Current strategies for cell delivery in cartilage and bone regeneration.

Several cell-based tissue-engineering therapies are emerging to regenerate damaged tissues. These strategies use autologous cells in combination with bioresorbable delivery materials. Major functions of a delivery scaffold are to provide initial mechanical stability, homogenous three-dimensional cell distribution, improved tissue differentiation, suitable handling and properties for delivery and fixation into patients. Delivery of cells can be achieved using injectable matrices, soft scaffolds, membranes, solid load-bearing scaffolds or immunoprotective macroencapsulation. Thus, to expand the clinical potential, next generation therapies will depend on smart delivery concepts that make use of the regenerative potential of stem cells, morphogenetic growth factors and biomimetic materials.

[1]  K. Shakesheff,et al.  Novel osteoinductive biomimetic scaffolds stimulate human osteoprogenitor activity--implications for skeletal repair. , 2003, Connective tissue research.

[2]  D. Eyre,et al.  Assembly of collagen types II, IX and XI into nascent hetero-fibrils by a rat chondrocyte cell line. , 2003, European journal of biochemistry.

[3]  Farshid Guilak,et al.  Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds. , 2004, Biomaterials.

[4]  Eben Alsberg,et al.  SHAPE-DEFINING SCAFFOLDS FOR MINIMALLY INVASIVE TISSUE ENGINEERING , 2004, Transplantation.

[5]  L Sedel,et al.  A biodegradable fibrin scaffold for mesenchymal stem cell transplantation. , 2003, Biomaterials.

[6]  David L. Cochran,et al.  Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies. , 2003, European cells & materials.

[7]  C. Ohlsson,et al.  Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. , 1994, The New England journal of medicine.

[8]  Ying Luo,et al.  A photolabile hydrogel for guided three-dimensional cell growth and migration , 2004, Nature materials.

[9]  R. Langer,et al.  Biodegradable, Elastic Shape-Memory Polymers for Potential Biomedical Applications , 2002, Science.

[10]  R. Schmelzeisen,et al.  Tissue-engineered bone for maxillary sinus augmentation. , 2004, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[11]  R. Eavey,et al.  Tissue Engineering of a Human Sized And Shaped Auricle Using a Mold , 2004, The Laryngoscope.

[12]  C. Perka,et al.  Matrix engineering for osteogenic differentiation of rabbit periosteal cells using alpha-tricalcium phosphate particles in a three-dimensional fibrin culture. , 2002, Journal of biomedical materials research.

[13]  A. Mikos,et al.  Injectable biodegradable polymer composites based on poly(propylene fumarate) crosslinked with poly(ethylene glycol)-dimethacrylate. , 2000, Biomaterials.

[14]  D. Hutmacher,et al.  Osteogenic induction of human bone marrow-derived mesenchymal progenitor cells in novel synthetic polymer-hydrogel matrices. , 2003, Tissue engineering.

[15]  Kristi S Anseth,et al.  In vitro osteogenic differentiation of human mesenchymal stem cells photoencapsulated in PEG hydrogels. , 2004, Journal of biomedical materials research. Part A.

[16]  R. Schmelzeisen,et al.  Making bone: implant insertion into tissue-engineered bone for maxillary sinus floor augmentation-a preliminary report. , 2003, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery.

[17]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[18]  A. Mikos,et al.  Synthesis of in situ cross-linkable macroporous biodegradable poly(propylene fumarate-co-ethylene glycol) hydrogels. , 2002, Biomacromolecules.

[19]  Makarand V Risbud,et al.  Tissue engineering: advances in in vitro cartilage generation. , 2002, Trends in biotechnology.

[20]  K. Burg,et al.  Characterization of cellular carriers for use in injectable tissue-engineering composites. , 2003, Journal of biomedical materials research. Part A.

[21]  A. Mikos,et al.  Modulation of differentiation and mineralization of marrow stromal cells cultured on biomimetic hydrogels modified with Arg-Gly-Asp containing peptides. , 2004, Journal of biomedical materials research. Part A.

[22]  J. Leroux,et al.  Novel injectable neutral solutions of chitosan form biodegradable gels in situ. , 2000, Biomaterials.

[23]  W W Minuth,et al.  Tissue engineering and autologous transplant formation: practical approaches with resorbable biomaterials and new cell culture techniques. , 1996, Biomaterials.

[24]  T. Albert,et al.  Stem cell regeneration of the nucleus pulposus. , 2004, The spine journal : official journal of the North American Spine Society.

[25]  M. Endres,et al.  Immunomodulation of tissue-engineered transplants: in vivo bone generation from methylprednisolone-stimulated chondrocytes , 2004, European Archives of Oto-Rhino-Laryngology and Head & Neck.

[26]  M. Sittinger,et al.  Macroencapsulation of human cartilage implants: pilot study with polyelectrolyte complex membrane encapsulation. , 2000, Biomaterials.

[27]  M Sittinger,et al.  Tissue engineering of biphasic joint cartilage transplants. , 1999, Biomaterials.

[28]  M. Risbud,et al.  Tissue engineering: Implications in the treatment of organ and tissue defects , 2004, Biogerontology.

[29]  Ralph Müller,et al.  Synthetic extracellular matrices for in situ tissue engineering , 2004, Biotechnology and bioengineering.

[30]  Michael Sittinger,et al.  The arthroscopic implantation of autologous chondrocytes for the treatment of full-thickness cartilage defects of the knee joint. , 2003, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[31]  R P Jakob,et al.  Articular cartilage repair using a tissue-engineered cartilage-like implant: an animal study. , 2001, Osteoarthritis and cartilage.

[32]  Alan Grodzinsky,et al.  Tissue-engineered composites for the repair of large osteochondral defects. , 2002, Arthritis and rheumatism.

[33]  David J Mooney,et al.  Protein-based signaling systems in tissue engineering. , 2003, Current opinion in biotechnology.

[34]  R J Composto,et al.  RGD Peptides Immobilized on a Mechanically Deformable Surface Promote Osteoblast Differentiation , 2002, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[35]  Arnold I Caplan,et al.  Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable calcium phosphate and hyaluronan sponge. , 2002, Tissue engineering.

[36]  F. Grassi,et al.  Arthroscopic autologous chondrocyte implantation for the treatment of a chondral defect in the tibial plateau of the knee. , 2004, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[37]  C. T. Vangsness,et al.  Restoring articular cartilage in the knee. , 2004, American journal of orthopedics.

[38]  K. Furukawa,et al.  Three-Dimensional Seeding of Chondrocytes Encapsulated in Collagen Gel into PLLA Scaffolds , 2002, Cell transplantation.

[39]  S. Teoh,et al.  Scaffold design and in vitro study of osteochondral coculture in a three-dimensional porous polycaprolactone scaffold fabricated by fused deposition modeling. , 2003, Tissue engineering.

[40]  Charles A. Vacanti,et al.  Transplantation of Chondrocytes Utilizing a Polymer‐Cell Construct to Produce Tissue‐Engineered Cartilage in the Shape of a Human Ear , 1997, Plastic and reconstructive surgery.

[41]  K. Gross,et al.  Biodegradable composite scaffolds with an interconnected spherical network for bone tissue engineering. , 2004, Biomaterials.

[42]  Dietmar W Hutmacher,et al.  Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. , 2004, Trends in biotechnology.

[43]  A. Reddi,et al.  Morphogenesis and tissue engineering of bone and cartilage: inductive signals, stem cells, and biomimetic biomaterials. , 2000, Tissue engineering.