Effects of Minor Components of Crude Vegetable Oil on the Enzymatic Method to Analyze Positional Fatty Acid Distributions in Triacylglycerols withCandida antarctica Lipase B.

Crude soybean and rapeseed oils were subjected to the method to determine FA distributions in TAG using Candida antarctica lipase B, giving similar results to those for refined oils. Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selective transesterification by the lipase and FA compositional analysis of the resulting 2-MAG fraction significantly. Phospholipids were confirmed not to contaminate the 2-MAG fraction. Oxidized soybean oil with a PV of 10 meq/kg also gave similar results to the ones for refined oil. The method was confirmed to be applicable for crude oils and oxidized oils with a PV smaller than 10 meq/kg without prior purification of TAG.

[1]  Y. Endo.  Analytical Methods to Evaluate the Quality of Edible Fats and Oils: The JOCS Standard Methods for Analysis of Fats, Oils and Related Materials (2013) and Advanced Methods. , 2018, Journal of oleo science.

[2]  Yomi Watanabe,et al.  The Collaborative Study on the Enzymatic Analysis of Positional Distribution of Short- and Medium-chain Fatty Acids in Milk Fat Using Immobilized Candida antarctica Lipase B. , 2016, Journal of oleo science.

[3]  Guolong Yang,et al.  Lysophosphatidylcholine synthesis by lipase-catalyzed ethanolysis. , 2015, Journal of oleo science.

[4]  Yomi Watanabe,et al.  Enzymatic Analysis of Positional Fatty Acid Distributions in Triacylglycerols by 1(3)-Selective Transesterification with Candida antarctica Lipase B: a Collaborative Study. , 2015, Journal of oleo science.

[5]  Yomi Watanabe,et al.  Regiospecific Distribution of trans-Octadecenoic Acid Positional Isomers in Triacylglycerols of Partially Hydrogenated Vegetable Oil and Ruminant Fat. , 2015, Journal of oleo science.

[6]  Yomi Watanabe,et al.  Enzymatic Analysis of Positional Distribution of Fatty Acids in Solid Fat by 1,3-Selective Transesterification with Candida antarctica Lipase B , 2014 .

[7]  Yomi Watanabe,et al.  Control of the regiospecificity of Candida antarctica lipase by polarity. , 2009, New biotechnology.

[8]  Yomi Watanabe,et al.  Production of FAME from acid oil model using immobilized Candida antarctica lipase , 2005 .

[9]  Yomi Watanabe,et al.  Regiospecific analysis by ethanolysis of oil with immobilized Candida antarctica lipase , 2003, Lipids.

[10]  F. Ulberth,et al.  Flame-ionization detector response to methyl, ethyl, propyl, and butyl esters of fatty acids , 1999 .

[11]  L. Jørgensen,et al.  Positional distribution of ω3 Fatty acids in marine lipid triacylglycerols by high-resolution13C nuclear magnetic resonance spectroscopy , 1995 .