Field Performance of Disease-Free Plants of Ginger Produced by Tissue Culture and Agronomic, Cytological, and Molecular Characterization of the Morphological Variants

Ginger (Zingiber officinale Rosc.) is an important spice crop valued for its flavored and medical properties. It is susceptible to soil-borne diseases, which can cause considerable economic loss to growers. In vitro culture is feasible for the propagation of disease-free ginger plants, but has several disadvantages when producing seed rhizomes that can be commercially used, such as long cultivation cycles (usually 2–3 years) and occurrence of somaclonal variation. In this study, dynamic changes in the morphological characteristics of in vitro-propagated disease-free plants of ‘Wuling’ ginger were evaluated by continuous observation and measurement at 30-day intervals, and morphological variants were screened and characterized by agronomic, cytological, and molecular analysis at harvest. Results showed that the plants grew rapidly within 120 days after planting, and the most active growth period was from 60 to 120 days. Eight plants with clear and stable morphological differences were screened out from approximately 2000 plants grown in the field, and they could be classified into two groups (VT1 and VT2) based on tiller number, plant height, leaf color, and leaf shape. By flow cytometry analysis and chromosome counting, the VT1 was confirmed to be diploid, with the shortest plant height, the largest number of tillers and rhizome knobs, and the smallest tiller diameter and rhizome size among the three types of plants. The VT2 was mixoploid, consisting of diploid and tetraploid cells, with significantly reduced tiller number and rhizome knobs, significantly larger stomatal guard cells/apertures, and significantly lower stomatal density. SSR analysis detected DNA band profile changes in six out of the eight variants, including one plant of the VT1 and all the VT2 plants. The findings of this study might contribute to the commercial production of disease-free seed rhizomes in ginger, and the characterized somaclonal variants could provide useful germplasm resources for future breeding.

[1]  Liu Hu,et al.  Morphological, Cytological, and Molecular-Based Genetic Stability Analysis of In Vitro-Propagated Plants from Newly Induced Aneuploids in Caladium , 2022, Agriculture.

[2]  X. Liu,et al.  Evaluation of the Contact Toxicity and Physiological Mechanisms of Ginger (Zingiber officinale) Shoot Extract and Selected Major Constituent Compounds against Melanaphis sorghi Theobald , 2022, Horticulturae.

[3]  K. Ioannidis,et al.  Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers , 2022, Plants.

[4]  V. Cristofori,et al.  In Vitro Polyploid Induction of Highbush Blueberry through De Novo Shoot Organogenesis , 2022, Plants.

[5]  D. Prasath,et al.  Effect of colchicine induced tetraploids of ginger (Zingiber officinale Roscoe) on cytology, rhizome morphology, and essential oil content , 2022, Journal of Applied Research on Medicinal and Aromatic Plants.

[6]  R. Kumar,et al.  A Comparative review on ginger and garlic with their pharmacological Action , 2022, Asian Journal of Pharmaceutical Research and Development.

[7]  N. Teixidó,et al.  Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions , 2022, Planta.

[8]  Jie Zhou,et al.  Efficient ex-vitro rooting and acclimatization for tissue culture plantlets of ginger , 2022, Plant Cell, Tissue and Organ Culture (PCTOC).

[9]  Jie Zhou,et al.  Silicon Nanoparticles Enhance Ginger Rhizomes Tolerance to Postharvest Deterioration and Resistance to Fusarium solani , 2022, Frontiers in Plant Science.

[10]  Farhan Nabi,et al.  Fungicidal properties of ginger (Zingiber officinale) essential oils against Phytophthora colocasiae , 2022, Scientific Reports.

[11]  Canbin Ouyang,et al.  Efficacy and economics evaluation of seed rhizome treatment combined with preplant soil fumigation on ginger soilborne disease, plant growth and yield promotion. , 2021, Journal of the science of food and agriculture.

[12]  Q. Xia,et al.  Haplotype-resolved genome of diploid ginger (Zingiber officinale) and its unique gingerol biosynthetic pathway , 2021, Horticulture Research.

[13]  H. Jaafar,et al.  Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application , 2021, Agronomy.

[14]  A. Trzewik,et al.  Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.) , 2020, Agronomy.

