Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer.

A new biomolecular quantitation method, nanoparticle-mediated photothermal bioassay, using a common thermometer as the signal reader was developed. Using an immunoassay as a proof of concept, iron oxide nanoparticles (NPs) captured in the sandwich-type assay system were transformed into a near-infrared (NIR) laser-driven photothermal agent, Prussian blue (PB) NPs, which acted as a photothermal probe to convert the assay signal into heat through the photothermal effect, thus allowing sensitive biomolecular quantitation using a thermometer. This is the first report of biomolecular quantitation using a thermometer and also serves as the first attempt to introduce the nanoparticle-mediated photothermal effect for bioassays.

[1]  H. Choi,et al.  In vivo near-infrared mediated tumor destruction by photothermal effect of carbon nanotubes. , 2009, ACS Nano.

[2]  L. Gervais,et al.  Microfluidic Chips for Point‐of‐Care Immunodiagnostics , 2011, Advanced materials.

[3]  Xingyu Jiang,et al.  Copper-mediated amplification allows readout of immunoassays by the naked eye. , 2011, Angewandte Chemie.

[4]  Xiaolei Zuo,et al.  Ultrasensitive electrochemical detection of prostate-specific antigen by using antibodies anchored on a DNA nanostructural scaffold. , 2014, Analytical chemistry.

[5]  Mark A. Griswold,et al.  Dual purpose Prussian blue nanoparticles for cellular imaging and drug delivery: a new generation of T1-weighted MRI contrast and small molecule delivery agents , 2010 .

[6]  X. Qu,et al.  New Horizons for Diagnostics and Therapeutic Applications of Graphene and Graphene Oxide , 2013, Advanced materials.

[7]  Christopher G. Khoury,et al.  Plasmonic nanoprobes: from chemical sensing to medical diagnostics and therapy. , 2013, Nanoscale.

[8]  Hongyuan Chen,et al.  A dual-functional electrochemical biosensor for the detection of prostate specific antigen and telomerase activity. , 2013, Chemical communications.

[9]  Srirang Manohar,et al.  Light interactions with gold nanorods and cells: implications for photothermal nanotherapeutics. , 2011, Nano letters.

[10]  Mingdong Huang,et al.  Dissolution-enhanced luminescent bioassay based on inorganic lanthanide nanoparticles. , 2014, Angewandte Chemie.

[11]  Guanghui Ma,et al.  Theranostic Gold Nanomicelles made from Biocompatible Comb‐like Polymers for Thermochemotherapy and Multifunctional Imaging with Rapid Clearance , 2015, Advanced materials.

[12]  Y. Yamauchi,et al.  Synthesis of Prussian blue nanoparticles with a hollow interior by controlled chemical etching. , 2012, Angewandte Chemie.

[13]  Feng Xu,et al.  Low-cost bioanalysis on paper-based and its hybrid microfluidic platforms. , 2015, Talanta.

[14]  Hongyuan Chen,et al.  Synthesis and Characterization of Prussian Blue Modified Magnetite Nanoparticles and Its Application to the Electrocatalytic Reduction of H2O2 , 2005 .

[15]  Y. Ning,et al.  Near-infrared light-responsive supramolecular nanovalve based on mesoporous silica-coated gold nanorods , 2014 .

[16]  J. G. Solé,et al.  Nanoparticles for photothermal therapies. , 2014, Nanoscale.

[17]  XiuJun Li,et al.  A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection. , 2013, Lab on a chip.

[18]  Z. Dai,et al.  Chitosan stabilized Prussian blue nanoparticles for photothermally enhanced gene delivery. , 2014, Colloids and surfaces. B, Biointerfaces.

[19]  Xiaogang Qu,et al.  Hydrophobic Anticancer Drug Delivery by a 980 nm Laser‐Driven Photothermal Vehicle for Efficient Synergistic Therapy of Cancer Cells In Vivo , 2013, Advanced materials.

[20]  X. Qu,et al.  Recent progress in nanosensors for sensitive detection of biomolecules. , 2013, Nanoscale.

[21]  Joonhyung Lee,et al.  Two-dimensional Layered MoS2 Biosensors Enable Highly Sensitive Detection of Biomolecules , 2014, Scientific Reports.

[22]  Z. Dai,et al.  Magnetic Prussian blue nanoparticles for targeted photothermal therapy under magnetic resonance imaging guidance. , 2014, Bioconjugate chemistry.

[23]  Kai Yang,et al.  In Vitro and In Vivo Near‐Infrared Photothermal Therapy of Cancer Using Polypyrrole Organic Nanoparticles , 2012, Advanced materials.

[24]  Gabriel A Kwong,et al.  Point-of-care diagnostics for noncommunicable diseases using synthetic urinary biomarkers and paper microfluidics , 2014, Proceedings of the National Academy of Sciences.

[25]  Feng Xu,et al.  Biomarker detection for disease diagnosis using cost-effective microfluidic platforms. , 2015, The Analyst.

[26]  Zhuang Liu,et al.  PEGylated Prussian blue nanocubes as a theranostic agent for simultaneous cancer imaging and photothermal therapy. , 2014, Biomaterials.

[27]  M. Dou,et al.  A Versatile PDMS/Paper Hybrid Microfluidic Platform for Sensitive Infectious Disease Diagnosis , 2014, Analytical chemistry.

[28]  Xiuli Yue,et al.  Prussian blue nanoparticles operate as a new generation of photothermal ablation agents for cancer therapy. , 2012, Chemical communications.

[29]  John T. Wei,et al.  Beyond PSA: The Next Generation of Prostate Cancer Biomarkers , 2012, Science Translational Medicine.

[30]  Rujia Zou,et al.  Cu7.2S4 nanocrystals: a novel photothermal agent with a 56.7% photothermal conversion efficiency for photothermal therapy of cancer cells. , 2014, Nanoscale.

[31]  Meng Li,et al.  Battery-triggered ultrasensitive electrochemiluminescence detection on microfluidic paper-based immunodevice based on dual-signal amplification strategy. , 2013, Analytica chimica acta.

[32]  James F Rusling,et al.  Electrochemical immunosensors for antibodies to peanut allergen ara h2 using gold nanoparticle-peptide films. , 2010, Analytical chemistry.

[33]  Dianping Tang,et al.  Enhanced Colorimetric Immunoassay Accompanying with Enzyme Cascade Amplification Strategy for Ultrasensitive Detection of Low-Abundance Protein , 2014, Scientific Reports.

[34]  Hashem Akhavan-Tafti,et al.  A homogeneous chemiluminescent immunoassay method. , 2013, Journal of the American Chemical Society.

[35]  Jeong-Woo Choi,et al.  A novel Au-nanoparticle biosensor for the rapid and simple detection of PSA using a sequence-specific peptide cleavage reaction. , 2013, Biosensors & bioelectronics.