Micro-computed tomography for small animal imaging： Technological details

Abstract The high-resolution micro-computed tomography (micro-CT) system has now become an important tool for biological research. The micro-CT system enables a non-invasive inspection to screen anatomical changes in small animals. The promising advantages include high-spatial resolution, high sensitivity to bone and lung, short scan time and cost-effectiveness. The dose received by the small animal might be a critical concern in the research. In this article, the choice of the components, fundamental physical problems, the image reconstruction algorithm and the representative applications of micro-CT are summarized. Some results from our research group are also presented to show high-resolution images obtained by the micro-CT system.

[1]  M J Paulus,et al.  High resolution X-ray computed tomography: an emerging tool for small animal cancer research. , 2000, Neoplasia.

[2]  Dianna D Cody,et al.  In vivo respiratory-gated micro-CT imaging in small-animal oncology models. , 2004, Molecular imaging.

[3]  Maria Drangova,et al.  Prospective respiratory-gated micro-CT of free breathing rodents. , 2005, Medical physics.

[4]  S. Goldstein,et al.  Evaluation of a microcomputed tomography system to study trabecular bone structure , 1990, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[5]  B. F. Logan,et al.  The Fourier reconstruction of a head section , 1974 .

[6]  David Brasse,et al.  Towards an inline reconstruction architecture for micro-CT systems , 2005, Physics in medicine and biology.

[7]  Jens Gregor,et al.  Fast Feldkamp reconstruction based on focus of attention and distributed computing , 2002, Int. J. Imaging Syst. Technol..

[8]  Shaun S. Gleason,et al.  A new X-ray computed tomography system for laboratory mouse imaging , 1998 .

[9]  Eric C. Frey,et al.  Development and evaluation of a MicroCT system for small animal imaging , 2001, 2001 IEEE Nuclear Science Symposium Conference Record (Cat. No.01CH37310).

[10]  L. Hedlund,et al.  Micro-CT with respiratory and cardiac gating. , 2004, Medical physics.

[11]  K. Smith,et al.  SPECT-CT system for small animal imaging , 2001 .

[12]  F. Foster,et al.  Ultrasonic and viscoelastic properties of skin under transverse mechanical stress in vitro. , 1998, Ultrasound in medicine & biology.

[13]  H Miura,et al.  Effect of Vitamin K2 on Three‐Dimensional Trabecular Microarchitecture in Ovariectomized Rats , 2000, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[14]  F. H. Séguin,et al.  The largest and smallest X-ray computed tomography systems , 1984 .

[15]  S. Webb,et al.  Cone-beam x-ray microtomography of small specimens. , 1994, Physics in medicine and biology.

[16]  Gyuseong Cho,et al.  Performance evaluation of a flat-panel detector-based microtomography system for small-animal imaging , 2003, 2003 IEEE Nuclear Science Symposium. Conference Record (IEEE Cat. No.03CH37515).

[17]  R E Guldberg,et al.  Analyzing bone, blood vessels, and biomaterials with microcomputed tomography. , 2003, IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society.

[18]  Gabor T. Herman,et al.  Image reconstruction from projections : the fundamentals of computerized tomography , 1980 .

[19]  Simon R Cherry,et al.  Simultaneous molecular and anatomical imaging of the mouse in vivo , 2002, Physics in medicine and biology.

[20]  H H Barrett,et al.  High-resolution computed tomography of the normal rat nephrogram. , 1980, Investigative radiology.

[21]  J. Hecksher-Sørensen,et al.  Optical Projection Tomography as a Tool for 3D Microscopy and Gene Expression Studies , 2002, Science.

[22]  Henrik Turbell,et al.  Cone-Beam Reconstruction Using Filtered Backprojection , 2001 .

[23]  J A Rowlands,et al.  X-ray imaging using amorphous selenium: feasibility of a flat panel self-scanned detector for digital radiology. , 1995, Medical physics.

[24]  P. Callaghan,et al.  High-resolution imaging. The NMR microscope , 1986 .

[25]  L. Grodzins,et al.  Optimum energies for x-ray transmission tomography of small samples. Applications of synchrotron radiation to computerized tomography I , 1983 .

[26]  T Sato,et al.  X-ray tomography for microstructural objects. , 1981, Applied optics.

