Identification Of Potential Cytotoxic Inhibitors From Physalis Minima

Abstract A phytochemical investigation of Physalis minima led to the isolation of six withanolides, including withanolide E (1), withaperuvin C (2), 4β-hydroxywithanolide E (3), 28-hydroxywithaperuvin C (4), physaperuvin G (5), and 4-deoxywithaperuvin (6). Their chemical structures were elucidated by 1 D-NMR and 2 D-NMR data, as well as comparison with the data reported in the literature. All isolated compounds were evaluated for their cytotoxic activity against HepG2, SK-LU-1, and MCF7 cancer cell lines. As the obtained results, compounds 1 and 3 displayed the strongest cytotoxicity against HepG2, SK-LU-1, and MCF7 cell lines with IC50 value ranging from 0.051 ± 0.004 to 0.86 ± 0.09 μg/mL. Graphical Abstract

[1]  Yanli Liu,et al.  Anti-inflammatory and cytotoxic withanolides from Physalis minima. , 2018, Phytochemistry.

[2]  P. Kiem,et al.  Phytochemical constituents and cytotoxic activity of Physalis angulata L. growing in Vietnam , 2018, Phytochemistry Letters.

[3]  J. Pezzuto,et al.  Withanolides derived from Physalis peruviana (Poha) with potential anti-inflammatory activity. , 2016, Bioorganic & medicinal chemistry letters.

[4]  L. Kong,et al.  Withaphysalin-type withanolides from Physalis minima , 2016 .

[5]  L. Kong,et al.  Withanolides from Physalis minima and their inhibitory effects on nitric oxide production , 2014, Steroids.

[6]  T. S. Muhammad,et al.  Physalin F from Physalis minima L. triggers apoptosis-based cytotoxic mechanism in T-47D cells through the activation caspase-3- and c-myc-dependent pathways. , 2013, Journal of ethnopharmacology.

[7]  S. Sulaiman,et al.  Growth arrest and induction of apoptotic and non-apoptotic programmed cell death by, Physalis minima L. chloroform extract in human ovarian carcinoma Caov-3 cells. , 2010, Journal of ethnopharmacology.

[8]  Atta-ur-rahman,et al.  New leishmanicidal physalins from Physalis minima , 2007, Natural product research.

[9]  P. Houghton,et al.  Cytotoxicity of plants from Malaysia and Thailand used traditionally to treat cancer. , 2005, Journal of ethnopharmacology.

[10]  N. Abdullaev,et al.  Withasteroids ofPhysalis. V. A study of the1H and13C NMR spectra of the withasteroids visconolide and 28-hydroxywithaperuvin C , 1985, Chemistry of Natural Compounds.

[11]  A. M. Abou-Douh New Withanolides and Other Constituents from the Fruit of Withania somnifera , 2002, Archiv der Pharmazie.

[12]  D. Scudiero,et al.  Application of high-throughput, molecular-targeted screening to anticancer drug discovery. , 2002, Current topics in medicinal chemistry.

[13]  M. Kawai,et al.  Upon ‘physalin L’ isolated from Physalis minima , 1996 .

[14]  G. Sen,et al.  Physalin L, a 13,14-seco-16,24 cyclosteroid from Physalis minima , 1995 .

[15]  S. Yahara,et al.  Three New Withanolides, Physagulins A, B and D from Physalis angulata L. , 1992 .

[16]  D. Scudiero,et al.  Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. , 1991, Journal of the National Cancer Institute.

[17]  N. Ser Flavonoids from Physalis minima , 1988 .

[18]  H. Gottlieb,et al.  Withaminimin, a withanolide from Physalis minima , 1987 .

[19]  Y. Oshima,et al.  Withaphysalin E, a withanolide of Physalis minima var. indica , 1987 .

[20]  Y. Oshima,et al.  Structures of Withaperuvin B and C, Withanolides of Physalis peruviana Roots , 1982 .

[21]  S. Kobayashi,et al.  Isolation of 4 beta-hydroxywithanolide E, a new withanolide from Physalis peruviana L. , 1976, Chemical & pharmaceutical bulletin.