Optimization of hydrogen production from food waste using anaerobic mixed cultures pretreated with waste frying oil

Abstract The fermentation of food waste by anaerobic mixed cultures pretreated with waste frying oil (WFO) was examined in this study. Waste frying oil was used as a stressing agent to suppress hydrogenotrophic methanogens and enrich H2-producing bacteria. Lipid-rich compounds can be adsorbed onto the cell wall of some species, including methanogens, and they can reduce the permeability of the membranes and limit nutrient transport into cells. The optimization of H2 yield was performed using a three-factor, three-level Box-Behnken design method. Initial pH, pretreatment duration and waste frying oil concentration were considered the experimental factors. Pretreatment with waste frying oil decreased the production of CH4 significantly and, in turn, improved H2 accumulation. The response surface model predicted complete inhibition of methanogens with 7.74 g/L waste frying oil, an initial pH of 5.5 and a duration of 42.67 h for the pretreatment conditions. Applying these conditions led to an experimental H2 yield of 71.34 mL/gVS, which was significantly higher than that of untreated cultures (12.97 mL/gVS).

[1]  Zhong Hu,et al.  Statistical optimization of fermentative hydrogen production from xylose by newly isolated Enterobacter sp. CN1 , 2010 .

[2]  Subba Rao Chaganti,et al.  Optimizing hydrogen production from a switchgrass steam exploded liquor using a mixed anaerobic culture in an upflow anaerobic sludge blanket reactor , 2014 .

[3]  Y. Le Bihan,et al.  Long chain fatty acids (LCFA) evolution for inhibition forecasting during anaerobic treatment of lipid-rich wastes: Case of milk-fed veal slaughterhouse waste. , 2017, Waste management.

[4]  A. Stams,et al.  Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei , 2016, Microbial biotechnology.

[5]  J. Lalman,et al.  Impact of Initial pH and Linoleic Acid (C18:2) on Hydrogen Production by a Mesophilic Anaerobic Mixed Culture , 2008 .

[6]  R. Rafieenia,et al.  Pre-treating anaerobic mixed microflora with waste frying oil: A novel method to inhibit hydrogen consumption. , 2018, Waste management.

[7]  P. Ndegwa,et al.  Biohydrogen Production by Mesophilic Anaerobic Fermentation of Glucose in the Presence of Linoleic Acid , 2007 .

[8]  F. Arnold,et al.  Engineering microbial consortia: a new frontier in synthetic biology. , 2008, Trends in biotechnology.

[9]  A. Schievano,et al.  Dark fermentation metabolic models to study strategies for hydrogen consumers inhibition. , 2018, Bioresource technology.

[10]  D. Fino,et al.  Selection of the best pretreatment for hydrogen and bioethanol production from olive oil waste products , 2016 .

[11]  A Polettini,et al.  A review of dark fermentative hydrogen production from biodegradable municipal waste fractions. , 2013, Waste management.

[12]  A. Stams,et al.  Effect of long-chain fatty acids (LCFA) on the prevalence and viability of hydrogenotrophic methanogens , 2013 .

[13]  J. Lalman,et al.  Statistical optimization of factors affecting biohydrogen production from xylose fermentation using inhibited mixed anaerobic cultures , 2012 .

[14]  N. Ren,et al.  Microbial community structure of ethanol type fermentation in bio-hydrogen production. , 2007, Environmental microbiology.

[15]  A. Tsygankov,et al.  Measuring the pH dependence of hydrogenase activities , 2007, Biochemistry (Moscow).

[16]  F. Liu,et al.  Evaluation of different pretreatment methods for preparing hydrogen-producing seed inocula from waste activated sludge , 2011 .

[17]  J. Lalman,et al.  Effects of C18 long chain fatty acids on glucose, butyrate and hydrogen degradation. , 2002, Water research.

[18]  Haile Ma,et al.  Pretreatment of vinegar residue and anaerobic sludge for enhanced hydrogen and methane production in the two-stage anaerobic system , 2015 .

[19]  J. Lalman,et al.  Influence of linoleic acid, pH and HRT on anaerobic microbial populations and metabolic shifts in ASBRs during dark hydrogen fermentation of lignocellulosic sugars , 2013 .

