AEBP 2 as a potential targeting protein for Polycomb Repression Complex PRC 2

AEBP2 is a zinc finger protein that has been shown to interact with the mammalian Polycomb Repression Complex 2 (PRC2). In the current study, we characterized this unknown protein and tested its potential targeting roles for the PRC2. AEBP2 is an evolutionarily well-conserved gene that is found in the animals ranging from flying insects to mammals. The transcription of mammalian AEBP2 is driven by two alternative promoters and produces at least two isoforms of the protein. These isoforms show developmental stage-specific expression patterns: the adult-specific larger form (51 kDa) and the embryo-specific smaller form (32 kDa). The AEBP2 protein binds to a DNA-binding motif with an unusual bipartite structure, CTT(N) 15-23cagGCC with lower-case being less critical. A large fraction of AEBP2’s target loci also map closely to the known target loci of the PRC2. In fact, many of these loci are co-occupied by the two proteins, AEBP2 and SUZ12. This suggests that AEBP2 is most likely a targeting protein for the mammalian

[1]  Y. Schwartz,et al.  Polycomb complexes and epigenetic states. , 2008, Current opinion in cell biology.

[2]  C. Villar,et al.  Programming of gene expression by Polycomb group proteins. , 2008, Trends in cell biology.

[3]  G. Schubiger,et al.  A screen for genes that function in leg disc regeneration in Drosophila melanogaster , 2008, Mechanisms of Development.

[4]  Jeong Do Kim,et al.  Retroposition and evolution of the DNA-binding motifs of YY1, YY2 and REX1 , 2007, Nucleic acids research.

[5]  Jürg Müller,et al.  Polycomb response elements and targeting of Polycomb group proteins in Drosophila. , 2006, Current opinion in genetics & development.

[6]  Hana Kim,et al.  An improved cloning strategy for chromatin-immunoprecipitation-derived DNA fragments. , 2006, Analytical Biochemistry.

[7]  J. Zeitlinger,et al.  Polycomb complexes repress developmental regulators in murine embryonic stem cells , 2006, Nature.

[8]  R. Mann,et al.  jing Is Required for Wing Development and to Establish the Proximo-Distal Axis of the Leg in Drosophila melanogaster , 2006, Genetics.

[9]  Malgorzata Schelder,et al.  A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities. , 2006, Genes & development.

[10]  B. Bonavida,et al.  Transcription factor YY1: structure, function, and therapeutic implications in cancer biology , 2006, Oncogene.

[11]  O. Cuvier,et al.  Recruitment of Drosophila Polycomb group proteins to chromatin by DSP1 , 2005, Nature.

[12]  Renato Paro,et al.  Epigenetic regulation of cellular memory by the Polycomb and Trithorax group proteins. , 2004, Annual review of genetics.

[13]  Yi Zhang,et al.  SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex. , 2004, Molecular cell.

[14]  Yi Zhang,et al.  The functions of E(Z)/EZH2-mediated methylation of lysine 27 in histone H3. , 2004, Current opinion in genetics & development.

[15]  Renato Paro,et al.  Genome-wide prediction of Polycomb/Trithorax response elements in Drosophila melanogaster. , 2003, Developmental cell.

[16]  Hengbin Wang,et al.  Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing , 2002, Science.

[17]  Y. Sedaghat,et al.  The jing Zn-finger transcription factor is a mediator of cellular differentiation in the Drosophila CNS midline and trachea. , 2002, Development.

[18]  D. Montell,et al.  Jing: a downstream target of slbo required for developmental control of border cell migration. , 2001, Development.

[19]  H. Ro,et al.  Cloning and Characterization of a Novel Zinc Finger Transcriptional Repressor , 1999, The Journal of Biological Chemistry.

[20]  M. Whiteley,et al.  The Drosophila Polycomb group gene pleiohomeotic encodes a DNA binding protein with homology to the transcription factor YY1. , 1998, Molecular cell.