In vitro labeling of human umbilical cord mesenchymal stem cells with superparamagnetic iron oxide nanoparticles

Human umbilical cord mesenchymal stem cells (hUC‐MSCs) transplantation has been shown to promote regeneration and neuroprotection in central nervous system (CNS) injuries and neurodegenerative diseases. To develop this approach into a clinical setting it is important to be able to follow the fates of transplanted cells by noninvasive imaging. Neural precursor cells and hematopoietic stem cells can be efficiently labeled by superparamagnetic iron oxide (SPIO) nanoparticle. The purpose of our study was to prospectively evaluate the influence of SPIO on hUC‐MSCs and the feasibility of tracking for hUC‐MSCs by noninvasive imaging. In vitro studies demonstrated that magnetic resonance imaging (MRI) can efficiently detect low numbers of SPIO‐labeled hUC‐MSCs and that the intensity of the signal was proportional to the number of labeled cells. After transplantation into focal areas in adult rat spinal cord transplanted SPIO‐labeled hUC‐MSCs produced a hypointense signal using T2‐weighted MRI in rats that persisted for up to 2 weeks. This study demonstrated the feasibility of noninvasive imaging of transplanted hUC‐MSCs. J. Cell. Biochem. 108: 529–535, 2009. © 2009 Wiley‐Liss, Inc.

[1]  S. Feng,et al.  Human umbilical cord mesenchymal stem cells and the treatment of spinal cord injury , 2009, Chinese medical journal.

[2]  M. Ko,et al.  Transplantation of Human Umbilical Mesenchymal Stem Cells from Wharton's Jelly after Complete Transection of the Rat Spinal Cord , 2008, PloS one.

[3]  A. D. Dei Tos,et al.  Atypical cystic meningioma overexpressing AQP1 in early infancy: case report with literature review , 2008, Acta paediatrica.

[4]  Peter M Jakob,et al.  Iron oxide labelling of human mesenchymal stem cells in collagen hydrogels for articular cartilage repair. , 2008, Biomaterials.

[5]  M. Weiss,et al.  Concise Review: Wharton's Jelly‐Derived Cells Are a Primitive Stromal Cell Population , 2008, Stem cells.

[6]  M. Neri,et al.  Efficient In Vitro Labeling of Human Neural Precursor Cells with Superparamagnetic Iron Oxide Particles: Relevance for In Vivo Cell Tracking , 2008, Stem cells.

[7]  Z. Rumboldt,et al.  Atypical Cystic Meningioma of the Trigeminal Nerve in a Pediatric Patient , 2008, American Journal of Neuroradiology.

[8]  Yu-guang Liu,et al.  Clinical Features and Treatment of Meningiomas in Children: Report of 12 Cases and Literature Review , 2008, Pediatric Neurosurgery.

[9]  Marcello Cadioli,et al.  Magnetic Resonance‐Based Tracking and Quantification of Intravenously Injected Neural Stem Cell Accumulation in the Brains of Mice with Experimental Multiple Sclerosis , 2007, Stem cells.

[10]  Michael S Detamore,et al.  A comparison of human umbilical cord matrix stem cells and temporomandibular joint condylar chondrocytes for tissue engineering temporomandibular joint condylar cartilage. , 2007, Tissue engineering.

[11]  B. Scheithauer,et al.  The 2007 WHO Classification of Tumours of the Central Nervous System , 2007, Acta Neuropathologica.

[12]  C. Werner,et al.  Pathophysiology of traumatic brain injury. , 2007, British journal of anaesthesia.

[13]  R. Silbergleit,et al.  Preconditioning with hyperbaric oxygen attenuates brain edema after experimental intracerebral hemorrhage. , 2007, Neurosurgical focus.

[14]  Gyu Hwan Park,et al.  Role of p38 mapk on the down-regulation of matrix metallo-proteinase-9 expression in rat astrocytes , 2007, Archives of pharmacal research.

[15]  A. Stepulak,et al.  The role of matrix metalloproteinases in infant traumatic brain injury , 2007, Neurobiology of Disease.

[16]  L. Xiong,et al.  Preconditioning with hyperbaric oxygen induces tolerance against oxidative injury via increased expression of heme oxygenase-1 in primary cultured spinal cord neurons. , 2007, Life sciences.

[17]  Bin Zhou,et al.  In vitro and in vivo differentiation of human umbilical cord derived stem cells into endothelial cells , 2007, Journal of cellular biochemistry.

[18]  S. Karahuseyinoglu,et al.  Biology of Stem Cells in Human Umbilical Cord Stroma: In Situ and In Vitro Surveys , 2007, Stem cells.

[19]  J. Bulte,et al.  Serial in vivo MR tracking of magnetically labeled neural spheres transplanted in chronic EAE mice , 2007, Magnetic resonance in medicine.

[20]  Claudia J. Bode,et al.  Potential Treatment of Cerebral Global Ischemia with Oct‐4+ Umbilical Cord Matrix Cells , 2007, Stem cells.

[21]  M. Conconi,et al.  CD105(+) cells from Wharton's jelly show in vitro and in vivo myogenic differentiative potential. , 2006, International journal of molecular medicine.

