Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy

This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The oncentrations of progesterone (P4), oestrogen, growth hormone (GH), insulin-like growth factor-1 and -2 (IGF1, -2) and IGF-binding protein-2, -3 and -4 (IGFBP2, -3, -4) were measured. The mRNA expressions of growth hormone receptor 1A, IGF1, IGF2, IGFBP2, IGFBP3 and IGFBP4 were detected using RT-qPCR in liver biopsy specimens (day 18). In all groups, total serum IGF1 decreased from day 0 to 16. Notably, IGFBP4 maternal blood concentrations were lower during pregnancy than during non-pregnant cycles and synchronized control cycles. It can be speculated that the lower IGFBP4 in maternal blood may result in an increase of free IGF1 for local action. Further studies regarding IGFBP4 concentration and healthy early pregnancy are warranted.

[1]  I. Contreras-Solis,et al.  Periconceptional undernutrition modifies endocrine profiles and hepatic gene expression in sheep. , 2015, Journal of animal physiology and animal nutrition.

[2]  C. Wrenzycki,et al.  The somatotropic axis during the physiological estrus cycle in dairy heifers--Effect on hepatic expression of GHR and SOCS2. , 2015, Journal of dairy science.

[3]  M. Heppelmann,et al.  Antepartal insulin-like growth factor concentrations indicating differences in the metabolic adaptive capacity of dairy cows , 2014, Journal of veterinary science.

[4]  F. Metzger,et al.  Effects of parturition and feed restriction on concentrations and distribution of the insulin-like growth factor-binding proteins in plasma and cerebrospinal fluid of dairy cows. , 2014, Journal of dairy science.

[5]  S. Degrelle,et al.  Energy and lipid metabolism gene expression of D18 embryos in dairy cows is related to dam physiological status. , 2014, Physiological genomics.

[6]  H. Lashen,et al.  Fetal growth in relation to maternal and fetal IGF‐axes: a systematic review and meta‐analysis , 2013, Acta obstetricia et gynecologica Scandinavica.

[7]  F. Metzger,et al.  Hepatic mRNA expression of acid labile subunit and deiodinase 1 differs between cows selected for high versus low concentrations of insulin-like growth factor 1 in late pregnancy. , 2013, Journal of dairy science.

[8]  L. Rejnmark,et al.  Changes in calcitropic hormones, bone markers and insulin-like growth factor I (IGF-I) during pregnancy and postpartum: a controlled cohort study , 2013, Osteoporosis International.

[9]  M. Rodger,et al.  Significance of IGFBP-4 in the development of fetal growth restriction. , 2012, The Journal of clinical endocrinology and metabolism.

[10]  Agcatggcctgtacaacctc Gatcagcttcccggtgttag,et al.  Temporal changes in endometrial gene expression and protein localization of members of the IGF family in cattle : effects of progesterone and pregnancy , 2012 .

[11]  R. Bruckmaier,et al.  Oestradiol enhances plasma growth hormone and insulin-like growth factor-I concentrations and increased the expression of their receptors mRNAs in the liver of ovariectomized cows. , 2011, Reproduction in domestic animals = Zuchthygiene.

[12]  D. Schams,et al.  Changes in plasma metabolic hormone concentrations during the ovarian cycles of Japanese black and holstein cattle. , 2007, The Journal of reproduction and development.

[13]  J. Ross,et al.  Endocrine disruption of uterine insulin-like growth factor expression in the pregnant gilt. , 2005, Reproduction.

[14]  J. Féher,et al.  The insulin-like growth factor system: IGFs, IGF-binding proteins and IGFBP-proteases. , 2005, Acta physiologica Hungarica.

[15]  L. Spicer,et al.  Pregnancy-associated plasma protein-A and insulin-like growth factor binding protein mRNAs in granulosa cells of dominant and subordinate follicles of preovulatory cattle. , 2005, Domestic animal endocrinology.

[16]  S. Mohan,et al.  Up date on IGFBP-4: regulation of IGFBP-4 levels and functions, in vitro and in vivo. , 2004, Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society.

[17]  D. Portetelle,et al.  Role of the somatotropic axis in the mammalian metabolism. , 2002, Domestic animal endocrinology.

[18]  G. Mann,et al.  The expression of the IGF system in the bovine uterus throughout the oestrous cycle and early pregnancy. , 2000, The Journal of endocrinology.

[19]  S. Bell,et al.  Expression of mRNA encoding insulin‐like growth factors I and II by uterine tissues and placenta during pregnancy in the rat , 1999, Molecular reproduction and development.

[20]  G. Seidel,et al.  Characterization of insulin-like growth factor-binding proteins in the uterus and conceptus during early conceptus elongation in cattle. , 1998, Biology of reproduction.

[21]  L. Phillips,et al.  Molecular regulation of insulin-like growth factor-I and its principal binding protein, IGFBP-3. , 1998, Progress in nucleic acid research and molecular biology.

[22]  M. Iwashita,et al.  Profiles of insulin-like growth factor binding proteins and the protease activity in the maternal circulation and its local regulation between placenta and decidua. , 1997, Endocrine journal.

[23]  T. Braulke,et al.  Proteolysis of insulin-like growth factors (IGF) and IGF binding proteins by cathepsin D. , 1997, Endocrinology.

[24]  F. Bazer,et al.  Expression of messenger RNAs encoding insulin-like growth factor-I, -II, and insulin-like growth factor binding protein-2 in bovine endometrium during the estrous cycle and early pregnancy. , 1991, Biology of reproduction.

[25]  P. Bischof,et al.  The presence of pregnancy-associated plasma protein-A in human corpora lutea: cellular and subcellular distribution and dependence on reproductive state. , 1991, Biology of reproduction.

[26]  L. Giudice,et al.  Insulin-like growth factor binding proteins in maternal serum throughout gestation and in the puerperium: effects of a pregnancy-associated serum protease activity. , 1990, The Journal of clinical endocrinology and metabolism.

[27]  H. Sauerwein,et al.  Immunoaffinity chromatography and a biotin-streptavidin amplified enzymeimmunoassay for sensitive and specific estimation of estradiol-17 beta. , 1990, Journal of steroid biochemistry.

[28]  E. Froesch,et al.  Insulin-like growth factors I and II in healthy man. Estimations of half-lives and production rates. , 1989, Acta endocrinologica.