Effect of cycling temperatures on the production of deoxynivalenol and zearalenone by Fusarium graminearum NRRL 5883.

The effects of three regimens of cycling incubation temperatures and incubation at constant 25 degrees C on the growth of Fusarium graminearum NRRL 5883 and production of deoxynivalenol (DON) and zearalenone (ZEN) on rice were compared. The effects of low-temperature stress were also studied by incubating rice cultures at a constant 15 degrees C for 4 weeks following incubation at constant 25 degrees C for 2 weeks. Both incubation temperature and time significantly (P < or = 0.05) affected growth of F. graminearum NRRL 5883 and production of DON and ZEN. The highest amount of free ergosterol (640 microg/g culture material) that was used as a measure of fungal growth was found in cultures incubated at temperatures cycling between 15 and 30 degrees C during a 6-week period. The highest amounts of DON (1,679 microg/g culture material) and ZEN (603 microg/g culture material) were produced in cultures incubated at a constant 25 degrees C for 2 weeks prior to incubation at a constant 15 degrees C for an additional 4 weeks. Under cycling incubation temperatures, maximum amounts of DON (850 microg/g culture material) and ZEN (98 microg/g culture material) were produced in cultures incubated at temperatures cycling between 15 and 30 degrees C for 6 weeks. Overall, there was no correlation between mold growth and production of either DON or ZEN. However, DON production and ZEN production were correlated.

[1]  H. Trenholm,et al.  Analysis of Trichothecene Mycotoxins by Gas Chromatography with Electron-Capture Detection , 1994 .

[2]  D. H. Lee,et al.  Natural occurrence of Fusarium mycotoxins (trichothecenes and zearalenone) in barley and corn in Korea , 1993, Applied and environmental microbiology.

[3]  P. Nelson,et al.  Analysis of naturally occurring mycotoxins in feedstuffs and food. , 1993, Journal of animal science.

[4]  A. P. Damoglou,et al.  Toxin production by Fusarium culmorum IMI 309344 and F. graminearum NRRL 5883 on grain substrates , 1993 .

[5]  S. Resnik,et al.  Influence of water activity and temperature on the accumulation of zearalenone in corn. , 1988, International journal of food microbiology.

[6]  B. K. Thompson,et al.  Chemical, nutritive, deoxynivalenol and zearalenone content of corn relative to the site of inoculation with different isolates of Fusarium graminearum , 1988 .

[7]  J. Pestka,et al.  Emetic activity of the trichothecene 15-acetyldeoxynivalenol in swine. , 1987, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[8]  S. Sundlof,et al.  Zearalenone and zeranol: potential residue problems in livestock. , 1986, Veterinary and human toxicology.

[9]  J. J. Ellis,et al.  Production of deoxynivalenol and zearalenone by isolates of Fusarium graminearum Schw , 1986, Journal of basic microbiology.

[10]  W. Kwolek,et al.  Liquid chromatographic determination of alpha-zearalenol and zearalenone in corn: collaborative study. , 1985, Journal - Association of Official Analytical Chemists.

[11]  H. Gelberg,et al.  Preliminary study of the pharmacokinetics and toxicopathy of deoxynivalenol (vomitoxin) in swine. , 1985, American journal of veterinary research.

[12]  Kun-Young Park,et al.  Effect of Cycling Temperatures on Aflatoxin Production by Aspergillus parasiticus and Aspergillus flavus in Rice and Cheddar Cheese , 1983 .

[13]  P. E. Nelson,et al.  Fusarium species: an illustrated manual for identification. , 1983 .

[14]  Kun-Young Park,et al.  Increased Aflatoxin Production by Aspergillus parasiticus Under Conditions of Cycling Temperatures , 1981 .

[15]  P. Koehler,et al.  Effect of Temperature Cycling on the Production of Patulin and Citrinin , 1981 .

[16]  P. Koehler,et al.  Influence of temperature cycling on the production of aflatoxins B1 and G1 by Aspergillus parasiticus , 1980, Applied and environmental microbiology.

[17]  H. Funnell,et al.  RELATION OF WEATHER VARIABLES TO INCIDENCE OF ZEARALENONE IN CORN IN SOUTHERN ONTARIO , 1980 .

[18]  G. Barron,et al.  INFLUENCE OF TEMPERATURE ON THE STRAIN OF FUSARIUM GRAMINEARUM SCHWABE IN ZEARALENONE PRODUCTION , 1978 .

[19]  J. Tuite,et al.  Emetic and refusal activity of deoxynivalenol to swine , 1977, Applied and environmental microbiology.

[20]  C. Christensen,et al.  Factors affecting production of the mycotoxin F-2 by Fusarium roseum. , 1970, Phytopathology.

[21]  C. Christensen,et al.  Estrogenic metabolite produced by Fusarium graminearum in stored corn. , 1967, Applied microbiology.

[22]  J. J. Ellis,et al.  Production of deoxynivalenol and zearalenone by isolates of } ! , usarium graminearum , 2022 .