In vivo, transcutaneous glucose sensing using surface-enhanced spatially offset Raman spectroscopy: multiple rats, improved hypoglycemic accuracy, low incident power, and continuous monitoring for greater than 17 days.

This paper presents the latest progress on quantitative, in vivo, transcutaneous glucose sensing using surface enhanced spatially offset Raman spectroscopy (SESORS). Silver film over nanosphere (AgFON) surfaces were functionalized with a mixed self-assembled monolayer (SAM) and implanted subcutaneously in Sprague-Dawley rats. The glucose concentration was monitored in the interstitial fluid of six separate rats. The results demonstrated excellent accuracy and consistency. Remarkably, the root-mean-square error of calibration (RMSEC) (3.6 mg/dL) and the root-mean-square error of prediction (RMSEP) (13.7 mg/dL) for low glucose concentration (<80 mg/dL) is lower than the current International Organization Standard (ISO/DIS 15197) requirements. Additionally, our sensor demonstrated functionality up 17 days after implantation, including 12 days under the laser safety level for human skin exposure with only one time calibration. Therefore, our SERS based sensor shows promise for the challenge of reliable continuous glucose sensing systems for optimal glycemic control.

[1]  Hyungsoon Im,et al.  Recent progress in SERS biosensing. , 2011, Physical chemistry chemical physics : PCCP.

[2]  George C. Schatz,et al.  Correlated Structure and Optical Property Studies of Plasmonic Nanoparticles , 2011 .

[3]  R. Dasari,et al.  Investigation of the specificity of Raman spectroscopy in non-invasive blood glucose measurements , 2011, Analytical and bioanalytical chemistry.

[4]  Chuanbin Mao,et al.  Bio-imaging, detection and analysis by using nanostructures as SERS substrates. , 2011, Journal of materials chemistry.

[5]  G. Lloyd,et al.  Surface enhanced spatially offset Raman spectroscopic (SESORS) imaging – the next dimension , 2011 .

[6]  Richard P Van Duyne,et al.  Transcutaneous glucose sensing by surface-enhanced spatially offset Raman spectroscopy in a rat model. , 2010, Analytical chemistry.

[7]  Bruce Buckingham,et al.  Toward closing the loop: an update on insulin pumps and continuous glucose monitoring systems. , 2010, Endocrinology and metabolism clinics of North America.

[8]  George C Schatz,et al.  Structure-activity relationships in gold nanoparticle dimers and trimers for surface-enhanced Raman spectroscopy. , 2010, Journal of the American Chemical Society.

[9]  Sanjay Mehrotra,et al.  Prediction range estimation from noisy Raman spectra with robust optimization. , 2010, The Analyst.

[10]  Duncan Graham,et al.  Prospects of deep Raman spectroscopy for noninvasive detection of conjugated surface enhanced resonance Raman scattering nanoparticles buried within 25 mm of mammalian tissue. , 2010, Analytical chemistry.

[11]  R. Busch,et al.  Accuracy and precision of four value-added blood glucose meters: the Abbott Optium, the DDI Prodigy, the HDI True Track, and the HypoGuard Assure Pro. , 2009, Diabetes technology & therapeutics.

[12]  Sanjiv S. Gambhir,et al.  Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopy , 2009, Proceedings of the National Academy of Sciences.

[13]  Y. Ozaki,et al.  Development of a heat-induced surface-enhanced Raman scattering sensing method for rapid detection of glutathione in aqueous solutions. , 2009, Analytical chemistry.

[14]  C. Toumazou,et al.  Glucose sensors: a review of current and emerging technology , 2009, Diabetic medicine : a journal of the British Diabetic Association.

[15]  Francesco De Angelis,et al.  Nano-patterned SERS substrate: application for protein analysis vs. temperature. , 2009, Biosensors & bioelectronics.

[16]  G. Ward,et al.  The minimal model of glucose disposal in the analysis of glucose effectiveness: importance of early insulin data. , 2009, Diabetes technology & therapeutics.

[17]  Olga Lyandres,et al.  Progress toward an in vivo surface-enhanced Raman spectroscopy glucose sensor. , 2008, Diabetes technology & therapeutics.

[18]  Alaaldin M. Alkilany,et al.  Gold nanoparticles in biology: beyond toxicity to cellular imaging. , 2008, Accounts of chemical research.

[19]  Nastassja A. Lewinski,et al.  Cytotoxicity of nanoparticles. , 2008, Small.

[20]  Pavel Matousek,et al.  Subsurface probing of calcifications with spatially offset Raman spectroscopy (SORS): future possibilities for the diagnosis of breast cancer. , 2007, The Analyst.

[21]  V. R. Kondepati,et al.  Recent progress in analytical instrumentation for glycemic control in diabetic and critically ill patients , 2007, Analytical and bioanalytical chemistry.

[22]  Vicki Stone,et al.  Toxicology of nanoparticles: A historical perspective , 2007 .

[23]  D. A. Stuart,et al.  In vivo glucose measurement by surface-enhanced Raman spectroscopy. , 2006, Analytical chemistry.

[24]  Jing Zhao,et al.  Ultrastable substrates for surface-enhanced Raman spectroscopy: Al2O3 overlayers fabricated by atomic layer deposition yield improved anthrax biomarker detection. , 2006, Journal of the American Chemical Society.

[25]  Pavel Matousek,et al.  Noninvasive Raman Spectroscopy of Human Tissue in vivo , 2006, Applied spectroscopy.

[26]  Bruce A Buckingham,et al.  Evaluation of factors affecting CGMS calibration. , 2006, Diabetes technology & therapeutics.

[27]  A. Goodship,et al.  Numerical Simulations of Subsurface Probing in Diffusely Scattering Media Using Spatially Offset Raman Spectroscopy , 2005, Applied spectroscopy.

[28]  W. Clarke.  The original Clarke Error Grid Analysis (EGA). , 2005, Diabetes technology & therapeutics.

[29]  Olga Lyandres,et al.  Real-time glucose sensing by surface-enhanced Raman spectroscopy in bovine plasma facilitated by a mixed decanethiol/mercaptohexanol partition layer. , 2005, Analytical chemistry.

[30]  D. A. Stuart,et al.  Glucose sensing using near-infrared surface-enhanced Raman spectroscopy: gold surfaces, 10-day stability, and improved accuracy. , 2005, Analytical chemistry.

[31]  William F. Finney,et al.  Subsurface Probing in Diffusely Scattering Media Using Spatially Offset Raman Spectroscopy , 2005, Applied spectroscopy.

[32]  Peter G Jacobs,et al.  Feasibility of continuous long-term glucose monitoring from a subcutaneous glucose sensor in humans. , 2004, Diabetes technology & therapeutics.

[33]  Eric Renard,et al.  Implantable closed-loop glucose-sensing and insulin delivery: the future for insulin pump therapy. , 2002, Current opinion in pharmacology.

[34]  P. Cryer,et al.  Hypoglycaemia: The limiting factor in the glycaemic management of Type I and Type II Diabetes* , 2002, Diabetologia.

[35]  F. Kaufman,et al.  A pilot study of the continuous glucose monitoring system: clinical decisions and glycemic control after its use in pediatric type 1 diabetic subjects. , 2001, Diabetes care.

[36]  Ramasamy Manoharan,et al.  Detection and identification of a single DNA base molecule using surface-enhanced Raman scattering (SERS) , 1998 .

[37]  V. Basevi.  Standards of Medical Care in Diabetes—2011 , 2011, Diabetes Care.

[38]  D. Cox,et al.  Evaluating Clinical Accuracy of Systems for Self-Monitoring of Blood Glucose , 1987, Diabetes Care.