In cell scalaradial interactome profiling using a bio-orthogonal clickable probe.

A bio-orthogonal click-chemistry procedure was developed to allow the in cell interactome profiling of scalaradial, an anti-inflammatory marine natural product. The results were validated through the application of the classical in vitro chemical proteomics and several bio-physical methods; peroxiredoxins, 14-3-3 isoforms and proteasomes were recognized as main scalaradial targets.

[1]  Agostino Casapullo,et al.  Chemical proteomics-driven discovery of oleocanthal as an Hsp90 inhibitor. , 2013, Chemical communications.

[2]  J. Balsinde,et al.  Group V Phospholipase A2-Derived Lysophosphatidylcholine Mediates Cyclooxygenase-2 Induction in Lipopolysaccharide-Stimulated Macrophages1 , 2007, The Journal of Immunology.

[3]  L. Gomez‐Paloma,et al.  The inactivation of phospholipase A2 by scalaradial: a biomimetic study by electrospray mass spectrometry. , 2005, Rapid communications in mass spectrometry : RCM.

[4]  T. Hashimoto,et al.  1,3-Dipolar Cycloaddition , 2010 .

[5]  Jennifer A. Prescher,et al.  Chemistry in living systems , 2005, Nature chemical biology.

[6]  R. Levy,et al.  Phospholipase A2 has a role in proliferation but not in differentiation of HL-60 cells. , 1997, Biochimica et biophysica acta.

[7]  R. Riccio,et al.  Chemical proteomics discloses petrosapongiolide M, an antiinflammatory marine sesterterpene, as a proteasome inhibitor. , 2010, Angewandte Chemie.

[8]  G. Superti-Furga,et al.  Target profiling of small molecules by chemical proteomics. , 2009, Nature chemical biology.

[9]  Anna E Speers,et al.  Profiling enzyme activities in vivo using click chemistry methods. , 2004, Chemistry & biology.

[10]  S. Kitamura,et al.  Reductive Metabolism of an α,β-Ketoalkyne, 4-Phenyl-3-Butyn-2-One, by Rat Liver Preparations , 2002 .

[11]  A. Zampella,et al.  Chemical Proteomics Reveals Heat Shock Protein 60 To Be the Main Cellular Target of the Marine Bioactive Sesterterpene Suvanine , 2012, Chembiochem : a European journal of chemical biology.

[12]  Yili Xie,et al.  Scalaradial Inhibition of Epidermal Growth Factor Receptor-Mediated Akt Phosphorylation Is Independent of Secretory Phospholipase A2 , 2005, Journal of Pharmacology and Experimental Therapeutics.

[13]  R. Weissleder,et al.  Bioorthogonal approach to identify unsuspected drug targets in live cells. , 2013, Angewandte Chemie.

[14]  A. Zampella,et al.  Heteronemin, a marine sponge terpenoid, targets TDP-43, a key factor in several neurodegenerative disorders. , 2014, Chemical communications.

[15]  J. Mestres,et al.  On the origins of drug polypharmacology , 2013 .

[16]  G. Cimino,et al.  12-epi-scalarin and 12-epi-deoxoscalarin, Sesterterpenes from the sponge Spongia nitens. , 1977, Journal of the Chemical Society. Perkin transactions 1.

[17]  L. Marnett,et al.  Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives. , 2008, Chemical research in toxicology.

[18]  M. Mann,et al.  In-gel digestion for mass spectrometric characterization of proteins and proteomes , 2006, Nature Protocols.

[19]  H. Kolb,et al.  The growing impact of click chemistry on drug discovery. , 2003, Drug discovery today.

[20]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[21]  H. Osada,et al.  Cleavable linker for photo-cross-linked small-molecule affinity matrix. , 2010, Bioconjugate chemistry.

[22]  Claudio N Cavasotto,et al.  Scalaradial, a Dialdehyde‐Containing Marine Metabolite That Causes an Unexpected Noncovalent PLA2 Inactivation , 2007, Chembiochem : a European journal of chemical biology.

[23]  M. Maiuri,et al.  Cacospongionolide and Scalaradial, Two Marine Sesterterpenoids as Potent Apoptosis-Inducing Factors in Human Carcinoma Cell Lines , 2012, PloS one.

[24]  G. Bifulco,et al.  The Inactivation Mechanism of Human Group IIA Phospholipase A2 by Scalaradial , 2012, Chembiochem : a European journal of chemical biology.