[27]  L. Feldkamp,et al.  Practical cone-beam algorithm , 1984 .

[28]  Maria Drangova,et al.  Prospective respiratory-gated micro-CT of free breathing rodents. , 2005, Medical physics.

[29]  Zhi-pei Liang,et al.  Introduction to biomedical imaging , 2008 .

[30]  John M. Ollinger,et al.  Maximum-likelihood reconstruction of transmission images in emission computed tomography via the EM algorithm , 1994, IEEE Trans. Medical Imaging.

[31]  S M Jorgensen,et al.  Three-dimensional imaging of vasculature and parenchyma in intact rodent organs with X-ray micro-CT. , 1998, The American journal of physiology.

[32]  M. Glas,et al.  Principles of Computerized Tomographic Imaging , 2000 .

[33]  P Rüegsegger,et al.  Micro-CT examinations of trabecular bone samples at different resolutions: 14, 7 and 2 micron level. , 1998, Technology and health care : official journal of the European Society for Engineering and Medicine.

[34]  J H Siewerdsen,et al.  Cone-beam computed tomography with a flat-panel imager: initial performance characterization. , 2000, Medical physics.

[35]  H. Atkins,et al.  Pinhole SPECT: an approach to in vivo high resolution SPECT imaging in small laboratory animals. , 1994, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[36]  Natalia Auricchio,et al.  CT with a CMOS flat panel detector integrated on the YAP-(S)PET scanner for in vivo small animal imaging , 2007 .

[37]  S. Goldstein,et al.  The direct examination of three‐dimensional bone architecture in vitro by computed tomography , 1989, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[38]  Anita H. Dhyani,et al.  Microfocal X-ray CT imaging and pulmonary arterial distensibility in excised rat lungs. , 2001, American journal of physiology. Heart and circulatory physiology.

[39]  H. B. Barber,et al.  Compact CT/SPECT small-animal imaging system , 2002, IEEE Transactions on Nuclear Science.

[40]  K. Lange,et al.  EM reconstruction algorithms for emission and transmission tomography. , 1984, Journal of computer assisted tomography.

[41]  G. Allan Johnson,et al.  Nuclear magnetic resonance imaging at microscopic resolution , 1986 .

[42]  Alexander G Robling,et al.  Improved Bone Structure and Strength After Long‐Term Mechanical Loading Is Greatest if Loading Is Separated Into Short Bouts , 2002, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[43]  E A Hoffman,et al.  In vivo micro-CT lung imaging via a computer-controlled intermittent iso-pressure breath hold (IIBH) technique , 2006, Physics in medicine and biology.

[44]  Ralph Müller,et al.  Mechanical and Architectural Bone Adaptation in Early Stage Experimental Osteoarthritis , 2002, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[45]  J A Rowlands,et al.  X-ray detectors for digital radiography. , 1997, Physics in medicine and biology.

[46]  Erik L Ritman,et al.  The use of microcomputed tomography to study microvasculature in small rodents. , 2002, American journal of physiology. Regulatory, integrative and comparative physiology.

[47]  R E Jacobs,et al.  Looking deeper into vertebrate development. , 1999, Trends in cell biology.

[48]  James F. Young,et al.  MicroPET: a high resolution PET scanner for imaging small animals , 1996, IEEE Nuclear Science Symposium Conference Record.

[49]  J M Boone,et al.  Determination of the presampled MTF in computed tomography. , 2001, Medical physics.

[50]  Min Hyoung Cho,et al.  A flat-panel detector based micro-CT system: performance evaluation for small-animal imaging. , 2003, Physics in medicine and biology.

[51]  James A Bankson,et al.  Improved method of in vivo respiratory-gated micro-CT imaging , 2004, Physics in medicine and biology.

[52]  Françoise Peyrin,et al.  Parallel Image Reconstruction on MIMD Computers for Three-Dimensional Cone-Beam Tomography , 1998, Parallel Comput..

[53]  Otto Zhou,et al.  Carbon nanotube based microfocus field emission x-ray source for microcomputed tomography , 2006 .

[54]  Eric L. Miller,et al.  Parallel-Beam Backprojection: An FPGA Implementation Optimized for Medical Imaging , 2002, FPGA '02.

