Flower-like gold nanoparticles-based immunochromatographic test strip for rapid simultaneous detection of fumonisin B1 and deoxynivalenol in Chinese traditional medicine.

A flower-like gold nanoparticles (FGN)-based immunochromatographic test strip (ICS) was developed and used for the first time for the rapid simultaneous detection of fumonisin B1 (FB1) and deoxynivalenol (DON) in Chinese traditional medicine. Several experimental conditions affecting the sensitivity of ICS have been investigated, including the type of FGN, the preparation conditions of FGN-monoclonal antibody (MAb) conjugates, and the process parameters of ICS. Under the optimal experimental conditions, the visual limit of detection was 5.0 ng/mL (corresponding to 50 μg/kg in Chinese traditional medicine samples) for both FB1 and DON, and detection can be completed within 5 min. In addition, the natural samples were analyzed using high-performance liquid chromatography (HPLC) or enzyme-linked immunosorbent assay (ELISA), and the results of these methods showed good correlation with those obtained using ICS. The procedure using FGN-based simultaneous ICS was sensitive, rapid, and convenient for on-site detection of a large number of samples.

[1]  G. Frens.  Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions , 1973 .

[2]  Caiyun Jiang,et al.  A reproducible SERS substrate based on electrostatically assisted APTES-functionalized surface-assembly of gold nanostars. , 2011, ACS applied materials & interfaces.

[3]  Shijia Yang,et al.  Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol. , 2014, Analytica chimica acta.

[4]  Songcheng Yu,et al.  A lateral flow assay for simultaneous detection of Deoxynivalenol, Fumonisin B1 and Aflatoxin B1 , 2018, Toxicon : official journal of the International Society on Toxinology.

[5]  Shijia Yang,et al.  Chemiluminescence reaction kinetics-resolved multianalyte immunoassay strategy using a bispecific monoclonal antibody as the unique recognition reagent. , 2015, Analytical chemistry.

[6]  Shufang Wang,et al.  Simultaneous determination of seventeen mycotoxins residues in Puerariae lobatae radix by liquid chromatography-tandem mass spectrometry. , 2014, Journal of pharmaceutical and biomedical analysis.

[7]  Yang Xu,et al.  Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains , 2015, Analytical and Bioanalytical Chemistry.

[8]  Dong-ling Wu,et al.  Rapid Fabrication of Gold Nanoflowers Tuned by pH: Insights Into the Growth Mechanism. , 2015, Journal of nanoscience and nanotechnology.

[9]  Bashir Ahmad,et al.  Natural occurrence of mycotoxins in medicinal plants: a review. , 2014, Fungal genetics and biology : FG & B.

[10]  Aldo Roda,et al.  A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour. , 2015, The Analyst.

[11]  B. Hammock,et al.  Anti-idiotypic nanobody-alkaline phosphatase fusion proteins: Development of a one-step competitive enzyme immunoassay for fumonisin B1 detection in cereal. , 2016, Analytica chimica acta.

[12]  C. Frizzell,et al.  An in vitro investigation on the cytotoxic and nuclear receptor transcriptional activity of the mycotoxins fumonisin B1 and beauvericin. , 2016, Toxicology letters.

[13]  A. Roda,et al.  Chemiluminescence-based biosensor for fumonisins quantitative detection in maize samples. , 2012, Biosensors & bioelectronics.

[14]  Yonghua Xiong,et al.  Detection of aflatoxin B₁ with immunochromatographic test strips: Enhanced signal sensitivity using gold nanoflowers. , 2015, Talanta.

[15]  Hong Liu,et al.  Controllable synthesis of three-dimensional branched gold nanocrystals assisted by cationic surfactant poly(diallyldimethylammonium) chloride in acidic aqueous solution , 2014 .

[16]  C. Baggiani,et al.  A lateral flow immunoassay for straightforward determination of fumonisin mycotoxins based on the quenching of the fluorescence of CdSe/ZnS quantum dots by gold and silver nanoparticles , 2018, Microchimica Acta.

[17]  H. Ouyang,et al.  Colorimetric/chemiluminescent immunochromatographic test strip by using luminol-reduced gold nanoparticles as dual-response probes , 2018, Sensors and Actuators B: Chemical.

[18]  S. Alzamora,et al.  Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method , 2016 .

[19]  Eugenio Alladio,et al.  Colour-encoded lateral flow immunoassay for the simultaneous detection of aflatoxin B1 and type-B fumonisins in a single Test line. , 2019, Talanta.

[20]  A. Zherdev,et al.  Gold nanoparticles of different shape for bicolor lateral flow test. , 2019, Analytical biochemistry.

[21]  Suxia Zhang,et al.  Gold nanoparticles-based lateral flow immunoassay with silver staining for simultaneous detection of fumonisin B1 and deoxynivalenol , 2015 .

[22]  Zhenhong Zhuang,et al.  Rapid detection of fumonisin B1 using a colloidal gold immunoassay strip test in corn samples. , 2015, Toxicon : official journal of the International Society on Toxinology.

[23]  Yongning Wu,et al.  Risk assessment of deoxynivalenol in high-risk area of China by human biomonitoring using an improved high throughput UPLC-MS/MS method , 2018, Scientific Reports.

[24]  Liegang Liu,et al.  Deoxynivalenol, gut microbiota and immunotoxicity: A potential approach? , 2018, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[25]  O. Busto,et al.  Determination of some flavan-3-ols and anthocyanins in red grape seed and skin extracts by HPLC-DAD: Validation study and response comparison of different standards , 2008 .

[26]  Qinghua He,et al.  A peptide/maltose-binding protein fusion protein used to replace the traditional antigen for immunological detection of deoxynivalenol in food and feed. , 2018, Food chemistry.

[27]  Laura Anfossi,et al.  Development and application of a quantitative lateral flow immunoassay for fumonisins in maize. , 2010, Analytica chimica acta.

[28]  Rudolf Krska,et al.  Quantitation of mycotoxins in food and feed from Burkina Faso and Mozambique using a modern LC-MS/MS multitoxin method. , 2012, Journal of agricultural and food chemistry.

[29]  C. Baggiani,et al.  Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins , 2017, Microchimica Acta.