Removal of organic substances and oxidation of ammonium nitrogen by a down-flow hanging sponge (DHS) reactor under high salinity conditions.

A down-flow hanging sponge (DHS) reactor, constructed by connecting three identical treatment units in series, was fed with highly saline artificial coke-plant wastewater containing 1400 mg L(-1) of phenol in terms of chemical oxygen demand (COD) and 500 mg-NL(-1) of ammonium nitrogen. The COD was removed by the 1st unit, achieving 92% removal at an average COD loading rate of 3.0 kg-COD m(-3)d(-1) for all units, with oxidation of ammonium nitrogen occurring primarily in the two downstream units. Microbial assays of the different units of the reactor revealed greater numbers of nitrifying bacteria in the 2nd and 3rd units than in the 1st unit, corresponding with the observed ammonium oxidation pattern of the reactor. These findings suggest that a succession of microflora was successfully established along the DHS.

[1]  J. Waterbury,et al.  Nitrospira marina gen. nov. sp. nov.: a chemolithotrophic nitrite-oxidizing bacterium , 1986, Archives of Microbiology.

[2]  K. Schleifer,et al.  The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. , 1999, Systematic and applied microbiology.

[3]  R. Amann,et al.  Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations , 1990, Applied and environmental microbiology.

[4]  L. Kerkhof,et al.  Characterization of bacterial consortia capable of degrading 4-chlorobenzoate and 4-bromobenzoate under denitrifying conditions. , 2002, FEMS microbiology letters.

[5]  M Tandukar,et al.  A low-cost municipal sewage treatment system with a combination of UASB and the "fourth-generation" downflow hanging sponge reactors. , 2005, Water science and technology : a journal of the International Association on Water Pollution Research.

[6]  Hideki Harada,et al.  Diversity, Localization, and Physiological Properties of Filamentous Microbes Belonging to Chloroflexi Subphylum I in Mesophilic and Thermophilic Methanogenic Sludge Granules , 2005, Applied and Environmental Microbiology.

[7]  Yongzhen Peng,et al.  Effects of seawater salinity on nitrite accumulation in short-range nitrification to nitrite as end product. , 2004, Journal of environmental sciences.

[8]  Hideki Harada,et al.  Combination of a UASB reactor and a curtain type DHS (downflow hanging sponge) reactor as a cost-effective sewage treatment system for developing countries , 2000 .

[9]  W. Ludwig,et al.  A new obligately chemolithoautotrophic, nitrite-oxidizing bacterium,Nitrospira moscoviensis sp. nov. and its phylogenetic relationship , 1995, Archives of Microbiology.

[10]  P. Ayoubi,et al.  Constitutive expression of the cloned phenol hydroxylase gene(s) from Alcaligenes eutrophus JMP134 and concomitant trichloroethylene oxidation , 1996, Applied and environmental microbiology.

[11]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[12]  Qian Yi,et al.  Efficacy of pre-treatment methods in the activated sludge removal of refractory compounds in coke-plant wastewater , 1994 .

[13]  Z. Min,et al.  Biological Treatment of Coke-Plant Wastewater for COD and NH3-N Removal , 1991 .

[14]  T. Tokutomi.  Operation of a nitrite-type airlift reactor at low DO concentration. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.

[15]  Y. Watanabe,et al.  Dynamic response of nitrifying activated sludge batch culture to increased chloride concentration. , 2003, Water research.

[16]  S. Stubner.  Enumeration of 16S rDNA of Desulfotomaculum lineage 1 in rice field soil by real-time PCR with SybrGreen detection. , 2002, Journal of microbiological methods.

[17]  S. Goodison,et al.  16S ribosomal DNA amplification for phylogenetic study , 1991, Journal of bacteriology.

[18]  H J Lubberding,et al.  Long term effects of salt on activity, population structure and floc characteristics in enriched bacterial cultures of nitrifiers. , 2006, Water research.

[19]  S Uemura,et al.  Removal of indigenous coliphages and fecal coliforms by a novel sewage treatment system consisting of UASB and DHS units. , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[20]  S. Parent,et al.  Nitratireductor aquibiodomus gen. nov., sp. nov., a novel alpha-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). , 2004, International journal of systematic and evolutionary microbiology.