Isolation and structures of biselyngbyasides B, C, and D from the marine cyanobacterium Lyngbya sp., and the biological activities of biselyngbyasides

Abstract Bioassay-guided fractionation of the marine cyanobacterium Lyngbya sp. led to the isolation of biselyngbyasides B ( 3 ), C ( 4 ), and D ( 5 ), novel analogs of biselyngbyaside ( 1 ) and biselyngbyolide A ( 2 ). The gross structures of 3 – 5 were determined by NMR spectral analyses, and their stereochemistries were established based on NOESY spectra and CD data. Biselyngbyasides ( 1 – 3 ) showed growth-inhibitory activity and apoptosis-inducing activity against both HeLa S 3 cells and HL60 cells. The fura-2 method revealed that biselyngbyasides ( 1 – 3 ) increased the cytosolic Ca 2+ concentration in HeLa S 3 cells.

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