[22]  H. Kulaçoğlu,et al.  Hyperbaric oxygenation attenuates renal ischemia-reperfusion injury in rats. , 2006, Transplantation proceedings.

[23]  C. Clarke.  Acute mountain sickness: medical problems associated with acute and subacute exposure to hypobaric hypoxia , 2006, Postgraduate Medical Journal.

[24]  J. Greenman,et al.  Hyperbaric oxygen: a new drug in myocardial revascularization and protection? , 2006, Cardiovascular revascularization medicine : including molecular interventions.

[25]  L. Xiong,et al.  Hyperbaric Oxygen Preconditioning Induces Tolerance against Spinal Cord Ischemia by Upregulation of Antioxidant Enzymes in Rabbits , 2006, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[26]  M. Weiss,et al.  Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson's Disease , 2006, Stem cells.

[27]  H. Naganuma,et al.  Atypical meningioma with large cyst. Case report. , 2006, Neurologia medico-chirurgica.

[28]  C. Piantadosi,et al.  A comparison of hyperbaric oxygen versus hypoxic cerebral preconditioning in neonatal rats , 2006, Brain Research.

[29]  N. Fayed,et al.  Evidence of brain damage after high-altitude climbing by means of magnetic resonance imaging. , 2006, The American journal of medicine.

[30]  J. Soustiel,et al.  Hyperbaric oxygen therapy reduces neuroinflammation and expression of matrix metalloproteinase‐9 in the rat model of traumatic brain injury , 2006, Neuropathology and applied neurobiology.

[31]  张庆俊,et al.  Evaluation of neurological function recovery following human umbilical cord mesenchymal stem cells transplantation to injured spinal cord in rats , 2006 .

[32]  M. Ko,et al.  Conversion of Human Umbilical Cord Mesenchymal Stem Cells in Wharton's Jelly to Dopaminergic Neurons In Vitro: Potential Therapeutic Application for Parkinsonism , 2006, Stem cells.

[33]  O. Tasaki,et al.  Changes in cerebral blood flow from the acute to the chronic phase of severe head injury. , 2005, Journal of neurotrauma.

[34]  Q. Xie,et al.  Human umbilical cord Wharton's Jelly-derived mesenchymal stem cells differentiation into nerve-like cells. , 2005, Chinese medical journal.

[35]  P. McCollum,et al.  Pretreatment with hyperbaric oxygen and its effect on neuropsychometric dysfunction and systemic inflammatory response after cardiopulmonary bypass: a prospective randomized double-blind trial. , 2005, The Journal of thoracic and cardiovascular surgery.

[36]  O. Alonso,et al.  Influence of Therapeutic Hypothermia on Matrix Metalloproteinase Activity after Traumatic Brain Injury in Rats , 2005, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[37]  Shi-Yau Yu,et al.  Preconditioned hyperbaric oxygenation protects the liver against ischemia-reperfusion injury in rats. , 2005, The Journal of surgical research.

[38]  M. Bock,et al.  Physical and Biological Characterization of Superparamagnetic Iron Oxide- and Ultrasmall Superparamagnetic Iron Oxide-Labeled Cells: A Comparison , 2005, Investigative radiology.

[39]  Shin Jung,et al.  Clinical and histopathological analysis of cystic meningiomas , 2005, Journal of Clinical Neuroscience.

[40]  Dolores Baksh,et al.  Human Umbilical Cord Perivascular (HUCPV) Cells: A Source of Mesenchymal Progenitors , 2005, Stem cells.

[41]  Chun-Chieh Huang,et al.  Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord , 2004, Stem cells.

[42]  Andras Lakatos,et al.  Superparamagnetic Iron Oxide-Labeled Schwann Cells and Olfactory Ensheathing Cells Can Be Traced In Vivo by Magnetic Resonance Imaging and Retain Functional Properties after Transplantation into the CNS , 2004, The Journal of Neuroscience.

[43]  Ole Isacson,et al.  Intrastriatal Transforming Growth Factor α Delivery to a Model of Parkinson's Disease Induces Proliferation and Migration of Endogenous Adult Neural Progenitor Cells without Differentiation into Dopaminergic Neurons , 2004, The Journal of Neuroscience.

[44]  F. Gage,et al.  Robust in vivo gene transfer into adult mammalian neural stem cells by lentiviral vectors. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[45]  M. Min,et al.  Transformation of Human Umbilical Mesenchymal Cells into Neurons in vitro , 2004, Journal of Biomedical Science.

[46]  Trevor Douglas,et al.  In vivo magnetic resonance tracking of olfactory ensheathing glia grafted into the rat spinal cord , 2004, Experimental Neurology.

[47]  Scott A Gallagher,et al.  High-altitude illness. , 2004, Emergency medicine clinics of North America.

[48]  P. Arlotta,et al.  Constitutive and Induced Neurogenesis in the Adult Mammalian Brain: Manipulation of Endogenous Precursors toward CNS Repair , 2004, Developmental Neuroscience.

[49]  Z. Zuo,et al.  Morphine Preconditions Purkinje Cells against Cell Death under In Vitro Simulated Ischemia–Reperfusion Conditions , 2004, Anesthesiology.