[15]  Chunjie Wu,et al.  Ginger (Zingiber officinale Rosc.) and its bioactive components are potential resources for health beneficial agents , 2020, Phytotherapy research : PTR.

[16]  M. Thakur,et al.  In vitro selection of gamma irradiated shoots of ginger (Zingiber officinale Rosc.) against Fusarium oxysporum f.sp. zingiberi and molecular analysis of the resistant plants , 2020, Plant Cell, Tissue and Organ Culture (PCTOC).

[17]  R. Kiyama Nutritional implications of ginger: Chemistry, biological activities and signaling pathways. , 2020, The Journal of nutritional biochemistry.

[18]  Xiao-Dong Cai,et al.  Morphological, cytological, and pigment analysis of leaf color variants regenerated from long-term subcultured caladium callus , 2020, In Vitro Cellular & Developmental Biology - Plant.

[19]  R. Pant,et al.  Association of two novel viruses with chlorotic fleck disease of ginger , 2020, Annals of Applied Biology.

[20]  W. Helmy,et al.  Identification of DNA variation in callus derived from Zingiber officinale and anticoagulation activities of ginger rhizome and callus , 2020, Bulletin of the National Research Centre.

[21]  Kehu Yang,et al.  Ginger for health care: An overview of systematic reviews. , 2019, Complementary therapies in medicine.

[22]  G. Thiagu,et al.  Indirect somatic embryogenesis and Agrobacterium-mediated transient transformation of ginger (Zingiber officinale Rosc.) using leaf sheath explants , 2019, The Journal of Horticultural Science and Biotechnology.

[23]  H. Corke,et al.  Bioactive Compounds and Bioactivities of Ginger (Zingiber officinale Roscoe) , 2019, Foods.

[24]  Merga Jibat Guji,et al.  Yield loss of ginger (Zingiber officinale) due to bacterial wilt (Ralstonia solanacearum) in different wilt management systems in Ethiopia , 2019, Agriculture & Food Security.

[25]  Xue Gao,et al.  Effects of ploidy level on the cellular, photochemical and photosynthetic characteristics in Lilium FO hybrids. , 2018, Plant physiology and biochemistry : PPB.

[26]  J. B. Pinheiro,et al.  Agronomic evaluation and clonal selection of ginger genotypes (Zingiber officinale Roscoe) in Brazil , 2017 .

[27]  J. Kennedy,et al.  Chitosan and oligochitosan enhance ginger (Zingiber officinale Roscoe) resistance to rhizome rot caused by Fusarium oxysporum in storage. , 2016, Carbohydrate polymers.

[28]  H. Krishna,et al.  Somaclonal variations and their applications in horticultural crops improvement , 2016, 3 Biotech.

[29]  Kun Xu,et al.  Natural occurrence of mixploid ginger (Zingiber officinale Rosc.) in China and its morphological variations , 2014 .

[30]  B. S. Bhau,et al.  Microsporogenesis and pollen formation in Zingiber officinale Roscoe , 2014, Plant Systematics and Evolution.

[31]  Gokare A. Ravishankar,et al.  Rapid in vitro regeneration method for Moringa oleifera and performance evaluation of field grown nutritionally enriched tissue cultured plants , 2012, 3 Biotech.

[32]  Rakesh Sharma,et al.  In vitro selection of resistant mutants of ginger (Zingiber officinale Rosc.) against wilt pathogen (Fusarium oxysporum f.sp. zingiberi Trujillo) , 2012 .

[33]  K. Jayarajan,et al.  Relationship between vegetative and rhizome characters and final rhizome yield in micropropagated ginger plants (Zingiber officinale Rosc.) over two generations , 2008 .

[34]  S. Tanksley,et al.  Microprep protocol for extraction of DNA from tomato and other herbaceous plants , 1995, Plant Molecular Biology Reporter.

[35]  Arnab Sen,et al.  Rapid in vitro multiplication of disease-free Zingiber officinale rosc , 2006 .

[36]  S. Kaeppler,et al.  Epigenetic aspects of somaclonal variation in plants , 2000, Plant Molecular Biology.

[37]  T. Sharma,et al.  High-frequency in vitro multiplication of disease-free Zingiber officinale Rosc. , 1997, Plant Cell Reports.

[38]  S. Hamill,et al.  Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland , 1996 .