[20]  Alfons J. M. Stams,et al.  Waste lipids to energy: how to optimize methane production from long‐chain fatty acids (LCFA) , 2009, Microbial biotechnology.

[21]  Jun Cheng,et al.  Investigating hydrothermal pretreatment of food waste for two-stage fermentative hydrogen and methane co-production. , 2017, Bioresource technology.

[22]  Luigi Frunzo,et al.  Dark fermentation of complex waste biomass for biohydrogen production by pretreated thermophilic anaerobic digestate. , 2015, Journal of environmental management.

[23]  Jun Hu,et al.  Enriching hydrogen-producing bacteria from digested sludge by different pretreatment methods , 2014 .

[24]  Alissara Reungsang,et al.  Co-digestion of food waste and sludge for hydrogen production by anaerobic mixed cultures: Statistic , 2011 .

[25]  J. Lalman,et al.  Assessing the impact of palmitic, myristic and lauric acids on hydrogen production from glucose fermentation by mixed anaerobic granular cultures , 2012 .

[26]  Dong-Hoon Kim,et al.  Flux balance analysis of mixed anaerobic microbial communities: Effects of linoleic acid (LA) and pH , 2011 .

[27]  Hisham Hafez,et al.  Ultrasonication for biohydrogen production from food waste , 2011 .

[28]  H. Hou,et al.  Statistical optimization of process parameters on biohydrogen production from glucose by Clostridium sp. Fanp2. , 2008, Bioresource technology.

[29]  J. Lalman,et al.  Pretreating mixed anaerobic communities from different sources: Correlating the hydrogen yield with hydrogenase activity and microbial diversity , 2012 .

[30]  L. Alibardi,et al.  Effects of carbohydrate, protein and lipid content of organic waste on hydrogen production and fermentation products. , 2016, Waste management.

[31]  R. Rafieenia,et al.  Effect of inoculum pre-treatment on mesophilic hydrogen and methane production from food waste using two-stage anaerobic digestion , 2018, International Journal of Hydrogen Energy.

[32]  J. Lalman,et al.  Long term impact of stressing agents on fermentative hydrogen production: Effect on the hydrogenase flux and population diversity , 2016 .

[33]  G. Izzo,et al.  Optimization of substrate composition for biohydrogen production from buffalo slurry co-fermented with cheese whey and crude glycerol, using microbial mixed culture , 2015 .

[34]  Zhaobo Chen,et al.  Effects of different pretreatment methods on fermentation types and dominant bacteria for hydrogen production , 2008 .

[35]  M. Taherzadeh,et al.  Inhibitory Effect of Long-Chain Fatty Acids on Biogas Production and the Protective Effect of Membrane Bioreactor , 2016, BioMed research international.

[36]  Mohd Ali Hassan,et al.  Food waste and food processing waste for biohydrogen production: a review. , 2013, Journal of environmental management.

[37]  G. Nakhla,et al.  The effect of heat pretreatment temperature on fermentative hydrogen production using mixed cultures , 2008 .

[38]  Poonsuk Prasertsan,et al.  Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis. , 2009, Bioresource technology.

[39]  D. Greenway,et al.  Mechanism of the inhibitory action of linoleic acid on the growth of Staphylococcus aureus. , 1979, Journal of general microbiology.

[40]  R. Rafieenia,et al.  Pre-treatment technologies for dark fermentative hydrogen production: Current advances and future directions. , 2018, Waste management.

[41]  J. Lalman,et al.  Effect of inhibitors on hydrogen consumption and microbial population dynamics in mixed anaerobic cultures , 2014 .

[42]  Alberto Pivato,et al.  Effect of aerobic pre-treatment on hydrogen and methane production in a two-stage anaerobic digestion process using food waste with different compositions. , 2017, Waste management.

[43]  A. Giordano,et al.  Evaluation of aeration pretreatment to prepare an inoculum for the two-stage hydrogen and methane production process. , 2014, Bioresource technology.

[44]  B. Mattiasson,et al.  Anaerobic digestion of lipid-rich waste - Effects of lipid concentration , 2007 .