Application of an optical clearing agent during noninvasive laser coagulation of the canine vas deferens.

Development of a noninvasive vasectomy technique may eliminate male fear of complications and result in a more popular procedure. This study explores application of an optical clearing agent (OCA) to scrotal skin to reduce laser power necessary for successful noninvasive laser vasectomy and eliminate scrotal skin burns. A mixture of dimethyl sulfoxide and glycerol was noninvasively delivered into scrotal skin using a pneumatic jet device. Near-infrared laser radiation was delivered in conjunction with cryogen spray cooling to the skin surface in a canine model, ex vivo and in vivo. Burst pressure (BP) measurements were conducted to quantify strength of vas closure. A 30-min application of OCA improved skin transparency by 26+/-3%, reducing average power necessary for successful noninvasive laser vasectomy from 9.2 W without OCA (BP=291+/-31 mmHg) to 7.0 W with OCA (BP=292+/-19 mmHg). Control studies without OCA at 7.0 W failed to coagulate the vas with burst pressures (82+/-28 mmHg) significantly below typical ejaculation pressures (136+/-29 mmHg). Application of an OCA reduced the laser power necessary for successful noninvasive thermal coagulation of the vas by approximately 25%. This technique may result in use of a less expensive laser and eliminate the formation of scrotal skin burns during the procedure.

[1]  Nathaniel M Fried,et al.  Noninvasive laser vasectomy: Preliminary ex vivo tissue studies , 2009, Lasers in surgery and medicine.

[2]  Bernard Choi,et al.  Enhancement of optical skin clearing efficacy using a microneedle roller. , 2008, Journal of biomedical optics.

[3]  William D Mosher,et al.  Fertility, contraception, and fatherhood: data on men and women from cycle 6 (2002) of the 2002 National Survey of Family Growth. , 2006, Vital and health statistics. Series 23, Data from the National Survey of Family Growth.

[4]  Bernard Choi,et al.  Collagen solubility correlates with skin optical clearing. , 2006, Journal of biomedical optics.

[5]  Nathaniel M. Fried,et al.  Noninvasive laser coagulation of the canine vas deferens, in vivo , 2010, BiOS.

[6]  Bernard Choi,et al.  Revisiting optical clearing with dimethyl sulfoxide (DMSO) , 2009, Lasers in surgery and medicine.

[7]  Bernard Choi,et al.  Determination of chemical agent optical clearing potential using in vitro human skin , 2005, Lasers in surgery and medicine.

[8]  A J Welch,et al.  Using sandpaper for noninvasive transepidermal optical skin clearing agent delivery. , 2006, Journal of biomedical optics.

[9]  A J Welch,et al.  Use of osmotically active agents to alter optical properties of tissue: Effects on the detected fluorescence signal measured through skin , 2001, Lasers in surgery and medicine.

[10]  Jason Hsia,et al.  Vasectomy in the United States, 2002. , 2006, The Journal of urology.

[11]  V V Tuchin,et al.  Optical clearing of skin using flashlamp‐induced enhancement of epidermal permeability , 2006, Lasers in surgery and medicine.

[12]  Ilya Yaroslavsky,et al.  Lattice of optical islets: a novel treatment modality in photomedicine , 2005 .

[13]  William D Mosher,et al.  Use of contraception and use of family planning services in the United States: 1982-2002. , 2004, Advance data.

[14]  W. Miller,et al.  Tubal sterilization or vasectomy: how do married couples make the choice? , 1991, Fertility and sterility.

[15]  A. Shafik,et al.  Electrovasogram: a canine study of the electromechanical activity of the vas deferens. , 1995, Urology.

[16]  V. Tuchin,et al.  A Clear Vision for Laser Diagnostics (Review) , 2007, IEEE Journal of Selected Topics in Quantum Electronics.

[17]  William D Mosher,et al.  Fertility, family planning, and reproductive health of U.S. women: data from the 2002 National Survey of Family Growth. , 2005, Vital and health statistics. Series 23, Data from the National Survey of Family Growth.

[18]  Valery V Tuchin,et al.  Optical clearing of human skin: comparative study of permeability and dehydration of intact and photothermally perforated skin. , 2008, Journal of biomedical optics.

[19]  Xiangqun Xu,et al.  Synergistic effect of hyperosmotic agents of dimethyl sulfoxide and glycerol on optical clearing of gastric tissue studied with near infrared spectroscopy. , 2004, Physics in medicine and biology.

[20]  W. Miller,et al.  Factors associated with married women's selection of tubal sterilization and vasectomy. , 1985, Fertility and sterility.

[21]  M. D. Edmiston.  A liquid prism for refractive index studies , 2001 .

[22]  Bernard Choi,et al.  Following dimethyl sulfoxide skin optical clearing dynamics with quantitative nonlinear multimodal microscopy. , 2009, Applied optics.

[23]  Qingming Luo,et al.  Enhancement of skin optical clearing efficacy using photo‐irradiation , 2010, Lasers in surgery and medicine.

[24]  Massoud Motamedi,et al.  Temporary dermal scatter reduction: Quantitative assessment and implications for improved laser tattoo removal , 2005, Lasers in surgery and medicine.

[25]  Risto Myllylä,et al.  Detecting glucose-induced changes in in vitro and in vivo experiments with optical coherence tomography. , 2008, Journal of biomedical optics.

[26]  A J Welch,et al.  Enhancement of transepidermal skin clearing agent delivery using a 980 nm diode laser , 2005, Lasers in surgery and medicine.

[27]  Dan Zhu,et al.  Influence of alcohols on the optical clearing effect of skin in vitro. , 2008, Journal of biomedical optics.

[28]  Valery V. Tuchin,et al.  Skin optical clearing for improvement of laser tattoo removal , 2007, International Conference on Lasers, Applications, and Technologies.

[29]  H. G. Rylander,et al.  Use of an agent to reduce scattering in skin , 1999, Lasers in surgery and medicine.