About color combination using general VDT works, to clarify that feature color combination patterns considered usability are favorable color combination for human from the view point of fatigue, physiological data change during 30 min VDT works with low cognitive load were measured and subjective evaluation was carried out. In this experiment, as feature color combination patterns located on each quadrant in color combination image scale, black, blue, green and pink are used, and as fatigable color, cyan is used. As physiological data, ECG, pupil meter and GSR is measured. From the results of experiment, feature color combination patterns considered usability got higher evaluation from the view point of physiological data and subjective evaluation than a fatigable color combination. Therefore, we concluded that feature color combination patterns considered usability are no significant difference about fatigue and human can use these colors without fatigue.