Determination of membrane thickness distribution from orthogonal intercepts

This report deals with the reconstruction of the distribution of membrane thickness T from that of orthogonal intercept length L0, measured in random section planes. In such planes the membrane appears as a band and the linear distance from one of its boundaries perpendicular to the opposite one is the length of the orthogonal intercept. Using a membrane model, an integral equation relating the probability density functions of orthogonal intercept length f(l0) and membrane thickness g(τ) is derived. Relations between moments are derived and the analytic solution to the problem of reconstructing g(τ) from f(l0) is given. The parametric approach by which it is assumed that g(τ) has some known analytic form with unknown parameters is considered, and the use of a suggested analytic form for describing the thickness distribution of the human glomerular basement membrane is discussed.

[1]  R. Østerby,et al.  Distribution of Membrane Thickness Determined By Lineal Analysis , 1978, Journal of microscopy.

[2]  H J Gundersen,et al.  Estimators of the number of objects per area unbiased by edge effects. , 1978, Microscopica acta.

[3]  P. Moran THE PROBABALISTIC BASIS OF STEREOLOGY , 1972 .

[4]  R. Østerby,et al.  Statistical analysis of transformations leading to normal distribution of measurements of the peripheral glomerular basement membrane , 1973, Journal of microscopy.

[5]  R. Osterby Quantitative electron microscopy of the glomerular basement membrane. A methodologic study. , 1971, Laboratory investigation; a journal of technical methods and pathology.

[6]  H. Riedwyl,et al.  Bestimmung der Größenverteilung von Kugeln aus Schnittkreisradien , 1970 .

[7]  E. Stacy A Generalization of the Gamma Distribution , 1962 .

[8]  Robert S. Anderssen,et al.  Abel type integral equations in stereology. II. Computational methods of solution and the random spheres approximation , 1975 .

[9]  N. Suwa,et al.  Morphometrical method to estimate the parameters of distribution functions assumed for spherical bodies from measurements on a random section. , 1976, The Tohoku journal of experimental medicine.

[10]  R. Unger,et al.  Studies of muscle capillary basement membranes in normal subjects, diabetic, and prediabetic patients. , 1968, The Journal of clinical investigation.

[11]  Bruce W. Knight,et al.  A MORPHOMETRIC STUDY ON THE THICKNESS OF THE PULMONARY AIR-BLOOD BARRIER , 1964, The Journal of cell biology.

[12]  R. Østerby,et al.  Sampling Problems in the Kidney , 1978 .

[13]  N Keiding,et al.  Maximum likelihood estimation of the size distribution of liver cell nuclei from the observed distribution in a plane section. , 1972, Biometrics.

[14]  C. Kilo,et al.  Muscle Capillary Basement Membrane Changes Related to Aging and to Diabetes Mellitus , 1972, Diabetes.