Microsatellite Markers Associated with Spot Blotch Resistance in Spring Wheat

Spot blotch, caused by Cochliobolus sativus, is a serious wheat (Triticum aestivum L.) disease in the warm areas of South Asia. Breeding for resistance in the past 15 years has produced limited progress, and newly developed wheat cultivars suffer considerable yield reductions under spot blotch epidemics in the region. Resistance is often controlled by multiple genes with additive effects. Marker-assisted selection, in combination with field selection, could accelerate the identification of progeny with multiple genes for resistance early in the breeding process. A study was conducted to determine microsatellite markers associated with resistance in the F 7 progeny from a cross between the spot blotch-susceptible Sonalika and resistant G162 wheat genotypes. A parental survey using 171 simple sequence repeats (SSR) primer sets and spread over 21 chromosomes of wheat identified 52% polymorphic loci. However, only 15 polymorphic markers showed association with two bulks, one each of progeny with low and with high spot blotch severity. The detailed analysis indicated that progeny lines with low spot blotch severity could be separated from those with high severity using three SSR markers located on three wheat chromosomes. The findings may be useful in developing a marker-assisted selection strategy for spot blotch resistance in wheat.

[1]  E. Duveiller,et al.  Spot Blotch Continues to Cause Substantial Grain Yield Reductions under Resource-limited Farming Conditions , 2006 .

[2]  A. Siddique,et al.  Progress in wheat resistance to spot blotch in Bangladesh , 2006 .

[3]  E. Duveiller,et al.  Epidemiology of foliar blights (spot blotch and tan spot) of wheat in the plains bordering the himalayas. , 2005, Phytopathology.

[4]  E. Duveiller,et al.  Combining ability analysis of resistance to Helminthosporium leaf blight in spring wheat , 2004, Euphytica.

[5]  Ashutosh Kumar Singh,et al.  Helminthosporium leaf blight resistance and agronomic performance of wheat genotypes across warm regions of South Asia , 2004 .

[6]  K. Prabhu,et al.  Recessive genes controlling resistance to Helminthosporium leaf blight in synthetic hexaploid wheat , 2004 .

[7]  K. Edwards,et al.  A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.) , 2004, Theoretical and Applied Genetics.

[8]  R. Chand,et al.  Inheritance of resistance to spot blotch caused by bipolaris sorokiniana in spring wheat , 2004 .

[9]  E. Duveiller.  Controlling Foliar Blights of Wheat in the Rice-Wheat Systems of Asia. , 2004, Plant disease.

[10]  E. Duveiller,et al.  Resistance to Helminthosporium leaf blight and agronomic performance of spring wheat genotypes of diverse origins , 2004, Euphytica.

[11]  M. Ganal,et al.  A microsatellite map of wheat. , 1998, Genetics.

[12]  C. Mundt,et al.  Inheritance of Slow‐Rusting Resistance to Leaf Rust in Wheat , 1992 .