Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

[1]  Channabasavaiah B. Gurumurthy,et al.  Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes , 2017, Methods.

[2]  David B. West,et al.  Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein , 2016, Transgenic Research.

[3]  Jon Cohen 'Any idiot can do it.' Genome editor CRISPR could put mutant mice in everyone's reach , 2016 .

[4]  Takashi Yamamoto,et al.  Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ , 2016, BMC Genomics.

[5]  B. Ellenbroek,et al.  Rodent models in neuroscience research: is it a rat race? , 2016, Disease Models & Mechanisms.

[6]  T. Aitman,et al.  A RATional choice for translational research? , 2016, Disease Models & Mechanisms.

[7]  Jeremy M. Stark,et al.  Regulation of Single-Strand Annealing and its Role in Genome Maintenance. , 2016, Trends in genetics : TIG.

[8]  Eric Smalley CRISPR mouse model boom, rat model renaissance , 2016, Nature Biotechnology.

[9]  M. Ikawa,et al.  CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice , 2016, Scientific Reports.

[10]  James E Haber,et al.  The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair. , 2016, DNA repair.

[11]  X. Liu,et al.  CRISPR: a versatile tool for both forward and reverse genetics research , 2016, Human Genetics.

[12]  Ryohei Yasuda,et al.  High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing , 2016, Cell.

[13]  C. Lowenstein,et al.  A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research. , 2016, Arteriosclerosis, thrombosis, and vascular biology.

[14]  L. Liaw,et al.  CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells , 2016, G3: Genes, Genomes, Genetics.

[15]  Lin He,et al.  Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes* , 2016, The Journal of Biological Chemistry.

[16]  M. Ohtsuka,et al.  Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new , 2016, Biology Direct.

[17]  A. Bajić,et al.  Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases , 2016, Scientific Reports.

[18]  H. Kiyonari,et al.  A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes , 2016, Genesis.

[19]  T. Takumi,et al.  Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system , 2016, Scientific Reports.

[20]  T. Mashimo,et al.  ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes , 2016, Nature Communications.

[21]  M. Ohtsuka,et al.  GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos , 2016, Current protocols in human genetics.

[22]  T. Horii,et al.  Challenges to increasing targeting efficiency in genome engineering , 2015, The Journal of reproduction and development.

[23]  Tetsushi Sakuma,et al.  MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems , 2015, Nature Protocols.

[24]  D. Fontaine,et al.  Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium , 2015, Diabetes.

[25]  S. Robertson,et al.  Expanding the power of recombinase-based labeling to uncover cellular diversity , 2015, Development.

[26]  L. Symington,et al.  Microhomology-Mediated End Joining: A Back-up Survival Mechanism or Dedicated Pathway? , 2015, Trends in biochemical sciences.

[27]  R. Lockey,et al.  Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes. , 2015, BioTechniques.

[28]  Masahiro Sato,et al.  CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA , 2015, Scientific Reports.

[29]  Clifford A. Meyer,et al.  Sequence determinants of improved CRISPR sgRNA design , 2015, Genome research.

[30]  M. Bühler,et al.  Single-Step Generation of Conditional Knockout Mouse Embryonic Stem Cells. , 2015, Cell reports.

[31]  M. Ohtsuka,et al.  GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice , 2015, Scientific Reports.

[32]  Takashi Yamamoto,et al.  Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice , 2015, Genome Biology.

[33]  Hidde L Ploegh,et al.  Inhibition of non-homologous end joining increases the efficiency of CRISPR/Cas9-mediated precise [TM: inserted] genome editing , 2015, Nature Biotechnology.

[34]  Tetsushi Sakuma,et al.  Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9 , 2014, Nature Communications.

[35]  Takashi Yamamoto,et al.  Simple knockout by electroporation of engineered endonucleases into intact rat embryos , 2014, Scientific Reports.

[36]  Donald W. Harms,et al.  Mouse Genome Editing Using the CRISPR/Cas System , 2014, Current protocols in human genetics.

[37]  Meagan E. Sullender,et al.  Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation , 2014, Nature Biotechnology.

[38]  Lianfeng Zhang,et al.  Generation of eGFP and Cre knockin rats by CRISPR/Cas9 , 2014, The FEBS journal.

[39]  K. C. K. Lloyd,et al.  Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice , 2014, FEBS open bio.

[40]  S. Takada,et al.  Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system , 2014, Scientific Reports.

[41]  Lianfeng Zhang,et al.  Generating Rats with Conditional Alleles Using Crispr/cas9 Dear Editor, Generating Conditional Knockout Rats Using Crispr/cas9 2 Generating Conditional Knockout Rats Using Crispr/cas9 4 , 2022 .

[42]  Feng Zhang,et al.  Genome engineering using CRISPR-Cas9 system. , 2015, Methods in molecular biology.

[43]  R. Jaenisch,et al.  One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering , 2013, Cell.

[44]  James E. DiCarlo,et al.  RNA-Guided Human Genome Engineering via Cas9 , 2013, Science.

[45]  Le Cong,et al.  Multiplex Genome Engineering Using CRISPR/Cas Systems , 2013, Science.

[46]  K. Lloyd A knockout mouse resource for the biomedical research community , 2011, Annals of the New York Academy of Sciences.

[47]  J. Harrow,et al.  A conditional knockout resource for the genome-wide study of mouse gene function , 2011, Nature.

[48]  W. O'Neill,et al.  Age-Related Hearing Loss in C57BL/6J Mice has both Frequency-Specific and Non-Frequency-Specific Components that Produce a Hyperacusis-Like Exaggeration of the Acoustic Startle Reflex , 2007, Journal of the Association for Research in Otolaryngology.

[49]  Wolfgang Wurst,et al.  A Mouse for All Reasons , 2007, Cell.

[50]  J. Whitsett,et al.  Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction , 2005, Nucleic Acids Research.

[51]  J. Whitsett,et al.  Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction , 2005, Nucleic acids research.

[52]  W. M. Weaver,et al.  A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival. , 2001, Immunity.

[53]  K. Rajewsky,et al.  Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. , 1994, Science.

[54]  A. D’Andrea,et al.  Repair Pathway Choices and Consequences at the Double-Strand Break. , 2016, Trends in cell biology.

[55]  K. C. K. Lloyd,et al.  CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies , 2016 .

[56]  T. Fielder,et al.  Transgenic Production Benchmarks , 2011 .

[57]  Thomas Helleday,et al.  Conservative homologous recombination preferentially repairs DNA double-strand breaks in the S phase of the cell cycle in human cells. , 2004, Nucleic acids research.

[58]  J. Lindeberg,et al.  Conditional gene targeting. , 2003, Upsala journal of medical sciences.