Combined effects of plant growth promoting rhizobacteria and fungi on mung bean (Vigna radiata L.)

In the present study, screened PGPR and Fungi were influence the growth of Mung bean (Vigna radiata) plant in the pot. Two rhizobacteria viz. Rhizobium sp., Pseudomonas putida and three fungi Aspergillus niger, Rhizopus sp. and Trichoderma viride were isolated and purified. The effect of inoculation of different strains of bacteria and fungus on growth responses of Vigna radiata under pot condition was enumerated. The result revealed that the single and dual inoculation of these microbial strains enhances the plant growth in terms of root and shoot length and dry-biomass. The maximum increase in root length (up to 86.57%), shoot length (up to 56.91%), root dry weight (up to 94.42%), and shoot dry weight (up to 56.09%) was observed in response to dual inoculation of Pseudomonas putida with Trichoderma viride compared to uninoculated control. INTRODUCTION: Plant growth promoting rhizobacteria (PGPR) are a group of bacteria that actively colonize plant roots and increase plant growth and yield 1, 2, 3 . The important traits of PGPRs include fixation of atmospheric nitrogen, solubilization of insoluble inorganic phosphates, production of plant hormones, siderophores, bacteriocins etc. These organisms also provide protection to plants against diseases by suppressing deleterious and pathogenic microorganisms 4 . The potential to use PGPR in integrated strategies to reduce Nitrogen and Phosphorus fertilizers offers an appealing research area for those scientists engaged in growth promotion studies in dependable of biological control.

[1]  M. U. Rani,et al.  Screening of rhizobacteria containing plant growth promoting (PGPR) traits in rhizosphere soils and their role in enhancing growth of pigeon pea , 2012 .

[2]  Morris Boris Jacobs,et al.  Handbook Of Microbiology , 2012 .

[3]  Padmavathi Tallapragada,et al.  Phosphate-solubilizing microbes and their occurrence in the rhizospheres of Piper betel in Karnataka, India , 2012 .

[4]  H. Yasmin,et al.  Effect of plant growth promoting rhizobacteria on root morphology of Safflower ( Carthamus tinctorius L.) , 2011 .

[5]  J. Singh,et al.  Exopolysaccharide-Producing Plant Growth-Promoting Rhizobacteria Under Salinity Condition , 2011 .

[6]  Ç. Küçük Inoculation with Rhizobium spp. in kidney bean (Phaseolus vulgaris L.) varieties. , 2011 .

[7]  D. Singh,et al.  Genetic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Rhizospheric Soil of Wheat Under Saline Condition , 2009, Current Microbiology.

[8]  M. Ashrafuzzaman,et al.  Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth , 2009 .

[9]  M. Ahmadzadeh,et al.  Evaluation of fluorescent pseudomonads for plant growth promotion, antifungal activity against Rhizoctonia solani on common bean, and biocontrol potential , 2009 .

[10]  H. Rahmani,et al.  Evaluation of bean (Phaseolus vulgaris) seeds inoculation with Rhizobium phaseoli and plant growth promoting rhizobacteria on yield and yield components. , 2008, Pakistan journal of biological sciences : PJBS.

[11]  P. Trivedi,et al.  Pseudomonas corrugata: A suitable bacterial inoculant for maize grown under rainfed conditions of Himalayan region , 2007 .

[12]  H. Antoun,et al.  Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with pseudomonas putida or Trichoderma atroviride: Possible role of indole acetic acid (IAA) , 2007 .

[13]  I. Kennedy,et al.  Non-symbiotic bacterial diazotrophs in crop-farming systems : can their potential for plant growth promotion be better exploited? , 2004 .

[14]  G. Holguin,et al.  Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003). , 2004, Canadian journal of microbiology.

[15]  M. Arshad,et al.  Screening rhizobacteria for improving the growth, yield, and oil content of canola (Brassica napus L.) , 2004 .

[16]  A. Khalid,et al.  Screening plant growth‐promoting rhizobacteria for improving growth and yield of wheat , 2004, Journal of applied microbiology.

[17]  J. Vessey Plant growth promoting rhizobacteria as biofertilizers , 2003, Plant and Soil.

[18]  M. H. Wonga,et al.  Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial , 2004 .

[19]  N. Dashti,et al.  Application of plant growth-promoting rhizobacteria to soybean (Glycine max [L.] Merr.) increases protein and dry matter yield under short-season conditions , 2004, Plant and Soil.

[20]  K.R.Aneja Experiments in Microbiology, Plant Pathology and Biotechnology , 2003 .

[21]  A. Tripathi,et al.  Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice. , 2001, Journal of biotechnology.

[22]  I. E. García de Salamone,et al.  Cytokinin production by plant growth promoting rhizobacteria and selected mutants. , 2001, Canadian journal of microbiology.

[23]  M. Cheesbrough,et al.  District Laboratory Practice in Tropical Countries, Part 2 , 2006 .

[24]  J. Ladha,et al.  Rhizobia inoculation improves nutrient uptake and growth of lowland rice. , 2000 .

[25]  D. Gould,et al.  The Microbiology Laboratory , 1984, Bio/Technology.

[26]  S. T. Cowan Bergey's Manual of Determinative Bacteriology , 1948, Nature.