The Expression of CCAT2, UCA1, PANDA and GHET1 Long Non-coding RNAs in Lung Cancer.

Background Long non-coding RNAs (lncRNAs) have been considered to be prospective biomarkers for diagnosing lung cancer due to the fundamental roles they hold in the regulating several cancer-related pathways. Methods Using the quantitative real-time polymerase chain reaction method, we evaluated the expression of CCAT2, UCA1, PANDA and GHET1 lncRNAs in 32 lung cancer tissue samples and their corresponding adjacent non-cancerous tissues (ANCTs) from lung cancer patients admitted to the Labbafi-Nejad Hospital from 2015 to 2016. Results No significant differences were found in the expression of lncRNAs within the tumoral and non-tumoral tissue samples. Bayesian Multilevel analysis showed no association between the expression of lncRNAs and the patient's tumor node metastasis (TNM) stage following adjustments for age. Spearman correlation analysis revealed an inverse correlation between the expression of PANDA in tumoral tissues and age. Additionally, the difference in CCAT2 expression among the tumoral and non-tumoral tissues was inversely correlated with patients' age. Significant pairwise correlations were found between the expression of lncRNAs in both the tumoral and non-tumoral tissues. Conclusion Despite the findings supporting a role for the lncRNAs, CCAT2, UCA1, PANDA and GHET1 in the pathogenesis of lung cancer, our data suggests no relationship for expression of these lncRNAs in lung cancer, questioning their potential as lung cancer biomarkers.

[1]  F. Jacobs,et al.  Long Non-Coding RNAs in Neuronal Aging , 2018, Non-coding RNA.

[2]  J. de Vos,et al.  Differential long non-coding RNA expression profiles in human oocytes and cumulus cells , 2018, Scientific Reports.

[3]  Zhen-Biao Guan,et al.  Knockdown of lncRNA GHET1 suppresses cell proliferation, invasion and LATS1/YAP pathway in non small cell lung cancer. , 2017, Cancer biomarkers : section A of Disease markers.

[4]  Yu-jie Lei,et al.  Expression of PANDA, LincRNA-p21, PUMA in lung tissues of lung cancer patients in the Xuanwei and non-Xuanwei areas of Yunnan Province , 2017 .

[5]  Ju Wang,et al.  LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer. , 2017, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[6]  Bing Li,et al.  LncRNA-UCA1 exerts oncogenic functions in non-small cell lung cancer by targeting miR-193a-3p. , 2016, Cancer letters.

[7]  Jun Li,et al.  Emerging role of long noncoding RNAs in lung cancer: Current status and future prospects. , 2016, Respiratory medicine.

[8]  Yi Feng,et al.  Long Non-Coding RNAs , 2016, Methods in Molecular Biology.

[9]  Yi Liu,et al.  Posttranscriptional silencing of the lncRNA MALAT1 by miR-217 inhibits the epithelial-mesenchymal transition via enhancer of zeste homolog 2 in the malignant transformation of HBE cells induced by cigarette smoke extract. , 2015, Toxicology and applied pharmacology.

[10]  Huimin Wang,et al.  Upregulated lncRNA-UCA1 contributes to progression of lung cancer and is closely related to clinical diagnosis as a predictive biomarker in plasma. , 2015, International journal of clinical and experimental medicine.

[11]  R. Kong,et al.  Low expression of long noncoding RNA PANDAR predicts a poor prognosis of non-small cell lung cancer and affects cell apoptosis by regulating Bcl-2 , 2015, Cell Death and Disease.

[12]  Qizhan Liu,et al.  Epithelial-mesenchymal transition and cancer stem cells, mediated by a long non-coding RNA, HOTAIR, are involved in cell malignant transformation induced by cigarette smoke extract. , 2015, Toxicology and applied pharmacology.

[13]  I. Adcock,et al.  Microarray analysis of long non-coding RNAs in COPD lung tissue , 2014, Inflammation Research.

[14]  Rong Yin,et al.  CCAT2 is a lung adenocarcinoma-specific long non-coding RNA and promotes invasion of non-small cell lung cancer , 2014, Tumor Biology.

[15]  J. Martínez-Guitarte,et al.  Overexpression of long non-coding RNAs following exposure to xenobiotics in the aquatic midge Chironomus riparius. , 2012, Aquatic toxicology.