Hydrogel Templates for Rapid Manufacturing of Bioactive Fibers and 3D Constructs

Hydrogel templates are formed to entrap various pre-polymers prior to their crosslinking process. Upon the completion of the crosslinking process, an independent polymer network with the same fiber geometry is formed. The hydrogel template can be removed if necessary. As the proof-of-principle, fibers from various polymers are fabricated. The fabricated hybrid polymeric fibers are bioactive and can be bioprinted or assembled using textile processes. The approach can be used for creating complex 3D constructs for various applications.

[1]  A. Tamayol,et al.  Effects of microstructure on flow properties of fibrous porous media at moderate Reynolds number. , 2012, Physical review. E, Statistical, nonlinear, and soft matter physics.

[2]  Jin-Hee Moon,et al.  Microfluidic Spinning of Flat Alginate Fibers with Grooves for Cell‐Aligning Scaffolds , 2012, Advanced materials.

[3]  A. Khademhosseini,et al.  Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. , 2014, Lab on a chip.

[4]  Samuel K Sia,et al.  Dynamic Hydrogels: Switching of 3D Microenvironments Using Two‐Component Naturally Derived Extracellular Matrices , 2010, Advanced materials.

[5]  Sang-Hoon Lee,et al.  Microfluidic spinning of micro- and nano-scale fibers for tissue engineering. , 2014, Lab on a chip.

[6]  A. Khademhosseini,et al.  Composite Living Fibers for Creating Tissue Constructs Using Textile Techniques , 2014, Advanced functional materials.

[7]  Shinji Sakai,et al.  Novel technique to control inner and outer diameter of calcium-alginate hydrogel hollow microfibers, and immobilization of mammalian cells , 2010 .

[8]  Ali Khademhosseini,et al.  Stop-flow lithography to generate cell-laden microgel particles. , 2008, Lab on a chip.

[9]  A. Khademhosseini,et al.  Microfluidic fabrication of microengineered hydrogels and their application in tissue engineering. , 2012, Lab on a chip.

[10]  Ali Khademhosseini,et al.  Microfluidic chip-based fabrication of PLGA microfiber scaffolds for tissue engineering. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[11]  S. Ostrovidov,et al.  Microfluidic Spinning of Cell‐Responsive Grooved Microfibers , 2015 .

[12]  Karen L. Smith,et al.  Biohybrid Carbon Nanotube/Agarose Fibers for Neural Tissue Engineering , 2011, Advanced functional materials.

[13]  Xiaoyi Wu,et al.  Wet-spinning of recombinant silk-elastin-like protein polymer fibers with high tensile strength and high deformability. , 2009, Biomacromolecules.

[14]  Shoji Takeuchi,et al.  Metre-long cell-laden microfibres exhibit tissue morphologies and functions. , 2013, Nature materials.

[15]  Seung-Joon Song,et al.  Sodium alginate hydrogel-based bioprinting using a novel multinozzle bioprinting system. , 2011, Artificial organs.

[16]  Marco Rasponi,et al.  Anisotropic material synthesis by capillary flow in a fluid stripe. , 2011, Biomaterials.

[17]  Seung‐Man Yang,et al.  High-throughput optofluidic platforms for mosaicked microfibers toward multiplex analysis of biomolecules. , 2012, Lab on a chip.

[18]  Federico Vozzi,et al.  Study of the crosstalk between hepatocytes and endothelial cells using a novel multicompartmental bioreactor: a comparison between connected cultures and cocultures. , 2009, Tissue engineering. Part A.

[19]  A. Khademhosseini,et al.  Cell-laden microengineered gelatin methacrylate hydrogels. , 2010, Biomaterials.

[20]  Duu-Jong Lee,et al.  Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis , 2012 .

[21]  S. Minko,et al.  Wet‐Spun Stimuli‐Responsive Composite Fibers with Tunable Electrical Conductivity , 2013 .

[22]  Hyeongjin Lee,et al.  Fabrication of cell-laden three-dimensional alginate-scaffolds with an aerosol cross-linking process , 2012 .

[23]  Ali Khademhosseini,et al.  Micro/Nanometer‐Scale Fiber with Highly Ordered Structures by Mimicking the Spinning Process of Silkworm , 2013, Advanced materials.

[24]  David Juncker,et al.  Microfluidic direct writer with integrated declogging mechanism for fabricating cell-laden hydrogel constructs , 2014, Biomedical microdevices.

[25]  Ali Khademhosseini,et al.  Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels , 2012, Advanced functional materials.

[26]  David J Mooney,et al.  Alginate hydrogels as biomaterials. , 2006, Macromolecular bioscience.

[27]  A. Khademhosseini,et al.  Highly Elastic Micropatterned Hydrogel for Engineering Functional Cardiac Tissue , 2013, Advanced functional materials.

[28]  Ali Khademhosseini,et al.  Digitally tunable physicochemical coding of material composition and topography in continuous microfibres. , 2011, Nature materials.

[29]  Yongping Chen,et al.  Bioinspired Multicompartmental Microfibers from Microfluidics , 2014, Advanced materials.

[30]  J. West,et al.  Rapid healing of femoral defects in rats with low dose sustained BMP2 expression from PEGDA hydrogel microspheres , 2013, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[31]  Chaenyung Cha,et al.  25th Anniversary Article: Rational Design and Applications of Hydrogels in Regenerative Medicine , 2014, Advanced materials.

[32]  Ali Khademhosseini,et al.  Fiber-based tissue engineering: Progress, challenges, and opportunities. , 2013, Biotechnology advances.