Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method

Tubercle bacillus [TB] is one of the most important chronic infectious diseases that cause millions of deaths annually. While conventional smear microscopy and culture methods are widely used for diagnosis of TB, the former is insensitive, and the latter takes up to 6 to 8 weeks to provide a result, limiting the value of these methods in aiding diagnosis and intermediate decisions on treatment. Therefore, a rapid detection method is essential for the diagnosis, prognosis assessment, and recurrence monitoring. A new surface plasmon resonance [SPR] biosensor based on an array format, which allowed immobilizing nine TB antigens onto the sensor chip, was constructed. Simultaneous determination of multiple TB antibodies in serum had been accomplished with this array-based SPR system. The results were compared with enzyme-linked immunosorbent assay, a conventional immunological method. Array-based SPR showed more advantages in providing label-free and real-time detection. Additionally, the high sensitivity and specificity for the detection of TB infection showed its potential for future development of biosensor arrays for TB diagnosis.

[1]  L. Richeldi.  Rapid identification of Mycobacterium tuberculosis infection , 2006 .

[2]  K. Steingart,et al.  Commercial Serological Antibody Detection Tests for the Diagnosis of Pulmonary Tuberculosis: A Systematic Review , 2007, PLoS medicine.

[3]  A. Feinstein.  Clinical biostatistics; XXXIII. On teaching statistics to medical students , 1975, Clinical pharmacology and therapy.

[4]  Woon-Hong Yeo,et al.  Rapid detection of Mycobacterium tuberculosis cells by using microtip-based immunoassay , 2009, Analytical and bioanalytical chemistry.

[5]  E. Chan,et al.  Immunologic diagnosis of tuberculosis: a review. , 2000, Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.

[6]  Chia-Chen Chang,et al.  High-sensitivity detection of carbohydrate antigen 15-3 using a gold/zinc oxide thin film surface plasmon resonance-based biosensor. , 2010, Analytical chemistry.

[7]  R. Houghton,et al.  Assessment of the serodiagnostic potential of nine novel proteins from Mycobacterium tuberculosis. , 2001, FEMS microbiology letters.

[8]  S. Otsuki,et al.  Wavelength-scanning surface plasmon resonance imaging for label-free multiplexed protein microarray assay. , 2010, Biosensors & bioelectronics.

[9]  M. Perkins,et al.  Confronting the scientific obstacles to global control of tuberculosis. , 2008, The Journal of clinical investigation.

[10]  Surin Hong,et al.  Sensitive and molecular size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy , 2011, Nanoscale research letters.

[11]  Jacques Beauvais,et al.  Plasmonic Propagations Distances for Interferometric Surface Plasmon Resonance Biosensing , 2022 .

[12]  Sherry A. Dunbar,et al.  Identification and Differentiation of Clinically Relevant Mycobacterium Species Directly from Acid-Fast Bacillus-Positive Culture Broth , 2009, Journal of Clinical Microbiology.

[13]  D. Kernodle,et al.  Monitoring therapeutic efficacy by real-time detection of Mycobacterium tuberculosis mRNA in sputum. , 2009, Clinical chemistry.

[14]  Po-Ren Hsueh,et al.  A simple gold nanoparticle probes assay for identification of Mycobacterium tuberculosis and Mycobacterium tuberculosis complex from clinical specimens. , 2009, Molecular and cellular probes.

[15]  M. Perkins,et al.  Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. , 2006, The Lancet. Infectious diseases.

[16]  F. Drobniewski,et al.  For Personal Use. Only Reproduce with Permission from the Lancet Publishing Group. Modern Laboratory Diagnosis of Tuberculosis , 2022 .

[17]  S. Reed,et al.  Use of Multiepitope Polyproteins in Serodiagnosis of Active Tuberculosis , 2002, Clinical and Vaccine Immunology.

[18]  V. Adam,et al.  Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids , 2011, Nanoscale research letters.

[19]  Po-Ren Hsueh,et al.  Direct and simultaneous identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by rapid multiplex nested PCR-ICT assay. , 2006, Journal of microbiological methods.

[20]  C. Dye,et al.  Trends in tuberculosis incidence and their determinants in 134 countries. , 2009, Bulletin of the World Health Organization.

[21]  Chia-Chen Chang,et al.  An amplified surface plasmon resonance "turn-on" sensor for mercury ion using gold nanoparticles. , 2011, Biosensors & bioelectronics.

[22]  M. Gennaro.  Immunologic diagnosis of tuberculosis. , 2000, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[23]  Willem Haasnoot,et al.  Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins , 2011, Analytical and bioanalytical chemistry.

[24]  Günter Gauglitz,et al.  Direct optical detection in bioanalysis: an update , 2010, Analytical and bioanalytical chemistry.

[25]  Elvira Fortunato,et al.  Portable optoelectronic biosensing platform for identification of mycobacteria from the Mycobacterium tuberculosis complex. , 2011, Biosensors & bioelectronics.

[26]  B. Gicquel,et al.  Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation , 1991, The Lancet.

[27]  R. Corn,et al.  Fabrication of DNA microarrays with poly(L-glutamic acid) monolayers on gold substrates for SPR imaging measurements. , 2009, Langmuir : the ACS journal of surfaces and colloids.