Self-Assembled Collagen Microparticles by Aerosol as a Versatile Platform for Injectable Anisotropic Materials.

Extracellular matrices (ECM) rich in type I collagen exhibit characteristic anisotropic ultrastructures. Nevertheless, working in vitro with this biomacromolecule remains challenging. When processed, denaturation of the collagen molecule is easily induced in vitro avoiding proper fibril self-assembly and further hierarchical order. Here, an innovative approach enables the production of highly concentrated injectable collagen microparticles, based on collagen molecules self-assembly, thanks to the use of spray-drying process. The versatility of the process is shown by performing encapsulation of secretion products of gingival mesenchymal stem cells (gMSCs), which are chosen as a bioactive therapeutic product for their potential efficiency in stimulating the regeneration of a damaged ECM. The injection of collagen microparticles in a cell culture medium results in a locally organized fibrillar matrix. The efficiency of this approach for making easily handleable collagen microparticles for encapsulation and injection opens perspectives in active tissue regeneration and 3D bioprinted scaffolds.

[1]  P. G. Campbell,et al.  3D bioprinting of collagen to rebuild components of the human heart , 2019, Science.

[2]  Y. Kuwahara,et al.  Mesenchymal Stem Cells on Horizon: A New Arsenal of Therapeutic Agents , 2018, Stem Cell Reviews and Reports.

[3]  Jun Kameoka,et al.  Pneumatically Actuated Soft Micromold Device for Fabricating Collagen and Matrigel Microparticles. , 2017, Soft robotics.

[4]  M. Raimondi,et al.  Secretome released from hydrogel-embedded adipose mesenchymal stem cells protects against the Parkinson’s disease related toxin 6-hydroxydopamine , 2017, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[5]  C. GettlerBrian,et al.  Formation of Adipose Stromal Vascular Fraction Cell-Laden Spheroids Using a Three-Dimensional Bioprinter and Superhydrophobic Surfaces , 2017 .

[6]  Cato T Laurencin,et al.  Microsphere-Based Scaffolds in Regenerative Engineering. , 2017, Annual review of biomedical engineering.

[7]  Seok Chung,et al.  Fabrication of type I collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell–ECM interactions , 2016, Biofabrication.

[8]  B. Haye,et al.  Involvement of 3D osteoblast migration and bone apatite during in vitro early osteocytogenesis. , 2016, Bone.

[9]  A. Miri,et al.  A gel aspiration-ejection system for the controlled production and delivery of injectable dense collagen scaffolds , 2016, Biofabrication.

[10]  E. Tartour,et al.  Inhibition of the Differentiation of Monocyte-Derived Dendritic Cells by Human Gingival Fibroblasts , 2013, PloS one.

[11]  A. Caplan,et al.  Mesenchymal Stem Cells in Tissue Repair , 2013, Front. Immunol..

[12]  N. Nassif,et al.  CHAPTER 5:Collagen‐based Materials for Tissue Repair, from Bio‐inspired to Biomimetic , 2013 .

[13]  Yan Wang,et al.  The predominant role of collagen in the nucleation, growth, structure and orientation of bone apatite. , 2012, Nature materials.

[14]  Yan Wang,et al.  Controlled collagen assembly to build dense tissue-like materials for tissue engineering , 2011 .

[15]  David Grosso,et al.  Aerosol Route to Functional Nanostructured Inorganic and Hybrid Porous Materials , 2011, Advanced materials.

[16]  B. Alonso,et al.  Chitin-silica nanocomposites by self-assembly. , 2010, Angewandte Chemie.

[17]  B. Coulomb,et al.  Multipotent progenitor cells in gingival connective tissue. , 2010, Tissue engineering. Part A.

[18]  A. Forbes,et al.  Microporous collagen spheres produced via thermally induced phase separation for tissue regeneration. , 2010, Acta biomaterialia.

[19]  S. Tsai,et al.  Influence of topography of nanofibrils of three-dimensional collagen gel beads on the phenotype, proliferation, and maturation of osteoblasts. , 2009, Journal of biomedical materials research. Part A.

[20]  G. Mosser,et al.  Liquid crystallinity in collagen systems in vitro and in vivo , 2008 .

[21]  P. Panine,et al.  Fibrillogenesis in dense collagen solutions: a physicochemical study. , 2008, Journal of molecular biology.

[22]  P. Panine,et al.  Cooperative ordering of collagen triple helices in the dense state. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[23]  C. Werner,et al.  Electrostatic interactions modulate the conformation of collagen I. , 2007, Biophysical journal.

[24]  Joachim Dissemond,et al.  Influence of pH on wound-healing: a new perspective for wound-therapy? , 2007, Archives of Dermatological Research.

[25]  David G Simpson,et al.  Electrospinning of collagen nanofibers. , 2002, Biomacromolecules.

[26]  S. Chueh,et al.  Microspheres of hydroxyapatite/reconstituted collagen as supports for osteoblast cell growth. , 1999, Biomaterials.

[27]  S. Davis,et al.  Chitosan microspheres prepared by spray drying. , 1999, International journal of pharmaceutics.

[28]  W. Friess,et al.  Collagen--biomaterial for drug delivery. , 1998, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[29]  M. Giraud‐Guille,et al.  Stabilization of fluid cholesteric phases of collagen to ordered gelated matrices. , 1995, Journal of molecular biology.

[30]  C. A. Miles,et al.  The kinetics of the thermal denaturation of collagen in unrestrained rat tail tendon determined by differential scanning calorimetry. , 1995, Journal of molecular biology.

[31]  Toshio Miyata,et al.  Wind-induced Flutter of Structures , 1986 .

[32]  M. Pineri,et al.  Water–collagen interactions: Calorimetric and mechanical experiments , 1978, Biopolymers.

[33]  S. Krimm,et al.  Effwct of temperature on the circular dichroism spectra of polypeptides in the extended state , 1972, Biopolymers.

[34]  Song Li,et al.  Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra , 2018 .

[35]  Richard Weinkamer,et al.  Materials design inspired by nature : function through inner architecture , 2013 .

[36]  M. Giraud‐Guille,et al.  Banded patterns in liquid crystalline phases of type I collagen: relationship with crimp morphology in connective tissue architecture. , 1998, Connective tissue research.

[37]  K. Leong,et al.  Microcapsules obtained from complex coacervation of collagen and chondroitin sulfate. , 1995, Journal of biomaterials science. Polymer edition.