Dicomization of LSM fluorescence composite microscopic image with its bioimaging information

In recent years, the quality of fluorescence microscopic imaging produced by a laser scanning microscope (LSM) system has been increased through the use of optical imaging techniques being used for small biological or nonbiological particles like instance, bacteria or cells in tissue samples. Currently, multiple manufactures provide LSM equipment able to capture single-layer or stacked images. However, the manufactures still using distinct data formats including proprietary ones, limiting the interoperability with third systems. In the clinical environment, the scanners need to be integrated with a vendor-neutral archive, storing images in a standard format and interface, making them accessible to healthcare professionals regardless of what proprietary system created it. Having distinct software solutions to manage the data is tiresome and time-consuming. This article proposes a normalization pipeline that can convert distinct vendor formats in a standard DICOM structure including pixel data and metadata. After conversion, the images are sent to a DICOM-compliant repository being able to be consumed in the network using normalized communication and visualization processes. The proposed solution is reliable and uses efficiently the less memory, a critical issue since the resolution of pathology whole-slide images can reach several gigapixels.