Computational analysis of fluorescence induction curves in intact spinach leaves treated at different pH

Effects of change in pH have been investigated on spinach leaf discs by measuring fluorescence induction kinetics using plant efficiency analyzer (PEA). On the basis of computational analysis of the results, we have reported that acidic pH causes a significant inhibition of the donor and the acceptor side of PS II. Energy flux models have been presented using the software Biolyzer HP 3. Effects of pH were investigated on the antenna size heterogeneity of PS II and a relative change in the proportions of α, β, and γ centers was observed.

[1]  S. Mathur,et al.  Evidence that pH can drive state transitions in isolated thylakoid membranes from spinach , 2010, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[2]  K Maxwell,et al.  Chlorophyll fluorescence--a practical guide. , 2000, Journal of experimental botany.

[3]  Xin-Guang Zhu,et al.  Chlorophyll a fluorescence induction kinetics in leaves predicted from a model describing each discrete step of excitation energy and electron transfer associated with Photosystem II , 2005, Planta.

[4]  J. Anderson,et al.  Localization of different photosystems in separate regions of chloroplast membranes. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Rabek,et al.  Photochemistry and photophysics , 1990 .

[6]  R. Park.  Advances in photosynthesis , 1962 .

[7]  A. Melis,et al.  Dynamics of photosynthetic membrane composition and function , 1991 .

[8]  J. Briantais,et al.  Heat stress induces in leaves an increase of the minimum level of chlorophyll fluorescence, Fo: A time-resolved analysis , 1996, Photosynthesis Research.

[9]  S. Mathur,et al.  Analysis of elevated temperature-induced inhibition of photosystem II using chlorophyll a fluorescence induction kinetics in wheat leaves (Triticum aestivum). , 2011, Plant biology.

[10]  D. Kramer,et al.  How acidic is the lumen? , 1999, Photosynthesis Research.

[11]  B. Hsu,et al.  A study on the fluorescence induction curve of the DCMU-poisoned chloroplast , 1991 .

[12]  M. Marietta,et al.  Electron‐dependent competition between plastoquinone and inhibitors for binding to photosystem II , 1981 .

[13]  A. B. Hope,et al.  Effects of pH on the kinetics of redox reactions in and around the cytochromebf complex in an isolated system , 1994, Photosynthesis Research.

[14]  J. Lavergne,et al.  Stabilization of charge separation and photochemical misses in photosystem II. , 1998, Biochemistry.

[15]  R. Strasser,et al.  Analysis of the Chlorophyll a Fluorescence Transient , 2004 .

[16]  A. Srivastava,et al.  POLYPHASIC CHLOROPHYLL a FLUORESCENCE TRANSIENT IN PLANTS AND CYANOBACTERIA * , 1995 .

[17]  D. Kramer,et al.  Balancing the central roles of the thylakoid proton gradient. , 2003, Trends in plant science.

[18]  B. Hsu,et al.  A Method for Analysis of Fluorescence Induction Curve From Dcmu-Poisoned Chloroplasts , 1989 .

[19]  R. Strasser,et al.  In intact leaves, the maximum fluorescence level (F(M)) is independent of the redox state of the plastoquinone pool: a DCMU-inhibition study. , 2005, Biochimica et biophysica acta.

[20]  R. Strasser,et al.  Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. , 2010, Biochimica et biophysica acta.

[21]  E. Bergantino,et al.  Light- and pH-dependent structural changes in the PsbS subunit of photosystem II , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[22]  D. Kaftan,et al.  Flash fluorescence induction: a novel method to study regulation of Photosystem II , 1999 .

[23]  J. Nauš,et al.  Determination of the antenna heterogeneity of Photosystem II by direct simultaneous fitting of several fluorescence rise curves measured with DCMU at different light intensities , 2004, Photosynthesis Research.

[24]  A. Melis,et al.  Supramolecular Assembly and Function of Subunits Associated with the Chlorophyll a-b Light-Harvesting Complex II (LHC II) in Soybean Chloroplasts , 1989 .

[25]  S. Mathur,et al.  Chlorophyll a fluorescence study revealing effects of high salt stress on Photosystem II in wheat leaves. , 2010, Plant physiology and biochemistry : PPB.

[26]  K. Niyogi,et al.  Non-photochemical quenching. A response to excess light energy. , 2001, Plant physiology.