[50]  P. Lai,et al.  Magnetic resonance imaging and diffusion-weighted images of cystic meningioma: correlating with histopathology. , 2004, Clinical imaging.

[51]  R. Silbergleit,et al.  Neuroprotective effects of hyperbaric oxygen treatment in experimental focal cerebral ischemia are associated with reduced brain leukocyte myeloperoxidase activity , 2003, Brain Research.

[52]  M. Weiss,et al.  Matrix Cells from Wharton's Jelly Form Neurons and Glia , 2003, Stem cells.

[53]  Y. Romanov,et al.  Searching for Alternative Sources of Postnatal Human Mesenchymal Stem Cells: Candidate MSC‐Like Cells from Umbilical Cord , 2003, Stem cells.

[54]  Silvia Fischer,et al.  Hypoxia-induced vascular endothelial growth factor expression causes vascular leakage in the brain. , 2002, Brain : a journal of neurology.

[55]  L. Xiong,et al.  Preconditioning with Hyperbaric Oxygen and Hyperoxia Induces Tolerance against Spinal Cord Ischemia in Rabbits , 2002, Anesthesiology.

[56]  H. Nawashiro,et al.  Preferential Conditions for and Possible Mechanisms of Induction of Ischemic Tolerance by Repeated Hyperbaric Oxygenation in Gerbil Hippocampus , 2001, Neurosurgery.

[57]  L. Xiong,et al.  Hyperbaric oxygen preconditioning induces neuroprotection against ischemia in transient not permanent middle cerebral artery occlusion rat model. , 2000, Chinese medical journal.

[58]  M. Moskowitz,et al.  Hyperbaric oxygenation induced tolerance against focal cerebral ischemia in mice is strain dependent , 2000, Brain Research.

[59]  M. Krug,et al.  The importance of free hydroxyl radicals to hypoxia preconditioning , 2000, Brain Research.

[60]  C. Gladson,et al.  Increased injury following intermittent fetal hypoxia-reoxygenation is associated with increased free radical production in fetal rabbit brain. , 1999, Journal of neuropathology and experimental neurology.

[61]  D. Sessler,et al.  Postoperative pain and subcutaneous oxygen tension , 1999, The Lancet.

[62]  C. Robertson,et al.  Regional changes in cerebral extracellular glucose and lactate concentrations following severe cortical impact injury and secondary ischemia in rats. , 1999, Journal of neurotrauma.

[63]  R. Keep,et al.  Erythrocytes and delayed brain edema formation following intracerebral hemorrhage in rats. , 1998, Journal of neurosurgery.

[64]  I. Nagata,et al.  Infarct tolerance against temporary focal ischemia following spreading depression in rat brain , 1998, Brain Research.

[65]  D. M. Feeney,et al.  d-Amphetamine attenuates decreased cerebral glucose utilization after unilateral sensorimotor cortex contusion in rats , 1997, Brain Research.

[66]  K. Mori,et al.  Intraventricular, cystic, atypical meningioma. , 1997, Neurologia medico-chirurgica.

[67]  H. Nawashiro,et al.  TNF-α Pretreatment Induces Protective Effects against Focal Cerebral Ischemia in Mice , 1997 .

[68]  E R Laws,et al.  Factors associated with survival in patients with meningioma. , 1997, Journal of neurosurgery.

[69]  H. Nawashiro,et al.  Repeated hyperbaric oxygen induces ischemic tolerance in gerbil hippocampus , 1996, Brain Research.

[70]  M. Apuzzo,et al.  Magnetic resonance imaging of cystic meningiomas and its surgical implications. , 1995, Neurosurgery.

[71]  O. De Jesus,et al.  Cystic meningiomas: a review. , 1995, Neurosurgery.

[72]  A. Mahmood,et al.  Atypical and malignant meningiomas: a clinicopathological review. , 1993 .

[73]  C. Zee,et al.  Magnetic Resonance Imaging and Pathological Correlates of Meningiomas , 1992 .

[74]  A. Ruelle,et al.  "True" cystic meningioma. , 1985, Journal of neurology, neurosurgery, and psychiatry.

[75]  R. Chan,et al.  Intracranial meningiomas in childhood. , 1984, Surgical neurology.

[76]  S. Batnitzky,et al.  Cystic lesions associated with intracranial meningiomas. , 1979, Neurosurgery.

[77]  T. Zemunik,et al.  Effect of hyperbaric oxygenation on the regeneration of the liver after partial hepatectomy in rats. , 2004, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[78]  J. Weber,et al.  Intraoperative management of cystic meningiomas , 2002, Neurosurgical Review.

[79]  T. Seto,et al.  Ginkgo biloba for the prevention of severe acute mountain sickness (AMS) starting one day before rapid ascent. , 2002, High altitude medicine & biology.

[80]  M A Edgar,et al.  Appearance of meningiomas on diffusion-weighted images: correlating diffusion constants with histopathologic findings. , 2001, AJNR. American journal of neuroradiology.

[81]  P. Weinstein,et al.  Reversible middle cerebral artery occlusion without craniectomy in rats. , 1989, Stroke.