxidant status affects color stability and tenderness of calcium chloride-injected beef. , 2001, Journal of animal science.

[31]  P. Calder,et al.  Effect of whole linseed (Linum usitatissimum) in the diet of finishing pigs on growth performance and on the quality and fatty acid composition of various tissues. , 2000, The British journal of nutrition.

[32]  J. Wiseman,et al.  Influence of type and dietary rate of inclusion of oil on meat quality of finishing pigs , 2000 .

[33]  P. Addis,et al.  Novel HPLC analysis of tocopherols, tocotrienols, and cholesterol in tissue. , 1999, Free radical biology & medicine.

[34]  D. Mottram.  Flavour formation in meat and meat products: a review , 1998 .

[35]  A. Schaefer,et al.  Distributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion-high-performance liquid chromatography , 1998, Lipids.

[36]  G. E. Onibi,et al.  Use of α-tocopherol acetate to improve fresh pig meat quality of full-fat rapeseed-fed pigs , 1998 .

[37]  A. Barroeta,et al.  Effect of Feeding Highly Cis-Monounsaturated, Trans, or n−3 Fats on Lipid Composition of Muscle and Adipose Tissue of Pigs , 1997 .

[38]  L. Skibsted,et al.  Oxidation in pre-cooked minced pork as influenced by chill storage of raw muscle. , 1997, Meat science.

[39]  D. Ahn,et al.  Effects of dietary α-linolenic acid on the fatty acid composition, storage stability and sensory characteristics of pork loin. , 1996, Meat science.

[40]  S. J. Jadhav,et al.  Lipid Oxidation in Biological and Food Systems , 1995 .

[41]  P. Morrissey,et al.  Influence of dietary vitamin E on the oxidative stability and quality of pig meat. , 1995, Journal of animal science.

[42]  D. Wulf,et al.  Effects of ground flaxseed in swine diets on pig performance and on physical and sensory characteristics and omega-3 fatty acid content of pork: I. Dietary level of flaxseed. , 1995, Journal of animal science.

[43]  D. Wulf,et al.  Effects of ground flaxseed in swine diets on pig performance and on physical and sensory characteristics and omega-3 fatty acid content of pork: II. Duration of 15% dietary flaxseed. , 1995, Journal of animal science.

[44]  M. C. Lanari,et al.  Dietary vitamin E supplementation and discoloration of pork bone and muscle following modified atmosphere packaging. , 1995, Meat science.

[45]  E. Olfert,et al.  Guide to the care and use of experimental animals , 1993 .

[46]  E. Kornegay,et al.  Humoral and cell-mediated immune response and performance of weaned pigs fed four supplemental vitamin E levels and housed at two nursery temperatures. , 1990, Journal of animal science.

[47]  P. B. Lynch,et al.  Effect of dietary α-tocopherol supplementation on α-tocopherol levels in porcine tissues and on susceptibility to lipid peroxidation , 1989 .

[48]  A. Booren,et al.  Effects of Dietary Oils and α-Tocopherol Supplementation on Lipid Composition and Stability of Broiler Meat , 1989 .

[49]  A. Booren,et al.  Effects of Dietary Antioxidants and Oxidized Oil on Membranal Lipid Stability and Pork Product Quality , 1989 .

[50]  L. Jönsson,et al.  Vitamin E requirement of growing swine. , 1988, Journal of animal science.

[51]  B. Halliwell.  Oxidants and human disease: some new concepts 1 , 1987, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[52]  R. Willson,et al.  Biological Antioxidants [and Discussion] , 1985 .

[53]  Board on Agriculture,et al.  Nutrient requirements of swine , 1964 .

[54]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[55]  The Use of Antioxidants in Foods , 1954 .