Clinical application of collagen membrane with umbilical cord-derived mesenchymal stem cells to repair nasal septal perforation

Objective. We aimed to investigate the clinical efficacy of collagen membrane with umbilical cord-derived mesenchymal stem cells in the endoscopic repair of nasal septal perforation. Methods. We performed a prospective clinical trial between March 2017 and October 2019. Nasal septal perforations were repaired by the endoscopic sandwich technique with the collagen membrane and umbilical cord-derived mesenchymal stem cells. These patients were followed up postoperatively. Their outcomes were comprehensively evaluated by assessing the healing process of the perforations, the visual analog scale (VAS) for nasal discomfort, and the nasal mucociliary transit time (MTT) for the regenerated nasal mucosa. Results. Our study included a total of eight patients with nasal septal perforation (six males and two females, age 36.6 ± 12.8 years, diameter of perforation 1.0 ± 0.2 cm). Seven patients successfully underwent surgical repair. These patients had significantly improved VAS scores 1 month after the operations (1.1 ± 0.4) compared with the preoperative period (5.9 ± 0.7) (P < 0.05). Although the nasal MTT in the nasal septum and the inferior turbinate surface were within the normal limits before the operation and at 1 month after the operation, the postoperative transit time (11.1 ± 2.0 m) was significantly shorter than the preoperative transit time (12.1 ± 2.4 m) (P < 0.05). There were no recurrences of perforation, scab formations, or epistaxis after the operation. Conclusions. The application of the collagen membrane with umbilical cord-derived mesenchymal stem cells is a simple and feasible endoscopic procedure to repair perforated nasal septa and restore satisfactory functional mucosa.

[1]  Liu Wang,et al.  Allogeneic cell therapy using umbilical cord MSCs on collagen scaffolds for patients with recurrent uterine adhesion: a phase I clinical trial , 2018, Stem Cell Research & Therapy.

[2]  N. Caputo,et al.  Imaging prevalence of nasal septal perforation in an urban population. , 2017, Clinical imaging.

[3]  R. Haye,et al.  Surgical closure of nasal septal perforation. Early and long term observations. , 2011, Rhinology.

[4]  Zhifeng Xiao,et al.  The promotion of neural regeneration in an extreme rat spinal cord injury model using a collagen scaffold containing a collagen binding neuroprotective protein and an EGFR neutralizing antibody. , 2010, Biomaterials.

[5]  N. Nardi,et al.  Biology and applications of Mesenchymal Stem Cells , 2010, Science progress.

[6]  D. Watson,et al.  Surgical management of the septal perforation. , 2009, Otolaryngologic clinics of North America.

[7]  S. Sedrakyan,et al.  Stem Cell and Regenerative Science Applications in the Development of Bioengineering of Renal Tissue , 2008, Pediatric Research.

[8]  T. Ochiya,et al.  A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells , 2008, The FEBS journal.

[9]  E. Gócza,et al.  Mesenchymal Stem Cells Cooperate with Bone Marrow Cells in Therapy of Diabetes , 2008, Stem cells.

[10]  B. Lanier,et al.  Pathophysiology and progression of nasal septal perforation. , 2007, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology.

[11]  D. Mantovani,et al.  Biological performances of collagen-based scaffolds for vascular tissue engineering. , 2005, Biomaterials.

[12]  M. Ahmed,et al.  Microwave irradiated collagen tubes as a better matrix for peripheral nerve regeneration , 2005, Brain Research.

[13]  Steven J. Greco,et al.  Neurons Derived From Human Mesenchymal Stem Cells Show Synaptic Transmission and Can Be Induced to Produce the Neurotransmitter Substance P by Interleukin‐1α , 2005, Stem cells.

[14]  J. Mosca,et al.  T cell responses to allogeneic human mesenchymal stem cells: immunogenicity, tolerance, and suppression. , 2005, Journal of biomedical science.

[15]  R. Kridel.  Considerations in the etiology, treatment, and repair of septal perforations. , 2004, Facial plastic surgery clinics of North America.

[16]  Hwal Suh,et al.  Evaluation of antibiotic-loaded collagen-hyaluronic acid matrix as a skin substitute. , 2004, Biomaterials.

[17]  I. Bruce,et al.  Nasal septum buttons: symptom scores and satisfaction , 2002, The Journal of Laryngology & Otology.

[18]  N. Jones,et al.  The investigation of nasal septal perforations and ulcers , 2001, The Journal of Laryngology & Otology.