[55]  J. Kinney,et al.  Relationship Between Plain Radiographic Patterns and Three- dimensional Trabecular Architecture in The Human Calcaneus , 1999, Osteoporosis International.

[56]  Ishwar K. Sethi,et al.  Cone beam tomography using MPI on heterogeneous workstation clusters , 1996, Proceedings. Second MPI Developer's Conference.

[57]  R Müller,et al.  Genetic Regulation of Cortical and Trabecular Bone Strength and Microstructure in Inbred Strains of Mice , 2000, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[58]  Joaquim Salvi,et al.  Review of CMOS image sensors , 2006, Microelectron. J..

[59]  B Nebiyou Bekele,et al.  Murine Lung Tumor Measurement Using Respiratory-Gated Micro-Computed Tomography , 2005, Investigative radiology.

[60]  E. Niki,et al.  Prevention of Type I Diabetes by Low-Dose Gamma Irradiation in NOD Mice , 2000, Radiation research.

[61]  B. De Man,et al.  Distance-driven projection and backprojection in three dimensions. , 2004, Physics in medicine and biology.

[62]  Gang Hu,et al.  Micro-CT in a dual-modality fluorescence/computed tomography system for small animal imaging , 2007, 2007 IEEE Nuclear Science Symposium Conference Record.

[63]  Horst Halling,et al.  Preliminary studies of a micro-CT for a combined small animal PET/CT scanner , 2001, 2001 IEEE Nuclear Science Symposium Conference Record (Cat. No.01CH37310).

[64]  Peter J. Gregory,et al.  An X-ray micro-tomography system optimised for the low-dose study of living organisms. , 2003, Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine.

[65]  Ge Wang,et al.  Fast Algorithm for X-ray Cone-beam Microtomography. , 2001, Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada.

[66]  Fang Xu,et al.  Accelerating popular tomographic reconstruction algorithms on commodity PC graphics hardware , 2005, IEEE Transactions on Nuclear Science.

[67]  J. E. Bigelow,et al.  The Demands On The a-Si FET As A Pixel Switch For Liquid Crystal Displays , 1986, Photonics West - Lasers and Applications in Science and Engineering.

[68]  Ho Kyung Kim,et al.  Use of a flat-panel detector for microtomography: a feasibility study for small-animal imaging , 2005, IEEE Transactions on Nuclear Science.

[69]  D Van Dyck,et al.  In vivo assessment of emphysema in mice by high resolution X‐ray microtomography , 2005, Journal of microscopy.

[70]  Kunio Doi,et al.  A simple method for determining the modulation transfer function in digital radiography , 1992, IEEE Trans. Medical Imaging.

[71]  Yuan Cheng,et al.  Development of a carbon nanotube based microfocus x-ray tube with single focusing electrode , 2006 .

[72]  Sharmila Majumdar,et al.  MicroCT evaluation of normal and osteoarthritic bone structure in human knee specimens , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[73]  Aaron Fenster,et al.  Tabletop CT system for high-resolution volume imaging , 1990, Medical Imaging.

[74]  M. J. Powell.  The physics of amorphous-silicon thin-film transistors , 1989 .

[75]  G. Baldazzi,et al.  A High Spatial Resolution CT Scanner for Small Animal Imaging , 2006 .

[76]  Michael J. Flynn,et al.  Microfocus X-ray sources for 3D microtomography , 1994 .

[77]  G. E. Smith,et al.  Charge coupled semiconductor devices , 1970, Bell Syst. Tech. J..

[78]  H. Tuy.  AN INVERSION FORMULA FOR CONE-BEAM RECONSTRUCTION* , 1983 .

[79]  R. Street,et al.  A comparison of x-ray detectors for mouse CT imaging , 2004, Physics in medicine and biology.

[80]  Dirk Van Dyck,et al.  High-resolution X-ray microtomography for the detection of lung tumors in living mice. , 2004, Neoplasia.

[81]  E L Ritman,et al.  Three-dimensional microcomputed tomography of renal vasculature in rats. , 1998, Hypertension.

[82]  Yuan Cheng,et al.  A nanotube-based field emission x-ray source for microcomputed tomography , 2005 .

[83]  Kenneth H. Wong,et al.  Dual-modality imaging of function and physiology. , 2002, Academic radiology.