Treatment of breast cancer through the application of irreversible electroporation using a novel minimally invasive single needle electrode

Irreversible electroporation (IRE) is a therapeutic technology for the ablation of soft tissues using electrodes to deliver intense but short electric pulses across a cell membrane, creating nanopores that lead to cell death. This phenomenon only affects the cell membrane, leaving the extracellular matrix and sensitive structures intact, making it a promising technique for the treatment many types of tumors. In this paper, we present the first in vivo study to achieve tumor regression using a translatable, clinically relevant single needle electrode for treatment administration. Numerical models of the electric field distribution for the protocol used suggest that a 1000 V/cm field threshold is sufficient to treat a tumor, and that the electric field distribution will slightly decrease if the same protocol were used on a tumor deep seated within a human breast. Tumor regression was observed in 5 out of 7 MDA-MB231 human mammary tumors orthotopically implanted in female Nu/Nu mice, with continued growth in controls.

[1]  S. Singletary,et al.  Radiofrequency ablation of early-stage invasive breast tumors: an overview. , 2002, Cancer journal.

[2]  Boris Rubinsky,et al.  Irreversible Electroporation: A New Ablation Modality — Clinical Implications , 2007, Technology in cancer research & treatment.

[3]  Paulo A. Garcia,et al.  NON-THERMAL IRREVERSIBLE ELECTROPORATION , 2010 .

[4]  M. Gnant,et al.  Breast-Conserving Therapy for Centrally Located Breast Cancer , 2008, Annals of surgery.

[5]  T. Franquet,et al.  Spiculated lesions of the breast: mammographic-pathologic correlation. , 1993, Radiographics : a review publication of the Radiological Society of North America, Inc.

[6]  Robert C. Wolpert,et al.  A Review of the , 1985 .

[7]  P. Littrup,et al.  Cryotherapy for breast cancer: a feasibility study without excision. , 2009, Journal of vascular and interventional radiology : JVIR.

[8]  Rafael V. Davalos,et al.  The Feasibility of Irreversible Electroporation for the Treatment of Breast Cancer and Other Heterogeneous Systems , 2009, Annals of Biomedical Engineering.

[9]  Francis A. Duck,et al.  Physical properties of tissue : a comprehensive reference book , 1990 .

[10]  A. Baldi,et al.  Electroporation in Laboratory and Clinical Investigations , 2011 .

[11]  B Fisher,et al.  Eight-year results of a randomized clinical trial comparing total mastectomy and lumpectomy with or without irradiation in the treatment of breast cancer. , 1989, The New England journal of medicine.

[12]  Stuchly,et al.  Dielectric properties of breast carcinoma and the surrounding tissues , 1988, IEEE Transactions on Biomedical Engineering.

[13]  D. Origgi,et al.  Magnetic resonance mammography in the evaluation of recurrence at the prior lumpectomy site after conservative surgery and radiotherapy , 2006, Breast Cancer Research.

[14]  Boris Rubinsky,et al.  Irreversible Electroporation: Implications for Prostate Ablation , 2007, Technology in cancer research & treatment.

[15]  Boris Rubinsky,et al.  Electrical field and temperature model of nonthermal irreversible electroporation in heterogeneous tissues. , 2009, Journal of biomechanical engineering.

[16]  B. Rubinsky,et al.  Tissue Ablation with Irreversible Electroporation , 2005, Annals of Biomedical Engineering.

[17]  Jon F Edd,et al.  Mathematical Modeling of Irreversible Electroporation for Treatment Planning , 2007, Technology in cancer research & treatment.

[18]  J. Weaver,et al.  Theory of electroporation: A review , 1996 .

[19]  R. Simmons,et al.  Cryoablation of Early-Stage Breast Cancer: Work-in-Progress Report of a Multi-Institutional Trial , 2004, Annals of Surgical Oncology.

[20]  Boris Rubinsky,et al.  Temperature considerations during irreversible electroporation , 2008 .

[21]  Atam P. Dhawan,et al.  Concise Matrix Analysis of Point-Based Prostate Targeting for Intensity Modulated Radiation Therapy , 2007, Technology in cancer research & treatment.

[22]  I. Fentiman,et al.  Radiofrequency ablation in the treatment of primary breast cancer: no surgical redundancies yet , 2008, International journal of clinical practice.

[23]  Boris Rubinsky,et al.  Tumor Ablation with Irreversible Electroporation , 2007, PloS one.

[24]  S. Orlowski,et al.  Mechanisms of electrochemotherapy. , 1999, Advanced drug delivery reviews.

[25]  M. Goldfarb,et al.  Oncologic and Aesthetic Results Following Breast‐Conserving Therapy with 0.5 cm Margins in 100 Consecutive Patients , 2006, The breast journal.

[26]  A. Belldegrun,et al.  The effects of intentional cryoablation and radio frequency ablation of renal tissue involving the collecting system in a porcine model. , 2005, The Journal of urology.

[27]  J. Hinshaw,et al.  Cryoablation for liver cancer. , 2007, Techniques in vascular and interventional radiology.

[28]  Boris Rubinsky,et al.  In vivo results of a new focal tissue ablation technique: irreversible electroporation , 2006, IEEE Transactions on Biomedical Engineering.