This paper describes an innovative biotechnology-utilizing, microbiologically-induced mineral precipitation for concrete remediation. Calcite precipitation induced by Bacillus pasteurii was studied in 2 types of portland cement mortar (PCM) specimens: 1 prepared from mixing with micro-organisms, and the other with simulated cracks filled with microbial mixtures. Study results show that there was a significant increase in compressive strength of the PCM cubes containing lower concentrations of live cels. Compressive strengths of the cubes containing live or dead cell mass, however, decreased as cell concentrations and curing time increased, suggesting the interference of mortar integrity by biomass. Cracks filled with bacteria and sand demonstrated a significant increase in compressive strength and stiffness values when compared with those without cells.