Vascular endothelial growth factor is expressed in rat corpus luteum.

In the course of the development of the ovarian follicle and differentiation of granulosa cells into corpus luteum (CL), extensive changes in the microvasculature of these structures take place. This suggests the local release of angiogenic factors. In the present work we examined whether a newly described secreted vascular endothelial growth factor (VEGF) is expressed in normal rat ovary by in situ hybridization. Our results demonstrate the expression of VEGF in the CL but not in mural granulosa cells, suggesting a temporal relation between VEGF expression and growth of capillary vessels. The hybridization pattern in the CL was consistent with localization of VEGF message to luteal cells. Expression of VEGF was detected also in cumulus oophorus cells. These findings suggest that VEGF is involved in the process of CL angiogenesis.

[1]  K. Gallagher,et al.  Physiological and immunocytochemical evidence for a new concept of blood flow regulation in the corpus luteum. , 1990, Biology of reproduction.

[2]  D. Goeddel,et al.  Vascular endothelial growth factor is a secreted angiogenic mitogen. , 1989, Science.

[3]  D. Connolly,et al.  Vascular permeability factor, an endothelial cell mitogen related to PDGF. , 1989, Science.

[4]  P. Seeburg,et al.  Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family. , 1989, Science.

[5]  N. Ferrara,et al.  Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. , 1989, Biochemical and biophysical research communications.

[6]  M. Kirschner,et al.  Synergistic induction of mesoderm by FGF and TGF-β and the identification of an mRNA coding for FGF in the early xenopus embryo , 1987, Cell.

[7]  R. Mitchell,et al.  Bovine granulosa cells produce basic fibroblast growth factor. , 1987, Endocrinology.

[8]  D. Gospodarowicz,et al.  Structural characterization and biological functions of fibroblast growth factor. , 1987, Endocrine reviews.

[9]  J. Slack,et al.  Mesoderm induction in early Xenopus embryos by heparin-binding growth factors , 1987, Nature.

[10]  H. Dvorak,et al.  A highly conserved vascular permeability factor secreted by a variety of human and rodent tumor cell lines. , 1986, Cancer research.

[11]  J. Fiddes,et al.  Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor. , 1986, Science.

[12]  R. Koos Stimulation of endothelial cell proliferation by rat granulosa cell-conditioned medium. , 1986, Endocrinology.

[13]  P. Bohlen,et al.  Corpus luteum angiogenic factor is related to fibroblast growth factor. , 1985, Endocrinology.

[14]  B. Zetter,et al.  The nonluteal porcine ovary as a source of angiogenic activity. , 1984, Endocrinology.

[15]  G. diZerega,et al.  Initiation of angiogenesis by human follicular fluid. , 1984, Science.

[16]  P. Oehme,et al.  Demonstration of angiogenesis-activity in the corpus luteum of cattle. , 1977, Experimentelle Pathologie.

[17]  L. Mastroianni,et al.  Capacitation, ovum maturation, fertilization and preimplantation development in the oviduct. , 1975, Gynecologic investigation.

[18]  E. Novak Gynecologic and obstetric pathology, with clinical and endocrine relations , 1947 .

[19]  D. L. Bassett The changes in the vascular pattern of the ovary of the albino rat during the estrous cycle , 1943 .