In vitro regeneration study in lablab bean and dolichos bean (Lablab purpureus (L). Sweet) Genotypes

The current investigation was carried out to study in vitro regeneration potential in Lablab bean genotypes and Dolichos bean Cv. (Konkan Bhushan). In surface sterilization, treatment with 70% alcohol for 5 minute followed by HgCl2 (0.1%) for 10 minutes was found the most effective in establishing aseptic cultures (97.22%). Mature embryo axis with single cotyledon (MEASC) explant on medium MS+ 6 mg/l BAP+ 0.5 mg/l NAA responded well for shoot induction frequency, multiple shoot induction in Dolichos bean genotype ‘Konkan Bhushan’. The medium MS+ 0.5mg/l BAP+ 0.2 mg/l GA3 was found better for elongation of in vitro regenerated shoots. The genotype ‘Konkan Bhushan’ rooted also earliest in 9.30 days on medium MS+ 1.0 mg/l IBA+ 2% sucrose with highest root induction frequency (79.51%) and maximum roots per shoot (5.04). During hardening, the potting mixture of soil, vermiculite and sand in 1:1:1 showed the highest survival rate.

[1]  Haque,et al.  In vitro Regeneration of Mungbean ( Vigna radiata L.) from Different Explants , 2013 .

[2]  Idris,et al.  Simple, effective and economical explant-surface sterilization protocol for cowpea, rice and sorghum seeds , 2009 .

[3]  J. Iqbal,et al.  MULTIPLE SHOOT FORMATION FROM DIFFERENT EXPLANTS OF CHICK PEA (CICER ARIETINUM L.) , 2007 .

[4]  V. Kale.  Genotypic differences for callus induction and plantlet regeneration in field bean [Lablab purpureus (L.) Sweet] , 2004 .

[5]  K. Sharma,et al.  An efficient protocol for shoot regeneration and genetic transformation of pigeonpea [Cajanus cajan (L.) Millsp.] using leaf explants , 2003, Plant Cell Reports.

[6]  L. Chand,et al.  Plantlet regeneration from decapitated embryonic axes of pigeonpea [Cajanus cajan (L.) Millsp.]. , 1999, Indian journal of experimental biology.

[7]  N. Geetha,et al.  High frequency induction of multiple shoots and plant regeneration from seedling explants of pigeonpea (Cajanus cajan l.) , 1998 .

[8]  K. Krishnamurthy,et al.  Plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp.] by organogenesis , 1998, Plant Cell Reports.

[9]  G. Franklin,et al.  MULTIPLE SHOOT INDUCTION AND REGENERATION OF PIGEON PEA (CAJANUS CAJAN (L.) MILLSP) CV. VAMBAN 1 FROM APICAL AND AXILLARY MERISTEM , 1998 .

[10]  P. Saxena,et al.  Thidiazuron induces high-frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris) , 1992 .

[11]  J. P. Moss,et al.  Tissue culture and regeneration in legumes. , 1992 .

[12]  Ashwani Kumar,et al.  Plantlet regeneration from different callus cultures of pigeonpea (Cajanus cajan L.) , 1983 .

[13]  F. Skoog,et al.  A revised medium for the growth and bioassay with tobacco tissue culture , 1962 .