Real time analysis of competitive binding using grating coupler immunosensors for pesticide detection

Abstract Real time analysis of binding events is one step forward from immunoassays to immunosensors. Using grating couplers as transducers, the approach is adapted to hapten detection by a competitive assay format. A special advantage of real time analysis is the information obtained from the kinetic behaviour of the immunosystem. In this study we show that the interpretation of the dynamic system response compared to a quasi-stationary one shifts the detection limit to lower hapten concentrations, and shortens the assay time. In the case of s-triazines, it was found that the detection limit decreases by 4 times, while incubation time is shortened tenfold to 3 min.

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