Improved applicability and robustness of fast cryo-electron tomography data acquisition

Graphical abstract

[1]  Radostin Danev,et al.  Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions. , 2019, Trends in biochemical sciences.

[2]  Isabella Haberbosch,et al.  Software tools for automated transmission electron microscopy , 2018, Nature Methods.

[3]  G. Zanetti,et al.  Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape , 2018, Nature Communications.

[4]  Min Xu,et al.  De Novo Structural Pattern Mining in Cellular Electron Cryotomograms. , 2015, Structure.

[5]  Wei Dai,et al.  Convolutional Neural Networks for Automated Annotation of Cellular Cryo-Electron Tomograms , 2017, Nature Methods.

[6]  G. Jensen,et al.  Marine Tubeworm Metamorphosis Induced by Arrays of Bacterial Phage Tail–Like Structures , 2014, Science.

[7]  F. Schur,et al.  Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging. , 2019, Current opinion in structural biology.

[8]  F. Förster,et al.  Subtomogram analysis using the Volta phase plate. , 2017, Journal of structural biology.

[9]  J. Medeiros,et al.  In Situ Imaging of Bacterial Secretion Systems by Electron Cryotomography. , 2017, Methods in molecular biology.

[10]  Yuxiang Chen,et al.  PyTom: a python-based toolbox for localization of macromolecules in cryo-electron tomograms and subtomogram analysis. , 2012, Journal of structural biology.

[11]  J. Briggs,et al.  High-resolution structures of HIV-1 Gag cleavage mutants determine structural switch for virus maturation , 2018, Proceedings of the National Academy of Sciences.

[12]  A. Verkleij,et al.  Automated high‐throughput electron tomography by pre‐calibration of image shifts , 2002, Journal of microscopy.

[13]  Nikolaus Grigorieff,et al.  Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6 , 2015, eLife.

[14]  David N Mastronarde,et al.  Automated electron microscope tomography using robust prediction of specimen movements. , 2005, Journal of structural biology.

[15]  D. Mastronarde.  Dual-axis tomography: an approach with alignment methods that preserve resolution. , 1997, Journal of structural biology.

[16]  K. D. van der Mast,et al.  An autofocus method for a TEM , 1987 .

[17]  Benjamin A Himes,et al.  emClarity: Software for High Resolution Cryo-electron Tomography and Sub-tomogram Averaging , 2018, Nature Methods.

[18]  Kai Zhang,et al.  Gctf: Real-time CTF determination and correction , 2015, bioRxiv.

[19]  Daniel Castaño-Díez,et al.  Dynamo: a flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments. , 2012, Journal of structural biology.

[20]  J. Briggs,et al.  An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation , 2016, Science.

[21]  Grant J Jensen,et al.  Rapid tilt-series acquisition for electron cryotomography. , 2019, Journal of structural biology.

[22]  Hanspeter Winkler,et al.  3D reconstruction and processing of volumetric data in cryo-electron tomography. , 2007, Journal of structural biology.

[23]  J. Briggs.  Structural biology in situ--the potential of subtomogram averaging. , 2013, Current opinion in structural biology.

[24]  J. Briggs,et al.  Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging , 2017, Journal of structural biology.

[25]  D. Agard,et al.  An improved strategy for automated electron microscopic tomography. , 2004, Journal of structural biology.