Modification and evaluation of direct and high-resolution bone-marrow chromosome preparation techniques.

Chromosome preparations were made from bone-marrow cells from five patients with chronic myelogeneous leukemia and seven healthy donors, using the direct technique and two methotrexate techniques after slight modifications. The parameters used for comparing these techniques were the length of chromosome no. 1 and the area of the mitotic figure. These were determined using a computer-based interactive image analysis system. Significant differences were found in both parameters when comparing the three techniques. In the methotrexate technique, which gave the longest chromosomes and the best chromosome spreads, the incubation period before the methotrexate treatment was extended to overnight and the Colcemid and hypotonic treatments were sligthly shortened. The advantages and disadvantages of short and prolonged culture periods are discussed.

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