[13] Cell culture characterization: Monitoring for cell identification☆

Publisher Summary This chapter discusses cell culture characterization. Cells are passaged, co-cultivated, transformed, and/or transplanted for numerous investigative purposes. Monitoring also is necessary when cultures are exchanged between laboratories. Cultures are sent out to another investigator without being newly re-characterized, and even more frequently they are received and worked with by the recipient investigator who also fails to examine them for identity. Numerous reports during the past 25 years have documented instances of cross-contamination between cell cultures. Monitoring cell cultures for identity is a necessary but neglected adjunct control in the laboratory. Cells may be identified by isozymes, chromosomes, or species-specific antigens, and of course by information derived in combination from all three of these different cell markers. Each of the procedures yields rapid results and reasonable certainty of accuracy when properly controlled.

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