Imaging of hemoglobin oxygen saturation variations in single vessels in vivo using photoacoustic microscopy

Photoacoustic microscopy was used to noninvasively image variations in hemoglobin oxygen saturation (SO_2) in the subcutaneous microvasculature of rats in vivo. In phantom tests, the calculated concentration fractions of red ink in double-ink mixtures matched the actual values with a 1% error. In ex vivo studies, the calculated SO_2 in bovine blood agreed with the standard spectrophotometric measurements within a 4% systematic difference. In in vivo studies, arteries and veins were separated based on the measured SO_2 values and variations in SO_2 between different physiological states (hyperoxia, normoxia, and hypoxia) were imaged in single blood vessels.

[1]  Lihong V. Wang,et al.  In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy. , 2006, Optics express.

[2]  Lihong V. Wang,et al.  Imaging acute thermal burns by photoacoustic microscopy. , 2006, Journal of biomedical optics.

[3]  Lihong V. Wang,et al.  Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging , 2006, Nature Biotechnology.

[4]  Geng Ku,et al.  Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography. , 2006, Journal of biomedical optics.

[5]  Lihong V. Wang,et al.  Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo , 2006, SPIE BiOS.

[6]  Lihong V. Wang,et al.  In vivo dark-field reflection-mode photoacoustic microscopy. , 2005, Optics letters.

[7]  Suzanne E. Welcome,et al.  Longitudinal Mapping of Cortical Thickness and Brain Growth in Normal Children , 2022 .

[8]  D. Abbott,et al.  Functional imaging of intratumoral hypoxia. , 2004, Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging.

[9]  M. Udo,et al.  Effect of hypoxia on arterial and venous blood levels of oxygen, carbon dioxide, hydrogen ions and lactate during incremental forearm exercise , 2004, European Journal of Applied Physiology and Occupational Physiology.

[10]  Thomas A. Mustoe, MD, FACS,et al.  Oxygen in Wound Healing—More than a Nutrient , 2004, World Journal of Surgery.

[11]  Lihong V. Wang,et al.  Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain , 2003, Nature Biotechnology.

[12]  Melinda Miller Holt,et al.  Statistics and Data Analysis From Elementary to Intermediate , 2001, Technometrics.

[13]  Seong-Gi Kim,et al.  Effect of hyperoxia, hypercapnia, and hypoxia on cerebral interstitial oxygen tension and cerebral blood flow , 2001, Magnetic resonance in medicine.

[14]  Alexander A. Oraevsky,et al.  Noninvasive detection and staging of oral cancer in vivo with confocal optoacoustic tomography , 2000, BiOS.

[15]  W. Zijlstra,et al.  Visible and Near Infrared Absorption Spectra of Human and Animal Haemoglobin : Determination and Application , 2000 .

[16]  A. Grinvald,et al.  Increased cortical oxidative metabolism due to sensory stimulation: implications for functional brain imaging. , 1999, Science.

[17]  A. Tamhane,et al.  Statistics and Data Analysis: From Elementary to Intermediate , 1999 .

[18]  R. Kruger,et al.  Photoacoustic ultrasound (PAUS)--reconstruction tomography. , 1995, Medical physics.

[19]  M. I. Khan,et al.  Photoacoustic "Signatures" of Particulate Matter: Optical Production of Acoustic Monopole Radiation , 1990, Science.

[20]  B Chance,et al.  Optical and Nuclear Magnetic Resonance Studies of Hypoxia in Human Tissue and Tumors a , 1988, Annals of the New York Academy of Sciences.

[21]  A. Tam.  Applications of photoacoustic sensing techniques , 1986 .

[22]  M Meyer,et al.  Mixing technique for study of oxygen-hemoglobin equilibrium: a critical evaluation. , 1978, Journal of applied physiology: respiratory, environmental and exercise physiology.

[23]  Spectrophotometric determination of the oxygen saturation of whole blood. , 1955, The Journal of biological chemistry.

[24]  S. Kety,et al.  THE EFFECTS OF ALTERED ARTERIAL TENSIONS OF CARBON DIOXIDE AND OXYGEN ON CEREBRAL BLOOD FLOW AND CEREBRAL OXYGEN CONSUMPTION OF NORMAL YOUNG MEN. , 1948, The Journal of clinical investigation.