Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep

According to increase of travel, the cases of imported echinococcosis have been increasing in Korea. The present study was undertaken to develop a serodiagnostic system for echinococcosis in Korea. For diagnosis of echinococcosis, the fluid of Echinococcus granulosus hydatid cysts was collected from naturally infected sheep in Uzbekistan. Also serum samples of infected patients who were surgically confirmed were collected in a hospital in Tashkent, Uzbekistan. According to the absorbance of 59 echinococcosis positive and 39 negative control serum samples, the cut-off value was determined as 0.27. The sensitivity and specificity of ELISA with hydatid fluid antigen were 91.5% and 96%, respectively. The antigen cross-reacted with the serum of some cysticercosis or clonorchiasis patients. However, immunoblot analysis on the cystic fluid recognized antigenic proteins of 7-, 16-, and 24-kDa bands in their dominant protein quantity and strong blotting reactivity. In conclusion, the present ELISA system using hydatid cyst fluid antigen from Uzbekistan sheep is sensitive and specific for diagnosis of echinococcosis cases.

[1]  Sung-Tae Hong,et al.  Infection Status of Hydatid Cysts in Humans and Sheep in Uzbekistan , 2013, The Korean journal of parasitology.

[2]  Sung-Tae Hong,et al.  Infection Status of Hydatid Cysts in Humans and Sheep in Uzbekistan , 2013, The Korean journal of parasitology.

[3]  T. Puthanakit,et al.  Fatal Balamuthia Amebic Encephalitis in a Healthy Child: A Case Report with Review of Survival Cases , 2013, The Korean journal of parasitology.

[4]  J. Chai,et al.  Gnathostoma spinigerum Infection in the Upper Lip of a Korean Woman: An Autochthonous Case in Korea , 2013, The Korean journal of parasitology.

[5]  K. Ahn,et al.  An Imported Case of Cystic Echinococcosis in the Liver , 2012, The Korean journal of parasitology.

[6]  K. Ahn,et al.  An Imported Case of Cystic Echinococcosis in the Liver , 2012, The Korean journal of parasitology.

[7]  D. McManus,et al.  Diagnosis, treatment, and management of echinococcosis , 2012, BMJ : British Medical Journal.

[8]  D. McManus,et al.  Diagnosis, treatment, and management of echinococcosis , 2012, BMJ : British Medical Journal.

[9]  A. Maleki,et al.  Cross-Reaction between the Crude Hydatid Cyst Fluid Antigens of Human and Animals Origin in Response to Human IgG Class and Subclasses , 2012, Journal of parasitology research.

[10]  A. Maleki,et al.  Cross-Reaction between the Crude Hydatid Cyst Fluid Antigens of Human and Animals Origin in Response to Human IgG Class and Subclasses , 2012, Journal of parasitology research.

[11]  Su-jin Kim,et al.  Recurrent Hepatic Alveolar Echinococcosis: Report of The First Case in Korea with Unproven Infection Route , 2011, The Korean journal of parasitology.

[12]  Su-jin Kim,et al.  Recurrent Hepatic Alveolar Echinococcosis: Report of The First Case in Korea with Unproven Infection Route , 2011, The Korean journal of parasitology.

[13]  F. Oreyzi,et al.  Diagnostic value of hydatid cyst antigens using western blotting method , 2010 .

[14]  F. Oreyzi,et al.  Diagnostic value of hydatid cyst antigens using western blotting method , 2010 .

[15]  N. Aydın,et al.  [The clinical, serological and morphological analysis of 91 patients with cystic echinococcosis following surgery]. , 2010, Turkiye parazitolojii dergisi.

[16]  N. Aydın,et al.  [The clinical, serological and morphological analysis of 91 patients with cystic echinococcosis following surgery]. , 2010, Turkiye parazitolojii dergisi.

[17]  I. Ciftci,et al.  [A rare cause of obstructive jaundice: fascioliasis]. , 2010, Turkiye parazitolojii dergisi.

[18]  Pedro Moro,et al.  Echinococcosis: a review. , 2009, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[19]  Pedro Moro,et al.  Echinococcosis: a review. , 2009, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[20]  R. Gilman,et al.  Prevention and control of cystic echinococcosis. , 2007, The Lancet. Infectious diseases.

[21]  R. Gilman,et al.  Prevention and control of cystic echinococcosis. , 2007, The Lancet. Infectious diseases.

[22]  A. Ayadi,et al.  Liver cystic echinococcosis: which cysts are correlated with false negative indirect passive hemagglutination (IHA)? , 2007, La Tunisie medicale.

[23]  A. Ayadi,et al.  Liver cystic echinococcosis: which cysts are correlated with false negative indirect passive hemagglutination (IHA)? , 2007, La Tunisie medicale.

[24]  D. McManus,et al.  Recent advances in the immunology and diagnosis of echinococcosis. , 2006, FEMS immunology and medical microbiology.

[25]  D. McManus,et al.  Recent advances in the immunology and diagnosis of echinococcosis. , 2006, FEMS immunology and medical microbiology.

[26]  P. Torgerson,et al.  Present situation of cystic echinococcosis in Central Asia. , 2006, Parasitology international.

[27]  P. Torgerson,et al.  Present situation of cystic echinococcosis in Central Asia. , 2006, Parasitology international.

[28]  P. Deplazes,et al.  Biological, Epidemiological, and Clinical Aspects of Echinococcosis, a Zoonosis of Increasing Concern , 2004, Clinical Microbiology Reviews.

[29]  P. Deplazes,et al.  Biological, Epidemiological, and Clinical Aspects of Echinococcosis, a Zoonosis of Increasing Concern , 2004, Clinical Microbiology Reviews.

[30]  B. Gottstein,et al.  Echinococcus granulosus antigens: Immunoelectrophoretic and Western blot analysis of hydatid cyst fluids , 2004, Parasitology Research.

[31]  B. Gottstein,et al.  Echinococcus granulosus antigens: Immunoelectrophoretic and Western blot analysis of hydatid cyst fluids , 2004, Parasitology Research.

[32]  Yong-hong Zhang,et al.  [Reexamination of specific antibodies in sera of cystic echinococccosis patients with IgG negative seroresponse]. , 2002, Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases.

[33]  Yong-hong Zhang,et al.  [Reexamination of specific antibodies in sera of cystic echinococccosis patients with IgG negative seroresponse]. , 2002, Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases.

[34]  D. Jenkins.  WHO/OIE manual on Echinococcosis in humans and animals: a public health problem of global concern ☆ , 2001 .

[35]  F. Meslin,et al.  WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. , 2001 .

[36]  F. Meslin,et al.  WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. , 2001 .

[37]  A. Osuna,et al.  Specific recognition of hydatid cyst antigens by serum IgG, IgE, and IgA using western blot. , 1997, Journal of clinical laboratory analysis.

[38]  A. Osuna,et al.  Specific recognition of hydatid cyst antigens by serum IgG, IgE, and IgA using western blot. , 1997, Journal of clinical laboratory analysis.