Three-Dimensional Cell Growth on Structures Fabricated from ORMOCER® by Two-Photon Polymerization Technique

Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic—inorganic hybrid polymer ORMOCER®. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER®. ORMOCER® related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER® is shown.

[1]  A. Voigt,et al.  Adhesion of leucocytes to microscope slides as influenced by electrostatic interaction. , 1995, Biomaterials.

[2]  Alexander Pilger,et al.  Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts. , 2002, Mutation research.

[3]  P. Dannberg,et al.  ORMOCER®s for Optical Interconnection Technology , 2001 .

[4]  A. Ngezahayo,et al.  Regulation of Ion Fluxes, Cell Volume and Gap Junctional Coupling by cGMP in GFSHR-17 Granulosa Cells , 2003, The Journal of Membrane Biology.

[5]  Herbert Wolter,et al.  Synthesis, properties and applications of inorganic–organic copolymers (ORMOCER®s) , 1999 .

[6]  B N Chichkov,et al.  Femtosecond laser-induced two-photon polymerization of inorganic-organic hybrid materials for applications in photonics. , 2003, Optics letters.

[7]  P. Allen,et al.  Mechanical culture conditions effect gene expression: gravity-induced changes on the space shuttle. , 2000, Physiological genomics.

[8]  A. Ngezahayo,et al.  Gap Junction Coupling and Apoptosis in GFSHR-17 Granulosa Cells , 2005, The Journal of Membrane Biology.

[9]  C. Gimkiewicz,et al.  Replicated optical MEMS in sol-gel materials , 2004, IEEE Journal of Selected Topics in Quantum Electronics.

[10]  C. Fotakis,et al.  Fabrication of three-dimensional structures by three-photon polymerization. , 2005, Optics letters.

[11]  K. Fujimoto,et al.  Dynamics of connexins, E-cadherin and alpha-catenin on cell membranes during gap junction formation. , 1997, Journal of cell science.

[12]  Dietmar W. Hutmacher,et al.  Scaffold design and fabrication technologies for engineering tissues — state of the art and future perspectives , 2001, Journal of biomaterials science. Polymer edition.

[13]  Kenneth M. Yamada,et al.  Taking Cell-Matrix Adhesions to the Third Dimension , 2001, Science.

[14]  B. Chichkov,et al.  Two photon induced polymerization of organic-inorganic hybrid biomaterials for microstructured medical devices. , 2006, Acta biomaterialia.

[15]  M. Sefton,et al.  Tissue engineering. , 1998, Journal of cutaneous medicine and surgery.

[16]  Aleksandr Ovsianikov,et al.  Fabrication of woodpile structures by two-photon polymerization and investigation of their optical properties. , 2004, Optics express.