Characterization of Proteus mirabilis Lytic Phage from Situ Letik River, Bogor, Indonesia

Proteus mirabilis isolated from the feces of diarrhea patients was resistant to ampicillin, amoxicillin and trimethoprim-sulfomethaxazole. Therefore, to reduce potential infection of antibioticresistant P. mirabilis, alternative solutions such as application of lytic phage were needed. The aims of this study were to isolate and characterize phage that can lyse P. mirabilis cells. Phages were isolated from poultry waste and river water in Situ Letik village. Phage isolates were determined by their ability to form plaques, specificity, characteristics of protein and morphology. Phage was specific to P. mirabilis as its host. Sodium Dodecyl Sulphate-Poly Acrilamide Gel Electrophoresis (SDS-PAGE) analysis showed that the phage virion contains 8 proteins with molecular weight between 20-91 kDa. Transmission electron microscope observation showed that the phage was the member of Myoviridae family with icosahedral hexagonal head, the diameter about 68.185 nm, the length of tail about 109.019 nm, and the diameter of the tail about 17.89 nm width.

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