Recognition of Rhodobacter sphaeroides by microcontact-imprinted poly(ethylene-co-vinyl alcohol).

The immobilization of cells or microorganisms is important for bioseparations, in bioreactors producing cellular metabolites, and as receptors for biosensing. Cell-imprinted polymers (CIPs) have been shown to have cavities with complementary shapes and also high affinities for the template cells or microorganisms. However, the effects of binding to CIPs on gene expression are only beginning to be studied. In this work, the purple bacteria Rhodobacter sphaeroides was employed as a model for the imprinting of microorganisms. R. sphaeroides was first adsorbed on a glass slide as the stamp and then microcontact-imprinted onto poly(ethylene-co-vinyl alcohol), EVAL. The surfaces of the R. sphaeroides-imprinted (RsIPs) and non-imprinted (NIPs) EVAL thin films were examined by Raman spectrometry and scanning electron microscopy. The expression of the nitrogenase (nitrogen fixation, nifH) gene of R. sphaeroides adsorbed on both the RsIPs and NIPs EVAL thin films was also measured by the quantitative reverse transcription polymerase chain reaction (qRT-PCR); cells grown on imprinted polymer showed dramatic differences in gene expression compared to controls.

[1]  Yuriy Rebets,et al.  SimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors , 2012, AMB Express.

[2]  J. Hubbell,et al.  Force Measurements between Bacteria and Poly(ethylene glycol)-Coated Surfaces , 2000 .

[3]  F. Dickert,et al.  Bioimprinting of polymers and sol-gel phases. Selective detection of yeasts with imprinted polymers. , 2002, Analytical chemistry.

[4]  F. Dickert,et al.  Synthetic receptors as sensor coatings for molecules and living cells. , 2001, The Analyst.

[5]  H. P. Bennetto,et al.  Microbial fuel-cells , 1993 .

[6]  Peter A. Lieberzeit,et al.  Artificial Antibodies for Bioanalyte Detection—Sensing Viruses and Proteins , 2006 .

[7]  H. C. van der Mei,et al.  Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces. , 2008, Microbiology.

[8]  B. Logan,et al.  Immobilization of anode-attached microbes in a microbial fuel cell , 2012, AMB Express.

[9]  I. Eroglu,et al.  Effects of ammonium ion, acetate and aerobic conditions on hydrogen production and expression levels of nitrogenase genes in Rhodobacter sphaeroides O.U.001 , 2009 .

[10]  Mei-Hwa Lee,et al.  Microcontact imprinting of algae on poly(ethylene-co-vinyl alcohol) for biofuel cells. , 2013, ACS applied materials & interfaces.

[11]  Shunsaku Ueda,et al.  Hydrogen production as a novel process of wastewater treatment—studies on tofu wastewater with entrapped R. sphaeroides and mutagenesis , 2002 .

[12]  James L. Thomas,et al.  Sensing of digestive proteins in saliva with a molecularly imprinted poly(ethylene-co-vinyl alcohol) thin film coated quartz crystal microbalance sensor. , 2011, ACS applied materials & interfaces.

[13]  I. Eroglu,et al.  Photofermentative Hydrogen Production in Outdoor Conditions , 2012 .

[14]  Daniel H. Buckley,et al.  A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase , 2012, PloS one.

[15]  I. Karube,et al.  Biochemical fuel cell utilizing immobilized cells of clostridium butyricum , 1977 .

[16]  H. C. van der Mei,et al.  Statistical Analysis of Long- and Short-Range Forces Involved in Bacterial Adhesion to Substratum Surfaces as Measured Using Atomic Force Microscopy , 2011, Applied and Environmental Microbiology.

[17]  M. Tonetti,et al.  Roughness response genes in osteoblasts. , 2004, Bone.

[18]  Yong Chen,et al.  Cell imprinting and AFM imaging of cells cultured on nanoline patterns , 2010 .

[19]  P. Atanassov,et al.  New materials for biological fuel cells , 2012 .

[20]  M. Mahmoudi,et al.  Cell-imprinted substrates direct the fate of stem cells. , 2013, ACS nano.

[21]  Tingyue Gu,et al.  A state of the art review on microbial fuel cells: A promising technology for wastewater treatment and bioenergy. , 2007, Biotechnology advances.

[22]  Mukul M. Sharma,et al.  Adhesion Forces between E. c oli Bacteria and Biomaterial Surfaces , 1999 .

[23]  W. Verstraete,et al.  Microbial fuel cells: novel biotechnology for energy generation. , 2005, Trends in biotechnology.

[24]  Seokheun Choi,et al.  Technology and Applications of Microbial Biosensor , 2013 .

[25]  Franz L Dickert,et al.  Sensing picornaviruses using molecular imprinting techniques on a quartz crystal microbalance. , 2009, Analytical chemistry.

[26]  L. Seefeldt,et al.  Nitrogenase: a draft mechanism. , 2013, Accounts of chemical research.

[27]  A. K. Shukla,et al.  Biological fuel cells and their applications , 2004 .

[28]  M. Daemen,et al.  Selective identification of macrophages and cancer cells based on thermal transport through surface-imprinted polymer layers. , 2013, ACS applied materials & interfaces.

[29]  Eric W. Hall,et al.  Separation of bacteria with imprinted polymeric films. , 2012, In Analysis.

[30]  D. Pant,et al.  A review of the substrates used in microbial fuel cells (MFCs) for sustainable energy production. , 2010, Bioresource technology.

[31]  Eileen M. Spain,et al.  Characterizing Pilus-Mediated Adhesion of Biofilm-Forming E. coli to Chemically Diverse Surfaces Using Atomic Force Microscopy , 2013, Langmuir : the ACS journal of surfaces and colloids.

[32]  Bruce E. Logan,et al.  Microbial Fuel Cells , 2006 .

[33]  M. Pfaffl,et al.  A new mathematical model for relative quantification in real-time RT-PCR. , 2001, Nucleic acids research.

[34]  Ashley E. Franks,et al.  Microbial Fuel Cells, A Current Review , 2010 .

[35]  R. Zare,et al.  Sorting inactivated cells using cell-imprinted polymer thin films. , 2013, ACS nano.

[36]  A. Tsygankov.  Hydrogen photoproduction by purple bacteria: Immobilized vs. Suspension cultures , 2001 .

[37]  Geunhyung Kim,et al.  Effects of a cell-imprinted poly(dimethylsiloxane) surface on the cellular activities of MG63 osteoblast-like cells: preparation of a patterned surface, surface characterization, and bone mineralization. , 2012, Langmuir : the ACS journal of surfaces and colloids.